Autonomous oscillation/separation of cell density artificially induced by optical interlink feedback as designed interaction between two isolated microalgae chips.

Abstract:

:We demonstrate a designed interaction between two isolated cell populations of Euglena gracilis and Chlamydomonas reinhardtii, separately confined in two 25-square micro-aquariums of lab-on-chip size. The interaction was realized by interlinking two identical optical feedback systems, which measured the cell distribution. To analyze the cell populations, we measured the cell distribution in the 25 squares and irradiated the cells with a blue light pattern as an external stimulus. The cell distribution dataset was exchanged between the two systems. Governed by a designed interaction algorithm, the feedback systems produced a dynamic blue light illumination pattern that evoked the photophobic responses of both species. We also induced autonomous cell density oscillation and cell distribution separation and clustering, and analyzed how the types and diversities of the photophobic responses affected the oscillation period and separation and clustering. We conclude that artificial interlink feedback is a promising method for investigating diverse cell-cell interactions in ecological communities, and for developing soft-computing applications with living cells.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Ozasa K,Won J,Song S,Maeda M

doi

10.1038/srep24602

subject

Has Abstract

pub_date

2016-04-21 00:00:00

pages

24602

issn

2045-2322

pii

srep24602

journal_volume

6

pub_type

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