IGF-1 Regulates the Extracellular Level of Active MMP-2 and Promotes Müller Glial Cell Motility.

abstract：PURPOSE:The increase in intraocular pressure in primary open-angle glaucoma (POAG) may involve transforming growth factor (TGF)-beta2 signaling, as TGF-beta2 is found in higher amounts than normal in the aqueous humor of patients with POAG. In vitro, TGF-beta2 causes an accumulation of extracellular matrix (ECM) in the...

journal_title：Investigative ophthalmology & visual science

authors： Fuchshofer R,Yu AH,Welge-Lüssen U,Tamm ER

更新日期：2007-02-01 00:00:00

abstract：PURPOSE:To demonstrate the presence of corneal stromal precursors that express neural markers in vitro. METHODS:To isolate sphere-forming cells, human corneal stromal cells were subjected to a reaggregation-free neurosphere assay in medium containing methylcellulose gel matrix. To promote differentiation, the isolated...

journal_title：Investigative ophthalmology & visual science

authors： Uchida S,Yokoo S,Yanagi Y,Usui T,Yokota C,Mimura T,Araie M,Yamagami S,Amano S

更新日期：2005-05-01 00:00:00

abstract：PURPOSE:A subset of patients with anterior uveitis express the marker, perinuclear anti-neutrophil cytoplasmic antibody (pANCA). In this study, recombinantly isolated pANCA monoclonal antibodies were used to search for ocular cells expressing the pANCA antigen. METHODS:Paraffin sections of human ocular tissues obtaine...

journal_title：Investigative ophthalmology & visual science

authors： Gordon LK,Eggena M,Targan SR,Braun J

更新日期：1999-05-01 00:00:00

abstract：PURPOSE:Cornea confocal microscopy is emerging as a clinical tool to evaluate the development and progression of diabetic neuropathy. The purpose of these studies was to characterize the early changes in corneal sensitivity and innervation in a rat model of type 1 diabetes in relation to standard peripheral neuropathy ...

journal_title：Investigative ophthalmology & visual science

authors： Davidson EP,Coppey LJ,Yorek MA

更新日期：2012-12-07 00:00:00

abstract：PURPOSE:To design a glucocorticoid-inducible virus vector overexpressing recombinant matrix metalloproteinase 1 (MMP1) and counteract extracellular matrix deposition in the trabecular meshwork only when steroid is present. METHODS:Endogenous MMP1 expression was measured in primary human trabecular meshwork cells (HTM)...

journal_title：Investigative ophthalmology & visual science

authors： Spiga MG,Borrás T

更新日期：2010-06-01 00:00:00

abstract：PURPOSE:The purpose of this study was to determine whether a better IOP reduction can be observed in conscious, normotensive monkeys treated with ONO-9054, a novel dual EP3 and FP receptor agonist, compared with prostaglandin F2α analogs. METHODS:The binding affinities and agonistic activities of ONO-AG-367, a carboxy...

journal_title：Investigative ophthalmology & visual science

authors： Yamane S,Karakawa T,Nakayama S,Nagai K,Moriyuki K,Neki S,Suto F,Kambe T,Hirota Y,Kawabata K

更新日期：2015-04-01 00:00:00

abstract：PURPOSE:To determine some of the mechanisms by which substance P (SP) induces contraction in the isolated rat iris. METHODS:Rings of rat iris were mounted in a 5-ml organ chamber containing Krebs solution at 37 degrees C under basal tension of 75 mg, and isometric tension was recorded. RESULTS:Substance P produced gr...

journal_title：Investigative ophthalmology & visual science

authors： Grumann-Júnior A,Dias MA,Alves RV,Boteon JE,Calixto JB

更新日期：2000-06-01 00:00:00

abstract：PURPOSE:The purpose of this study was to investigate the effect of bovine lactoferrin (BLF) on human corneal epithelial wound healing using an in vitro alkali-induced wound model and to understand its role in promoting wound healing. METHODS:Confluent human corneal limbal epithelial (HCLE) cells wounded using 0.5 micr...

journal_title：Investigative ophthalmology & visual science

authors： Pattamatta U,Willcox M,Stapleton F,Cole N,Garrett Q

更新日期：2009-04-01 00:00:00

abstract：Purpose:Tumor necrosis factor (TNF)-α is upregulated in eyes following corneal alkali injury and contributes to corneal and also retinal damage. Prompt TNF-α inhibition by systemic infliximab ameliorates retinal damage and improves corneal wound healing. However, systemic administration of TNF-α inhibitors carries risk...

journal_title：Investigative ophthalmology & visual science

authors： Zhou C,Robert MC,Kapoulea V,Lei F,Stagner AM,Jakobiec FA,Dohlman CH,Paschalis EI

更新日期：2017-01-01 00:00:00

abstract：PURPOSE:To determine and compare gene expression patterns in the whole retina and retinal ganglion cell layer (RGCL) in a rodent glaucoma model. METHODS:IOP was unilaterally elevated in Brown Norway rats (N = 26) by injection of hypertonic saline and monitored for 5 weeks. A cDNA microarray was used on whole retinas f...

