Abstract:
STUDY DESIGN:A study examining the clinical protocol of scoliosis wound irrigation, demonstrating povidone-iodine's (PVI) effect on human osteoblast cells. Primary and immortal cell line osteoblasts were treated with 0.35% PVI for 3 minutes, and analyzed for proliferation rate, oxidative capacity, and mineralization. OBJECTIVE:To model spinal wound irrigation with dilute PVI in vitro, in order to investigate the effect of PVI on osteoblast proliferation, metabolism, and bone mineralization. SUMMARY OF BACKGROUND DATA:Previously PVI irrigation has been proposed as a safe and effective practice to avoid bacterial growth after spinal surgery. However, recent evidence in multiple cell types suggests that PVI has a deleterious effect on cellular viability and cellular function. METHODS:Primary and immortal human osteoblast cells were exposed to either phosphate buffered saline control or with 0.35% PVI for 3 minutes. Cellular proliferation was measured over the duration of 7 days by MTS assay. Oxygen consumption rate, extracellular acidification rate, and proton production rate were analyzed using a Seahorse XF24 Bioanalyzer. Protein expression of the electron transport chain subunits CII-SDHB, CIII-UQRCR2, and CV-ATP5A was measured via Western blotting. Mineralized bone nodules were stained with alizarin red. RESULTS:Expressed as a percentage of normal osteoblast proliferation, osteoblasts exposed to 0.35% PVI exhibited a significant 24% decrease in proliferation after 24 hours. This was a sustained response, resulting in a 72% decline in cellular proliferation at 1 week. There was a significant reduction in oxygen consumption rate, extracellular acidification rate, and proton production rate (P < 0.05), in osteoblasts that had been exposed to 0.35% PVI for 3 minutes, coupled with a marked reduction in the protein expression of CII-SDHB. Osteoblasts exposed to 0.35% PVI exhibited reduced bone nodule mineralization compared to control phosphate buffered saline exposed osteoblasts (P < 0.01). CONCLUSION:PVI has a rapid and detrimental effect on human osteoblast cellular proliferation, metabolic function, and bone nodule mineralization. LEVEL OF EVIDENCE:NA.
journal_name
Spine (Phila Pa 1976)journal_title
Spineauthors
Newton Ede MP,Philp AM,Philp A,Richardson SM,Mohammad S,Jones SWdoi
10.1097/BRS.0000000000001332subject
Has Abstractpub_date
2016-05-01 00:00:00pages
729-34issue
9eissn
0362-2436issn
1528-1159journal_volume
41pub_type
杂志文章相关文献
SPINE文献大全abstract::Thirty-three patients with unstable burst fractures of the lower thoracic and lumbar spine were treated with a reduction-fixation system. The new system is used both as a reduction and a fixation device for disorders of the lower thoracic and lumbar spine. In treatment of spinal fractures, it provides symmetric lordot...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-199208000-00004
更新日期:1992-08-01 00:00:00
abstract:STUDY DESIGN:To analyze Boston brace biomechanics, pressure measurements and finite element simulations were done on 12 adolescent idiopathic scoliosis patients. OBJECTIVES:The aim was to analyze the Boston brace effectiveness using a finite element model and experimental measurements. SUMMARY OF BACKGROUND DATA:Ther...
journal_title:Spine
pub_type: 临床试验,杂志文章
doi:10.1097/01.BRS.0000083165.93936.6D
更新日期:2003-08-01 00:00:00
abstract:STUDY DESIGN:Prospective multicenter observation. OBJECTIVE:To determine the validity of 3 commercially available at recording thoracic-lumbar-sacral orthosis (TLSO) wearing time of children with spinal cord injury (SCI) and to assess each monitor's function during daily activities. SUMMARY OF BACKGROUND DATA:A major...
journal_title:Spine
pub_type: 杂志文章,多中心研究
doi:10.1097/BRS.0b013e318178864e
更新日期:2008-06-15 00:00:00
abstract:STUDY DESIGN:This is a technical note and review of the literature. OBJECTIVE:We propose to describe a revised surgical technique of C1 lateral mass screw insertion with protection of C1-C2 venous sinus surrounding the C2 nerve root. SUMMARY OF BACKGROUND DATA:During C1 lateral mass screw insertion and in posterior C...
