Analysis of the genome sequence of Phomopsis longicolla: a fungal pathogen causing Phomopsis seed decay in soybean.

Abstract:

BACKGROUND:Phomopsis longicolla T. W. Hobbs (syn. Diaporthe longicolla) is a seed-borne fungus causing Phomopsis seed decay in soybean. This disease is one of the most devastating diseases reducing soybean seed quality worldwide. To facilitate investigation of the genomic basis of pathogenicity and to understand the mechanism of the disease development, the genome of an isolate, MSPL10-6, from Mississippi, USA was sequenced, de novo assembled, and analyzed. RESULTS:The genome of MSPL 10-6 was estimated to be approximately 62 Mb in size with an overall G + C content of 48.6%. Of 16,597 predicted genes, 9866 genes (59.45%) had significant matches to genes in the NCBI nr database, while 18.01% of them did not link to any gene ontology classification, and 9.64% of genes did not significantly match any known genes. Analysis of the 1221 putative genes that encoded carbohydrate-activated enzymes (CAZys) indicated that 715 genes belong to three classes of CAZy that have a direct role in degrading plant cell walls. A novel fungal ulvan lyase (PL24; EC 4.2.2.-) was identified. Approximately 12.7% of the P. longicolla genome consists of repetitive elements. A total of 510 potentially horizontally transferred genes were identified. They appeared to originate from 22 other fungi, 26 eubacteria and 5 archaebacteria. CONCLUSIONS:The genome of the P. longicolla isolate MSPL10-6 represented the first reported genome sequence in the fungal Diaporthe-Phomopsis complex causing soybean diseases. The genome contained a number of Pfams not described previously. Information obtained from this study enhances our knowledge about this seed-borne pathogen and will facilitate further research on the genomic basis and pathogenicity mechanism of P. longicolla and aids in development of improved strategies for efficient management of Phomopsis seed decay in soybean.

journal_name

BMC Genomics

journal_title

BMC genomics

authors

Li S,Darwish O,Alkharouf NW,Musungu B,Matthews BF

doi

10.1186/s12864-017-4075-x

subject

Has Abstract

pub_date

2017-09-05 00:00:00

pages

688

issue

1

issn

1471-2164

pii

10.1186/s12864-017-4075-x

journal_volume

18

pub_type

杂志文章
  • The EH1 motif in metazoan transcription factors.

    abstract:BACKGROUND:The Engrailed Homology 1 (EH1) motif is a small region, believed to have evolved convergently in homeobox and forkhead containing proteins, that interacts with the Drosophila protein groucho (C. elegans unc-37, Human Transducin-like Enhancers of Split). The small size of the motif makes its reliable identifi...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-6-169

    authors: Copley RR

    更新日期:2005-11-27 00:00:00

  • Genome-wide SNP scan of pooled DNA reveals nonsense mutation in FGF20 in the scaleless line of featherless chickens.

    abstract:BACKGROUND:Scaleless (sc/sc) chickens carry a single recessive mutation that causes a lack of almost all body feathers, as well as foot scales and spurs, due to a failure of skin patterning during embryogenesis. This spontaneous mutant line, first described in the 1950s, has been used extensively to explore the tissue ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-13-257

    authors: Wells KL,Hadad Y,Ben-Avraham D,Hillel J,Cahaner A,Headon DJ

    更新日期:2012-06-19 00:00:00

  • Comprehensive analysis of genetic and evolutionary features of the hepatitis E virus.

    abstract:BACKGROUND:The hepatitis E virus (HEV) is the causative pathogen of hepatitis E, a global public health concern. HEV comprises 8 genotypes with a wide host range and geographic distribution. This study aims to determine the genetic factors influencing the molecular adaptive changes of HEV open reading frames (ORFs) and...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-019-6100-8

    authors: Baha S,Behloul N,Liu Z,Wei W,Shi R,Meng J

    更新日期:2019-10-29 00:00:00

  • Heterologous oligonucleotide microarrays for transcriptomics in a non-model species; a proof-of-concept study of drought stress in Musa.