journal_title：Investigative ophthalmology & visual science

authors： Guo Y,Cepurna WO,Dyck JA,Doser TA,Johnson EC,Morrison JC

更新日期：2010-06-01 00:00:00

abstract：PURPOSE:To estimate the heritability of the iridotrabecular angle width measured by anterior segment optical coherence tomography (ASOCT) in a classic twin study. METHODS:Twins aged 8 to 16 years were identified from the Guangzhou Twin Registry. ASOCT was used to obtain one horizontal scan, and the images were analyze...

journal_title：Investigative ophthalmology & visual science

authors： He M,Ge J,Wang D,Zhang J,Hewitt AW,Hur YM,Mackey DA,Foster PJ

更新日期：2008-04-01 00:00:00

abstract：:After viewing red vertical stripes and green horizontal stripes, the eye subsequently views white vertical stripes as greenish and white horizontal stripes as pinkish. It has been theorized that this phenomenon, known as the McCollough effect, is related to long-tern adaptation of cells tuned for both color and orient...

journal_title：Investigative ophthalmology & visual science

authors： Maguire WM,Meyer GE,Baizer JS

更新日期：1980-03-01 00:00:00

abstract：PURPOSE:The efficacy of the peptide Ac-EEED on reducing cell adhesion and proliferation in vitro and choroidal neovascularization (CNV) in vivo was examined. METHODS:The peptide chimera containing the Ac-EEED sequence was chemically linked to the N terminus of the XMTM delivery peptide from the E(rns) viral surface pr...

journal_title：Investigative ophthalmology & visual science

authors： Caballero S,Yang R,Grant MB,Chaqour B

更新日期：2011-04-16 00:00:00

abstract：PURPOSE:We investigated age-related change in peripapillary retinal nerve fiber layer thicknesses (RNFLT) measured with spectral domain optical coherence tomography (SD-OCT) in healthy individuals. METHODS:In this cross-sectional study, peripapillary RNFL measurement was performed using a Cirrus SD-OCT device. Linear ...

journal_title：Investigative ophthalmology & visual science

authors： Celebi AR,Mirza GE

更新日期：2013-12-11 00:00:00

abstract：PURPOSE:Given reports of corneal edema after endothelial exposure to platelet activating factor (PAF), the authors have investigated whether PAF can affect the function of corneal endothelium in vitro. METHODS:The endothelial side of deepithelialized rabbit corneas was perfused with BSS+ and test agents: PAF, its inac...

journal_title：Investigative ophthalmology & visual science

authors： Zhu Z,Kuang K,Kang F,Li J,Fischbarg J

更新日期：1996-08-01 00:00:00

abstract：PURPOSE:Experimental autoimmune uveoretinitis (EAU) in mice is an important model for elucidating basic mechanisms in autoimmune eye disease. The need for pertussis toxin (PTX) as an additional adjuvant to elicit EAU has limited the usefulness of this model in some types of studies by introducing a pleiotropic factor w...

journal_title：Investigative ophthalmology & visual science

authors： Silver PB,Chan CC,Wiggert B,Caspi RR

更新日期：1999-11-01 00:00:00

abstract：:The mouse retina has been used extensively over the past decades to study both physiologic and pathologic angiogenesis. Over time, various mouse retina models have evolved into well-characterized and robust tools for in vivo angiogenesis research. This article is a review of the angiogenic development of the mouse ret...

journal_title：Investigative ophthalmology & visual science

authors： Stahl A,Connor KM,Sapieha P,Chen J,Dennison RJ,Krah NM,Seaward MR,Willett KL,Aderman CM,Guerin KI,Hua J,Löfqvist C,Hellström A,Smith LE

更新日期：2010-06-01 00:00:00

abstract：Purpose:In many individuals with pattern strabismus, the vertical misalignment varies with horizontal eye position. It has been proposed that these cross-axis effects result from abnormal cross-talk between brainstem structures that would normally encode horizontal and vertical eye position and velocity. The nucleus pr...

journal_title：Investigative ophthalmology & visual science

authors： Pallus A,Walton MMG

更新日期：2020-05-11 00:00:00

abstract：PURPOSE:To determine whether transcorneal electrical stimulation (TES) has neuroprotective effects on the photoreceptors, and whether it slows the rate of decrease of the electroretinogram (ERG) in rhodopsin P347L transgenic (Tg) rabbits. METHODS:Six-week-old Tg rabbits received TES through a contact lens electrode on...

journal_title：Investigative ophthalmology & visual science

authors： Morimoto T,Kanda H,Kondo M,Terasaki H,Nishida K,Fujikado T

更新日期：2012-06-28 00:00:00

abstract：PURPOSE:To study the topography of photoreceptor loss early in the course of Leber congenital amaurosis (LCA) caused by RPE65 mutations. METHODS:Young patients with RPE65-LCA (n = 9; ages, 6-17 years) were studied with optical coherence tomography (OCT) in a wide region of central retina. Outer nuclear layer (ONL) thi...

