当前位置： SCI文献检索 > CANCER BIOLOGY & THERAPY期刊下所有文献 > Chronic exposure to chewing tobacco selects for overexpression of stearoyl-CoA desaturase in normal oral keratinocytes.

Chronic exposure to chewing tobacco selects for overexpression of stearoyl-CoA desaturase in normal oral keratinocytes.

Abstract：

:Chewing tobacco is a common practice in certain socio-economic sections of southern Asia, particularly in the Indian subcontinent and has been well associated with head and neck squamous cell carcinoma. The molecular mechanisms of chewing tobacco which leads to malignancy remains unclear. In large majority of studies, short-term exposure to tobacco has been evaluated. From a biological perspective, however, long-term (chronic) exposure to tobacco mimics the pathogenesis of oral cancer more closely. We developed a cell line model to investigate the chronic effects of chewing tobacco. Chronic exposure to tobacco resulted in higher cellular proliferation and invasive ability of the normal oral keratinocytes (OKF6/TERT1). We carried out quantitative proteomic analysis of OKF6/TERT1 cells chronically treated with chewing tobacco compared to the untreated cells. We identified a total of 3,636 proteins among which expression of 408 proteins were found to be significantly altered. Among the overexpressed proteins, stearoyl-CoA desaturase (SCD) was found to be 2.6-fold overexpressed in the tobacco treated cells. Silencing/inhibition of SCD using its specific siRNA or inhibitor led to a decrease in cellular proliferation, invasion and colony forming ability of not only the tobacco treated cells but also in a panel of head and neck cancer cell lines. These findings suggest that chronic exposure to chewing tobacco induced carcinogenesis in non-malignant oral epithelial cells and SCD plays an essential role in this process. The current study provides evidence that SCD can act as a potential therapeutic target in head and neck squamous cell carcinoma, especially in patients who are users of tobacco.

journal_name

Cancer Biol Ther

journal_title

Cancer biology & therapy

authors

Nanjappa V,Renuse S,Sathe GJ,Raja R,Syed N,Radhakrishnan A,Subbannayya T,Patil A,Marimuthu A,Sahasrabuddhe NA,Guerrero-Preston R,Somani BL,Nair B,Kundu GC,Prasad TK,Califano JA,Gowda H,Sidransky D,Pandey A,Chatterje

doi

10.1080/15384047.2015.1078022

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

1593-603

issue

eissn

1538-4047

issn

1555-8576

journal_volume

pub_type

杂志文章

在线工具

Chronic exposure to chewing tobacco selects for overexpression of stearoyl-CoA desaturase in normal oral keratinocytes.