    abstract:BACKGROUND:'Systems-wide' approaches such as microarray RNA-profiling are ideally suited to the study of the complex overlapping responses of plants to biotic and abiotic stresses. However, commercial microarrays are only available for a limited number of plant species and development costs are so substantial as to be ...

    journal_title:BMC genomics

    pub_type: 杂志文章,meta分析

    doi:10.1186/1471-2164-10-436

    authors: Davey MW,Graham NS,Vanholme B,Swennen R,May ST,Keulemans J

    更新日期:2009-09-16 00:00:00

  • Microsatellite development from genome skimming and transcriptome sequencing: comparison of strategies and lessons from frog species.

    abstract:BACKGROUND:Even though microsatellite loci frequently have been isolated using recently developed next-generation sequencing (NGS) techniques, this task is still difficult because of the subsequent polymorphism screening requires a substantial amount of time. Selecting appropriate polymorphic microsatellites is a criti...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-018-5329-y

    authors: Xia Y,Luo W,Yuan S,Zheng Y,Zeng X

    更新日期:2018-12-07 00:00:00

  • Analytical parameters and validation of homopolymer detection in a pyrosequencing-based next generation sequencing system.

    abstract:BACKGROUND:Current technologies in next-generation sequencing are offering high throughput reads at low costs, but still suffer from various sequencing errors. Although pyro- and ion semiconductor sequencing both have the advantage of delivering long and high quality reads, problems might occur when sequencing homopoly...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-018-4544-x

    authors: Ivády G,Madar L,Dzsudzsák E,Koczok K,Kappelmayer J,Krulisova V,Macek M Jr,Horváth A,Balogh I

    更新日期:2018-02-21 00:00:00

  • Flux of transcript patterns during soybean seed development.

    abstract:BACKGROUND:To understand gene expression networks leading to functional properties of the soybean seed, we have undertaken a detailed examination of soybean seed development during the stages of major accumulation of oils, proteins, and starches, as well as the desiccating and mature stages, using microarrays consistin...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-11-136

    authors: Jones SI,Gonzalez DO,Vodkin LO

    更新日期:2010-02-24 00:00:00

  • Inference of high resolution HLA types using genome-wide RNA or DNA sequencing reads.

    abstract:BACKGROUND:Accurate HLA typing at amino acid level (four-digit resolution) is critical in hematopoietic and organ transplantations, pathogenesis studies of autoimmune and infectious diseases, as well as the development of immunoncology therapies. With the rapid adoption of genome-wide sequencing in biomedical research,...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-15-325

    authors: Bai Y,Ni M,Cooper B,Wei Y,Fury W

    更新日期:2014-05-01 00:00:00

  • Transcriptomic analysis of flower induction for long-day pitaya by supplementary lighting in short-day winter season.

    abstract:BACKGROUND:Pitayas are currently attracting considerable interest as a tropical fruit with numerous health benefits. However, as a long-day plant, pitaya plants cannot flower in the winter season from November to April in Hainan, China. To harvest pitayas with high economic value in the winter season, it is necessary t...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-020-6726-6

    authors: Xiong R,Liu C,Xu M,Wei SS,Huang JQ,Tang H

    更新日期:2020-04-29 00:00:00

  • Multi-tissue transcriptomics of the black widow spider reveals expansions, co-options, and functional processes of the silk gland gene toolkit.

    abstract:BACKGROUND:Spiders (Order Araneae) are essential predators in every terrestrial ecosystem largely because they have evolved potent arsenals of silk and venom. Spider silks are high performance materials made almost entirely of proteins, and thus represent an ideal system for investigating genome level evolution of nove...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-15-365

    authors: Clarke TH,Garb JE,Hayashi CY,Haney RA,Lancaster AK,Corbett S,Ayoub NA

    更新日期:2014-05-23 00:00:00

  • Genome-wide binding of the orphan nuclear receptor TR4 suggests its general role in fundamental biological processes.