journal_title:Spine
pub_type: 杂志文章,评审
doi:10.1097/BRS.0b013e3181e215ff
更新日期:2010-10-01 00:00:00
abstract::During the summers of 1987 and 1988, 14 cervical spine injuries sustained during bodysurfing accidents at the beaches around Ocean City, Maryland presented to our Neurotrauma service. All the patients were men, predominantly in their middle years (mean age = 40), and otherwise healthy. Likely mechanisms of injury sugg...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-199203000-00002
更新日期:1992-03-01 00:00:00
abstract:STUDY DESIGN:A case report. OBJECTIVE:The aim of this study was to describe a technique of temporary distal fixation to the ilium to salvage an acute L4 fracture following a T3-L4 kyphoscoliosis correction. SUMMARY OF BACKGROUND DATA:Pedicle fracture is a possible complication in pedicle screw fixation for scoliosis,...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0000000000001786
更新日期:2017-03-01 00:00:00
abstract:STUDY DESIGN:Retrospective review. OBJECTIVE:To identify the incidence and characteristics of rib anomalies in patients with congenital scoliosis (CS) in a Chinese population. SUMMARY OF BACKGROUND DATA:Rib deformities and intraspinal anomalies often coexist in individuals with CS. Rib deformities may assist in diagn...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0000000000000008
更新日期:2013-12-15 00:00:00
abstract:STUDY DESIGN:Prospective, single-cohort study. OBJECTIVE:To evaluate thoracic and thoracolumbar scoliosis using the Kaneda anterior spine dual-rod system (KASS). SUMMARY OF BACKGROUND DATA:In selected cases, anterior correction of scoliosis has several advantages over the traditional posterior instrumentation. Other ...
journal_title:Spine
pub_type: 临床试验,杂志文章
doi:10.1097/01.brs.0000170291.77450.8b
更新日期:2005-07-15 00:00:00
abstract::Automated percutaneous discectomy is a new, safe procedure for treating herniated lumbar discs still contained by the annulus or posterior longitudinal ligament. In 1985, one of the authors reported a percutaneous nucleus aspiration technique using a 2-mm aspiration probe. This small probe produced minimal tissue dama...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-199103000-00018
更新日期:1991-03-01 00:00:00
abstract::The purpose of this study was to describe trunk strength and lumbar paraspinal muscle activity across five angles of flexion during isometric exercise and rest in chronic low-back pain patients and control subjects. High muscle tension as measured by surface integrated electromyography is predicted by a muscle spasm m...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-199302000-00012
更新日期:1993-02-01 00:00:00
abstract:STUDY DESIGN:Narrative review of the literature and commentary. OBJECTIVE:To discuss from an epidemiological and sociological perspective whether the early clinical management of whiplash-associated disorders can lead to iatrogenic disability. SUMMARY OF BACKGROUND DATA:There is a lack of evidence supporting the effe...
journal_title:Spine
pub_type: 杂志文章,评审
doi:10.1097/BRS.0b013e3182388d32
更新日期:2011-12-01 00:00:00
abstract:STUDY DESIGN:Structural investigation of mechanically induced herniations in ovine lumbar motion segments. OBJECTIVE:This new study addresses the question of whether there are regions other than the posterior and posterolateral aspects that are implicated in the initiation of disc disruption and herniation. SUMMARY O...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0000000000002181
更新日期:2017-11-01 00:00:00
abstract:STUDY DESIGN:Isolated tubercular involvement of craniovertebral junction in a human immunodeficiency virus-positive patient causing paraplegia and sudden death with radiologic features is presented. OBJECTIVES:Isolated involvement of craniovertebral junction by tuberculosis causing quadriparesis is a rare entity. The ...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/01.BRS.0000083257.63544.A9
更新日期:2003-08-01 00:00:00
abstract:STUDY DESIGN:Case report. OBJECTIVE:The authors present a case of atypical Burkitt's lymphoma with multiple epidural involvements. SUMMARY OF BACKGROUND DATA:Spinal cord compression in children is an emergency that requires urgent attention to minimize neurologic dysfunction. Although it is not life-threatening in mo...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/01.BRS.0000105540.76773.9C
更新日期:2004-01-01 00:00:00
abstract:STUDY DESIGN:Retrospective review of a prospectively collected, multicentered database from the Scoliosis Research Society. OBJECTIVES:To evaluate incidences of complications in a series of spinal fusions for Scheuermann kyphosis (SK) and to assess whether the incidence of complications is associated with patient age ...
