Abstract:
:In this article the notion of metabolic turnover is revisited in the light of recent results of out-of-equilibrium thermodynamics. By means of Monte Carlo methods we perform an exact sampling of the enzymatic fluxes in a genome scale metabolic network of E. coli in stationary growth conditions from which we infer the metabolites turnover times. However the latter are inferred from net fluxes, and we argue that this approximation is not valid for enzymes working nearby thermodynamic equilibrium. We recalculate turnover times from total fluxes by performing an energy balance analysis of the network and recurring to the fluctuation theorem. We find in many cases values one of order of magnitude lower, implying a faster picture of intermediate metabolism.
journal_name
Phys Bioljournal_title
Physical biologyauthors
De Martino Ddoi
10.1088/1478-3975/13/1/016003subject
Has Abstractpub_date
2016-01-29 00:00:00pages
016003issue
1eissn
1478-3967issn
1478-3975journal_volume
13pub_type
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