    abstract:BACKGROUND:The orphan nuclear receptor TR4 (human testicular receptor 4 or NR2C2) plays a pivotal role in a variety of biological and metabolic processes. With no known ligand and few known target genes, the mode of TR4 function was unclear. RESULTS:We report the first genome-wide identification and characterization o...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-11-689

    authors: O'Geen H,Lin YH,Xu X,Echipare L,Komashko VM,He D,Frietze S,Tanabe O,Shi L,Sartor MA,Engel JD,Farnham PJ

    更新日期:2010-12-02 00:00:00

  • Large-scale analysis of post-translational modifications in E. coli under glucose-limiting conditions.

    abstract:BACKGROUND:Post-translational modification (PTM) of proteins is central to many cellular processes across all domains of life, but despite decades of study and a wealth of genomic and proteomic data the biological function of many PTMs remains unknown. This is especially true for prokaryotic PTM systems, many of which ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-017-3676-8

    authors: Brown CW,Sridhara V,Boutz DR,Person MD,Marcotte EM,Barrick JE,Wilke CO

    更新日期:2017-04-17 00:00:00

  • Global gene expression profiling of brown to white adipose tissue transformation in sheep reveals novel transcriptional components linked to adipose remodeling.

    abstract:BACKGROUND:Large mammals are capable of thermoregulation shortly after birth due to the presence of brown adipose tissue (BAT). The majority of BAT disappears after birth and is replaced by white adipose tissue (WAT). RESULTS:We analyzed the postnatal transformation of adipose in sheep with a time course study of the ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-015-1405-8

    authors: Basse AL,Dixen K,Yadav R,Tygesen MP,Qvortrup K,Kristiansen K,Quistorff B,Gupta R,Wang J,Hansen JB

    更新日期:2015-03-19 00:00:00

  • Mobilization of retrotransposons as a cause of chromosomal diversification and rapid speciation: the case for the Antarctic teleost genus Trematomus.

    abstract:BACKGROUND:The importance of transposable elements (TEs) in the genomic remodeling and chromosomal rearrangements that accompany lineage diversification in vertebrates remains the subject of debate. The major impediment to understanding the roles of TEs in genome evolution is the lack of comparative and integrative ana...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-018-4714-x

    authors: Auvinet J,Graça P,Belkadi L,Petit L,Bonnivard E,Dettaï A,Detrich WH 3rd,Ozouf-Costaz C,Higuet D

    更新日期:2018-05-09 00:00:00

  • Paralog analyses reveal gene duplication events and genes under positive selection in Ixodes scapularis and other ixodid ticks.

    abstract:BACKGROUND:Hard ticks (family Ixodidae) are obligatory hematophagous ectoparasites of worldwide medical and veterinary importance. The haploid genomes of multiple species of ixodid ticks exceed 1 Gbp, prompting questions regarding gene, segmental and whole genome duplication in this phyletic group. The availability of ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-015-2350-2

    authors: Van Zee JP,Schlueter JA,Schlueter S,Dixon P,Sierra CA,Hill CA

    更新日期:2016-03-16 00:00:00

  • Differential gene expression and Hog1 interaction with osmoresponsive genes in the extremely halotolerant black yeast Hortaea werneckii.

    abstract:BACKGROUND:Fluctuations in external salinity force eukaryotic cells to respond by changes in the gene expression of proteins acting in protective biochemical processes, thus counteracting the changing osmotic pressure. The high-osmolarity glycerol (HOG) signaling pathway is essential for the efficient up-regulation of ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-8-280

    authors: Vaupotic T,Plemenitas A

    更新日期:2007-08-16 00:00:00

  • Single Cell Explorer, collaboration-driven tools to leverage large-scale single cell RNA-seq data.