journal_title：Investigative ophthalmology & visual science

authors： Jacobson SG,Cideciyan AV,Aleman TS,Sumaroka A,Windsor EA,Schwartz SB,Heon E,Stone EM

更新日期：2008-10-01 00:00:00

abstract：PURPOSE:To identify the underlying mechanisms by which lipid mediator lysophosphatidic acid (LPA) acts as a growth factor in stimulating extracellular signal-regulated kinase (ERK) and phosphatidylinositol 3'-kinase (PI3K) during corneal epithelial wound healing. METHODS:Epithelial debridement wounds in cultured porci...

journal_title：Investigative ophthalmology & visual science

authors： Xu KP,Yin J,Yu FS

更新日期：2007-02-01 00:00:00

abstract：:The comparison of intracellular pH (pHi) regulation between fresh (FBCE) and cultured (CBCE) bovine corneal endothelium was extended to HCO3- transport mechanisms. Upon introduction of CO2/HCO3- Ringer's solution, there was a small, sharp acidification followed by an alkalinization .09 and .18 pH U above the HCO3- fre...

journal_title：Investigative ophthalmology & visual science

authors： Bonanno JA,Giasson C

更新日期：1992-10-01 00:00:00

abstract：PURPOSE:To create a binocular statistical eye model based on previously measured ocular biometric data. METHODS:Thirty-nine parameters were determined for a group of 127 healthy subjects (37 male, 90 female; 96.8% Caucasian) with an average age of 39.9 ± 12.2 years and spherical equivalent refraction of -0.98 ± 1.77 D...

journal_title：Investigative ophthalmology & visual science

authors： Rozema JJ,Atchison DA,Tassignon MJ

更新日期：2011-06-23 00:00:00

abstract：:A method for assessing the inward permeability of the blood-retinal barrier by fluorophotometry is presented. The permeability value is calculated with the fluorophotometer computer from fluorophotometric scan values in vitreous and non-protein-bound fluorescein concentration values in plasma. No diffusion coefficient...

journal_title：Investigative ophthalmology & visual science

authors： Kappelhof JP,van Best JA,van Valenberg PL,Oosterhuis JA

更新日期：1987-04-01 00:00:00

abstract：PURPOSE:We sought to determine whether conjunctival ultraviolet autofluorescence (UVAF), a biomarker of outdoor light exposure, is associated with myopia. METHODS:We performed a cross-sectional study on Norfolk Island and recruited individuals aged ≥ 15 years. Participants completed a sun-exposure questionnaire and un...

journal_title：Investigative ophthalmology & visual science

authors： Sherwin JC,Hewitt AW,Coroneo MT,Kearns LS,Griffiths LR,Mackey DA

更新日期：2012-07-01 00:00:00

abstract：Purpose:To refine the Minnesota Grading System (MGS) using definitions from the Age-Related Eye Disease Studies (AREDS) into a nine-step grading scale (MGS-9). Methods:A nine-step grading scale descriptive analysis using three key phenotypic features (total drusen area, increased, and decreased pigmentation) of human ...

journal_title：Investigative ophthalmology & visual science

authors： Olsen TW,Liao A,Robinson HS,Palejwala NV,Sprehe N

更新日期：2017-10-01 00:00:00

abstract：PURPOSE:Submucosal fibrosis due to excessive accumulation of collagens is an important histologic feature in the pathogenesis of ocular cicatricial pemphigoid (OCP). Heat shock protein 47 (HSP47), a collagen-binding protein, plays an important role in the biosynthesis of procollagens. In the present study, we examined ...

journal_title：Investigative ophthalmology & visual science

authors： Razzaque MS,Foster CS,Ahmed AR

更新日期：2003-04-01 00:00:00

abstract：PURPOSE:Corneal allografts placed in human eyes at high risk often fail, and immune rejection is thought to be a major pathogenic factor. To understand the immunologic factors responsible for rejection in this instance, the authors have created "high-risk" eyes in mice by inducing corneal neovascularization. The author...

journal_title：Investigative ophthalmology & visual science

authors： Sano Y,Ksander BR,Streilein JW

更新日期：1995-10-01 00:00:00

abstract：:Chronic blepharitis has been a difficult disease to define either microbiologically or biochemically. Sterols from meibomian secretions of normal subjects and patients were analyzed, and important differences were observed. Based on analyses of these secretions, two significantly different (P less than 0.001) types of...

journal_title：Investigative ophthalmology & visual science

authors： Shine WE,McCulley JP

更新日期：1991-07-01 00:00:00

abstract：PURPOSE:Ligands for aryl hydrocarbon receptor (AHR), such as dioxins, are highly toxic. One such ligand, TCDD, was found to exert potent immunosuppressive capacities in mice developing pathogenic autoimmune processes, including EAU, but its toxicity makes it unusable for humans. A recently identified endogenous AHR lig...

journal_title：Investigative ophthalmology & visual science

authors： Nugent LF,Shi G,Vistica BP,Ogbeifun O,Hinshaw SJ,Gery I

更新日期：2013-11-13 00:00:00