Molecular and phylogenetic characterization of the homoeologous EPSP Synthase genes of allohexaploid wheat, Triticum aestivum (L.).


BACKGROUND:5-Enolpyruvylshikimate-3-phosphate synthase (EPSPS) is the sixth and penultimate enzyme in the shikimate biosynthesis pathway, and is the target of the herbicide glyphosate. The EPSPS genes of allohexaploid wheat (Triticum aestivum, AABBDD) have not been well characterized. Herein, the three homoeologous copies of the allohexaploid wheat EPSPS gene were cloned and characterized. METHODS:Genomic and coding DNA sequences of EPSPS from the three related genomes of allohexaploid wheat were isolated using PCR and inverse PCR approaches from soft white spring "Louise'. Development of genome-specific primers allowed the mapping and expression analysis of TaEPSPS-7A1, TaEPSPS-7D1, and TaEPSPS-4A1 on chromosomes 7A, 7D, and 4A, respectively. Sequence alignments of cDNA sequences from wheat and wheat relatives served as a basis for phylogenetic analysis. RESULTS:The three genomic copies of wheat EPSPS differed by insertion/deletion and single nucleotide polymorphisms (SNPs), largely in intron sequences. RT-PCR analysis and cDNA cloning revealed that EPSPS is expressed from all three genomic copies. However, TaEPSPS-4A1 is expressed at much lower levels than TaEPSPS-7A1 and TaEPSPS-7D1 in wheat seedlings. Phylogenetic analysis of 1190-bp cDNA clones from wheat and wheat relatives revealed that: 1) TaEPSPS-7A1 is most similar to EPSPS from the tetraploid AB genome donor, T. turgidum (99.7 % identity); 2) TaEPSPS-7D1 most resembles EPSPS from the diploid D genome donor, Aegilops tauschii (100 % identity); and 3) TaEPSPS-4A1 resembles EPSPS from the diploid B genome relative, Ae. speltoides (97.7 % identity). Thus, EPSPS sequences in allohexaploid wheat are preserved from the most two recent ancestors. The wheat EPSPS genes are more closely related to Lolium multiflorum and Brachypodium distachyon than to Oryza sativa (rice). CONCLUSIONS:The three related EPSPS homoeologues of wheat exhibited conservation of the exon/intron structure and of coding region sequence, but contained significant sequence variation within intron regions. The genome-specific primers developed will enable future characterization of natural and induced variation in EPSPS sequence and expression. This can be useful in investigating new causes of glyphosate herbicide resistance.


BMC Genomics


BMC genomics


Aramrak A,Kidwell KK,Steber CM,Burke IC




Has Abstract


2015-10-23 00:00:00










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    authors: Fernández P,Paniego N,Lew S,Hopp HE,Heinz RA

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    abstract:BACKGROUND:Much of the morphological diversity in eukaryotes results from differential regulation of gene expression in which transcription factors (TFs) play a central role. The nematode Caenorhabditis elegans is an established model organism for the study of the roles of TFs in controlling the spatiotemporal pattern ...

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    pub_type: 杂志文章


    authors: Haerty W,Artieri C,Khezri N,Singh RS,Gupta BP

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    authors: Smagulova F,Brick K,Pu Y,Sengupta U,Camerini-Otero RD,Petukhova GV

    更新日期:2013-07-22 00:00:00

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    abstract:BACKGROUND:ATAC-seq (Assays for Transposase-Accessible Chromatin using sequencing) is a recently developed technique for genome-wide analysis of chromatin accessibility. Compared to earlier methods for assaying chromatin accessibility, ATAC-seq is faster and easier to perform, does not require cross-linking, has higher...

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    authors: Ou J,Liu H,Yu J,Kelliher MA,Castilla LH,Lawson ND,Zhu LJ

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    pub_type: 杂志文章


    authors: Eggleston H,Adelman ZN

    更新日期:2020-08-31 00:00:00

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    authors: Wanichnopparat W,Suwanwongse K,Pin-On P,Aporntewan C,Mutirangura A

    更新日期:2013-03-27 00:00:00

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    pub_type: 杂志文章


    authors: Nilsson MA,Janke A,Murchison EP,Ning Z,Hallström BM

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    journal_title:BMC genomics

    pub_type: 杂志文章


    authors: Huang Y,Linsen SE

    更新日期:2015-06-04 00:00:00

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    pub_type: 杂志文章


    authors: Apolis L,Olivas J,Srinivasan P,Kushwaha AK,Desai SA

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    authors: Sahl JW,Johnson JK,Harris AD,Phillippy AM,Hsiao WW,Thom KA,Rasko DA

    更新日期:2011-06-04 00:00:00