    abstract:BACKGROUND:Single cell transcriptome sequencing has become an increasingly valuable technology for dissecting complex biology at a resolution impossible with bulk sequencing. However, the gap between the technical expertise required to effectively work with the resultant high dimensional data and the biological experti...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-019-6053-y

    authors: Feng D,Whitehurst CE,Shan D,Hill JD,Yue YG

    更新日期:2019-08-27 00:00:00

  • A genome-wide deletion mutant screen identifies pathways affected by nickel sulfate in Saccharomyces cerevisiae.

    abstract:BACKGROUND:The understanding of the biological function, regulation, and cellular interactions of the yeast genome and proteome, along with the high conservation in gene function found between yeast genes and their human homologues, has allowed for Saccharomyces cerevisiae to be used as a model organism to deduce biolo...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-10-524

    authors: Arita A,Zhou X,Ellen TP,Liu X,Bai J,Rooney JP,Kurtz A,Klein CB,Dai W,Begley TJ,Costa M

    更新日期:2009-11-15 00:00:00

  • Genomic characterization of ribitol teichoic acid synthesis in Staphylococcus aureus: genes, genomic organization and gene duplication.

    abstract:BACKGROUND:Staphylococcus aureus or MRSA (Methicillin Resistant S. aureus), is an acquired pathogen and the primary cause of nosocomial infections worldwide. In S. aureus, teichoic acid is an essential component of the cell wall, and its biosynthesis is not yet well characterized. Studies in Bacillus subtilis have disc...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-7-74

    authors: Qian Z,Yin Y,Zhang Y,Lu L,Li Y,Jiang Y

    更新日期:2006-04-05 00:00:00

  • RNA-seq analysis provides insights into cold stress responses of Xanthomonas citri pv. citri.

    abstract:BACKGROUND:Xanthomonas citri pv. citri (Xcc) is a citrus canker causing Gram-negative bacteria. Currently, little is known about the biological and molecular responses of Xcc to low temperatures. RESULTS:Results depicted that low temperature significantly reduced growth and increased biofilm formation and unsaturated ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-019-6193-0

    authors: Liao JX,Li KH,Wang JP,Deng JR,Liu QG,Chang CQ

    更新日期:2019-11-06 00:00:00

  • Syntenic relationships between cucumber (Cucumis sativus L.) and melon (C. melo L.) chromosomes as revealed by comparative genetic mapping.

    abstract:BACKGROUND:Cucumber, Cucumis sativus L. (2n = 2 × = 14) and melon, C. melo L. (2n = 2 × = 24) are two important vegetable species in the genus Cucumis (family Cucurbitaceae). Both species have an Asian origin that diverged approximately nine million years ago. Cucumber is believed to have evolved from melon through chr...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-12-396

    authors: Li D,Cuevas HE,Yang L,Li Y,Garcia-Mas J,Zalapa J,Staub JE,Luan F,Reddy U,He X,Gong Z,Weng Y

    更新日期:2011-08-05 00:00:00

  • Genes related to the very early stage of ConA-induced fulminant hepatitis: a gene-chip-based study in a mouse model.

    abstract:BACKGROUND:Due to the high morbidity and mortality of fulminant hepatitis, early diagnosis followed by early effective treatment is the key for prognosis improvement. So far, little is known about the gene expression changes in the early stage of this serious illness. Identification of the genes related to the very ear...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-11-240

    authors: Chen F,Zhu HH,Zhou LF,Li J,Zhao LY,Wu SS,Wang J,Liu W,Chen Z

    更新日期:2010-04-15 00:00:00

  • Transcript profiling of Populus tomentosa genes in normal, tension, and opposite wood by RNA-seq.

    abstract:BACKGROUND:Wood formation affects the chemical and physical properties of wood, and thus affects its utility as a building material or a feedstock for biofuels, pulp and paper. To obtain genome-wide insights on the transcriptome changes and regulatory networks in wood formation, we used high-throughput RNA sequencing t...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-015-1390-y

    authors: Chen J,Chen B,Zhang D

    更新日期:2015-03-10 00:00:00

  • Patterns of gene expression during Arabidopsis flower development from the time of initiation to maturation.