journal_title:Spine
pub_type: 杂志文章,多中心研究
doi:10.1097/BRS.0b013e3181c47f0f
更新日期:2010-01-01 00:00:00
abstract:STUDY DESIGN:This case study is designed to report the previously unreported specific complication of acute cauda equina compression after decompression surgery for spinal stenosis. OBJECTIVES:To inform others of the possibility of such a complication; to suggest a possible mechanism for this situation, namely hourgla...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-199511001-00017
更新日期:1995-11-15 00:00:00
abstract:STUDY DESIGN:Retrospective cohort study. OBJECTIVE:This study aimed to identify the rates and causes of unplanned hospital readmission at 30 days and 1 year after surgical treatment of primary and metastatic spinal tumors. SUMMARY OF BACKGROUND DATA:Primary spine tumors and non-spine tumors metastatic to the spine ca...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0000000000000517
更新日期:2014-10-01 00:00:00
abstract:STUDY DESIGN:A retrospective study by an independent observer of a consecutive series of 67 cases of adolescent idiopathic scoliosis presenting with a King II curve pattern. OBJECTIVES:To demonstrate the validity of a selective thoracic fusion as a treatment of King II curves with special attention to immediate postop...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-199810010-00005
更新日期:1998-10-01 00:00:00
abstract:STUDY DESIGN:Prospective inception cohort study. OBJECTIVE:To investigate prospectively whether pain-related fear predicts future perceived disability and participation in patients with acute low back pain (LBP). SUMMARY OF BACKGROUND DATA:There are indications that fear of movement/(re)injury, as measured by the Tam...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/01.brs.0000203709.65384.9d
更新日期:2006-03-15 00:00:00
abstract::In 30 patients undergoing Cotrel-Dubousset instrumentation, computerized tomography was performed before and after surgery. The RAsag-angle was measured for each vertebral level. Coronal and sagittal translation were evaluated in the same computerized tomographic scans by means of a new method. Based on the hooks used...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-199208001-00009
更新日期:1992-08-01 00:00:00
abstract:STUDY DESIGN:An in vivo study to measure rat spinal cord blood flow in real-time at the site of compression using a newly developed device. OBJECTIVES:To evaluate the change in thoracic spinal cord blood flow by compression force and to clarify the association between blood flow recovery and motor deficiency after a s...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0b013e3181316310
更新日期:2007-08-15 00:00:00
abstract:STUDY DESIGN:A 12-year prospective study of pre-existing and de novo degenerative lumbar scoliosis (DLS) among community-based female volunteers. OBJECTIVE:To investigate serial entire spine radiographs of healthy female volunteers and to clarify radiographical characteristics and predictors of pre-existing and de nov...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0b013e3182575eaa
更新日期:2012-09-15 00:00:00
abstract:STUDY DESIGN:T1rho relaxation was quantified and correlated with intervertebral disc degeneration and proteoglycan content in cadaveric human lumbar spine tissue. OBJECTIVE:To show the use of T1rho-weighted magnetic resonance imaging (MRI) for the assessment of degeneration and proteoglycan content in the human interv...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/01.brs.0000217708.54880.51
更新日期:2006-05-15 00:00:00
abstract::Cyclic axial compression-tension tests and cyclic torsional tests were performed on ten fresh human L4-5 functional spinal units to investigate the structural effects of the posterior elements on the mechanical properties of L4-5 functional spinal units. The stiffness of the functional spinal unit increased with the i...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-199211000-00014
更新日期:1992-11-01 00:00:00
abstract:STUDY DESIGN:An established rabbit posterolateral lumbar fusion model was used to evaluate the ability of osteogenic protein-1 to overcome the inhibitory effect of nicotine. OBJECTIVE:To determine whether osteogenic protein-1 should be considered as a bone graft alternative for the patient who smokes. SUMMARY OF BACK...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-200108010-00004
更新日期:2001-08-01 00:00:00
abstract:STUDY DESIGN:Systematic literature review. OBJECTIVE:To categorize published evidence systematically for lumbar fusion for chronic low back pain (LBP) in order to provide an updated and comprehensive analysis of the clinical outcomes. SUMMARY OF BACKGROUND DATA:Despite a large number of publications of outcomes of sp...
journal_title:Spine
pub_type: 杂志文章,评审
doi:10.1097/BRS.0b013e3182877f11
更新日期:2013-04-01 00:00:00
abstract:STUDY DESIGN:In this study, normal intervertebral disc (IVD) tissues and degenerative human IVD tissues were compared for presence of fibronectin (FN) mRNA splice variants and for FN fragments (FN-f). OBJECTIVE:To further understand FN RNA splice forms and protein fragments in disc degeneration. SUMMARY OF BACKGROUND...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0b013e3181c6ef1a
更新日期:2010-08-01 00:00:00
abstract:STUDY DESIGN:Retrospective radiographical review. OBJECTIVE:To evaluate the outcome of selective thoracic fusion (STF) by using the Guan-Din method for the treatment of major thoracic compensatory lumbar (MTCL) curves. SUMMARY OF BACKGROUND DATA:Performing STF for MTCL curves is to minimize the loss of lumbar motion ...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0000000000000111
更新日期:2014-02-15 00:00:00
abstract:STUDY DESIGN:A retrospective study of surgical outcome of 33 patients with irreducible atlantoaxial dislocation (IAAD). OBJECTIVE:To evaluate the safety efficacy of one stage anterior release and posterior fixation and fusion to reduce and stabilize IAAD. SUMMARY OF BACKGROUND DATA:The traditional treatment of sympto...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/01.brs.0000217686.80327.e4
更新日期:2006-05-15 00:00:00
abstract:STUDY DESIGN:A randomized controlled trial. OBJECTIVE:To investigate the effect of muscle relaxant for muscle blood flow at the trunk muscle in patients with chronic low back pain (LBP). SUMMARY OF BACKGROUND DATA:Paraspinal muscle function is widely believed to play a role and considered to be of etiologic significa...
journal_title:Spine
pub_type: 杂志文章,随机对照试验
doi:10.1097/BRS.0b013e318166e051
更新日期:2008-03-15 00:00:00