    abstract:BACKGROUND:The formation of flowers is one of the main model systems to elucidate the molecular mechanisms that control developmental processes in plants. Although several studies have explored gene expression during flower development in the model plant Arabidopsis thaliana on a genome-wide scale, a continuous series ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-015-1699-6

    authors: Ryan PT,Ó'Maoiléidigh DS,Drost HG,Kwaśniewska K,Gabel A,Grosse I,Graciet E,Quint M,Wellmer F

    更新日期:2015-07-01 00:00:00

  • Comparative analysis of expression of histone H2a genes in mouse.

    abstract:BACKGROUND:At least 18 replication-dependent histone H2a genes are distributed in 3 Hist gene clusters on different chromosomes of the mouse genome. In this analysis we designed specific PCR primers for each histone H2a transcript and studied the expression levels and patterns using quantitative RT-PCR (qRT-PCR). In ad...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-6-108

    authors: Nishida H,Suzuki T,Ookawa H,Tomaru Y,Hayashizaki Y

    更新日期:2005-08-13 00:00:00

  • SeAMotE: a method for high-throughput motif discovery in nucleic acid sequences.

    abstract:BACKGROUND:The large amount of data produced by high-throughput sequencing poses new computational challenges. In the last decade, several tools have been developed for the identification of transcription and splicing factor binding sites. RESULTS:Here, we introduce the SeAMotE (Sequence Analysis of Motifs Enrichment)...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-15-925

    authors: Agostini F,Cirillo D,Ponti RD,Tartaglia GG

    更新日期:2014-10-23 00:00:00

  • A bacterial artificial chromosome library for the Australian saltwater crocodile (Crocodylus porosus) and its utilization in gene isolation and genome characterization.

    abstract:BACKGROUND:Crocodilians (Order Crocodylia) are an ancient vertebrate group of tremendous ecological, social, and evolutionary importance. They are the only extant reptilian members of Archosauria, a monophyletic group that also includes birds, dinosaurs, and pterosaurs. Consequently, crocodilian genomes represent a gat...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-10-S2-S9

    authors: Shan X,Ray DA,Bunge JA,Peterson DG

    更新日期:2009-07-14 00:00:00

  • Altering gene expression by aminocoumarins: the role of DNA supercoiling in Staphylococcus aureus.

    abstract:BACKGROUND:It has been shown previously that aminocoumarin antibiotics such as novobiocin lead to immediate downregulation of recA expression and thereby inhibit the SOS response, mutation frequency and recombination capacity in Staphylococcus aureus. Aminocoumarins function by inhibiting the ATPase activity of DNA gyr...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-15-291

    authors: Schröder W,Bernhardt J,Marincola G,Klein-Hitpass L,Herbig A,Krupp G,Nieselt K,Wolz C

    更新日期:2014-04-16 00:00:00

  • A potential role for intragenic miRNAs on their hosts' interactome.

    abstract:BACKGROUND:miRNAs are small, non-coding RNA molecules that mainly act as negative regulators of target gene messages. Due to their regulatory functions, they have lately been implicated in several diseases, including malignancies. Roughly half of known miRNA genes are located within previously annotated protein-coding ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-11-533

    authors: Hinske LC,Galante PA,Kuo WP,Ohno-Machado L

    更新日期:2010-10-01 00:00:00

  • Genomic and systems evolution in Vibrionaceae species.

    abstract:BACKGROUND:The steadily increasing number of prokaryotic genomes has accelerated the study of genome evolution; in particular, the availability of sets of genomes from closely related bacteria has facilitated the exploration of the mechanisms underlying genome plasticity. The family Vibrionaceae is found in the Gammapr...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-10-S1-S11

    authors: Gu J,Neary J,Cai H,Moshfeghian A,Rodriguez SA,Lilburn TG,Wang Y

    更新日期:2009-07-07 00:00:00