Abstract:
:The rice leaf roller (Cnaphalocrocis medinalis) is one of the most destructive agricultural pests. Due to its migratory behavior, it is difficult to control worldwide. To date, little is known about major genes of C. medinalis involved in chitin metabolism and insecticide detoxification. In order to obtain a comprehensive genome dataset of C. medinalis, we conducted de novo transcriptome sequencing which focused on the major feeding stage of fourth-instar larvae, and our work revealed useful information on chitin metabolism and insecticide detoxification and target genes of C. medinalis. We acquired 29,367,797 Illumina reads and assembled these reads into 63,174 unigenes with an average length of 753 bp. Among these unigenes, 31,810 were annotated against the National Center for Biotechnology Information non-redundant (NCBI nr) protein database, resulting in 24,246, 8669 and 18,176 assigned to Swiss-Prot, clusters of orthologous group (COG), and gene ontology (GO), respectively. We were able to map 10,043 unigenes into 285 pathways using the Kyoto Encyclopedia of Genes and Genomes Pathway database (KEGG). Specifically, 16 genes, including five chitin deacetylases, two chitin synthases, five chitinases and four other related enzymes, were identified to be putatively involved in chitin biosynthesis and degradation, whereas 360 genes, including cytochrome P450s, glutathione S-transferases, esterases, and acetylcholinesterases, were found to be potentially involved in insecticide detoxification or as insecticide targets. The reliability of the transcriptome data was determined by reverse transcription quantitative PCR (RT-qPCR) for the selected genes. Our data serves as a new and valuable sequence resource for genomic studies on C. medinalis. The findings should improve our understanding of C. medinalis genetics and contribute to management of this important agricultural pest.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Yu HZ,Wen DF,Wang WL,Geng L,Zhang Y,Xu JPdoi
10.3390/ijms160921873subject
Has Abstractpub_date
2015-09-10 00:00:00pages
21873-96issue
9issn
1422-0067pii
ijms160921873journal_volume
16pub_type
杂志文章abstract::Vascular calcification, which involves the deposition of calcifying particles within the arterial wall, is mediated by atherosclerosis, vascular smooth muscle cell osteoblastic changes, adventitial mesenchymal stem cell osteoblastic differentiation, and insufficiency of the calcification inhibitors. Recent observation...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21072466
更新日期:2020-04-02 00:00:00
abstract::Radiolysis of biomolecules by fast ions has interest in medical applications and astrobiology. The radiolysis of solid D-valine (0.2-2 μm thick) was performed at room temperature by 1.5 MeV H+, He+, N+, and 230 MeV S15+ ion beams. The samples were prepared by spraying/dropping valine-water-ethanol solution on ZnSe sub...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21051893
更新日期:2020-03-10 00:00:00
abstract::Opioid analgesics such as morphine have indispensable roles in analgesia. However, morphine use can elicit side effects such as respiratory depression and constipation. It has been reported that G protein-biased agonists as substitutes for classic opioid agonists can alleviate (or even eliminate) these side effects. T...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21134699
更新日期:2020-07-01 00:00:00
abstract::Targeting adenosine triphosphate (ATP) metabolism and adenosinergic signaling in cancer is gaining momentum, as increasing evidence is showing their relevance in tumor immunology and biology. Chronic lymphocytic leukemia (CLL) results from the expansion of a population of mature B cells that progressively occupies the...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19041167
更新日期:2018-04-12 00:00:00
abstract::Cannabidiol (CBD) is known for its vasorelaxant (including in the human pulmonary artery), anti-proliferative and anti-inflammatory properties. The aim of our study was to examine the potential preventive effect of chronic CBD administration (10 mg/kg/day for three weeks) on monocrotaline (MCT)-induced pulmonary hyper...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21197077
更新日期:2020-09-25 00:00:00
abstract::While necroptosis has been shown to contribute to the pathogenesis of post-infarction heart failure (HF), the role of autophagy remains unclear. Likewise, linkage between these two cell death modalities has not been sufficiently investigated. HF was induced by 60-min left coronary occlusion in adult Wistar rats and he...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21207782
更新日期:2020-10-21 00:00:00
abstract::Nanotechnology has rapidly promoted the development of a new generation of industrial and commercial products; however, it has also raised some concerns about human health and safety. To evaluate the toxicity of the great diversity of nanomaterials (NMs) in the traditional manner, a tremendous number of safety assessm...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21072387
更新日期:2020-03-30 00:00:00
abstract::Myotonic dystrophy type 1 (DM1) is a complex neuromuscular disease caused by an unstable cardiotocography (CTG) repeat expansion in the DMPK gene. This disease is characterized by high clinical and genetic variability, leading to some difficulties in the diagnosis and prognosis of DM1. Better understanding the origin ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21020457
更新日期:2020-01-10 00:00:00
abstract::The silkworm is an oligophagous insect for which mulberry leaves are the sole diet. The nutrients needed for vital activities of the egg, pupal, and adult stages, and the proteins formed in the cocoon, are all derived from the larval stages. The silkworm feeds and grows quickly during the larval stages. In particular,...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20246113
更新日期:2019-12-04 00:00:00
abstract::In this study, new composite bisphosphonate delivery systems were obtained from polyurethanes (PUs) and nanocrystalline hydroxyapatite (HA). The biodegradable PUs were first synthesized from poly(ε-caprolactone) diols (PCL diols), poly(ethylene adipate) diol, 1,6-hexamethylene diisocyanate, 1,4-butanediol and HA. More...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms150916831
更新日期:2014-09-22 00:00:00
abstract::The basal forebrain is home to the largest population of cholinergic neurons in the brain. These neurons are involved in a number of cognitive functions including attention, learning and memory. Basal forebrain cholinergic neurons (BFCNs) are particularly vulnerable in a number of neurological diseases with the most n...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17122122
更新日期:2016-12-17 00:00:00
abstract::Chaetomium globosum is frequently isolated in water-damaged buildings and produces two mycotoxins called chaetoglobosins A and C when cultured on building material. In this study, the influence of ambient pH on the growth of C. globosum was examined on an artificial medium. This fungus was capable of growth on potato ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms9122357
更新日期:2008-12-01 00:00:00
abstract::It was reported that substance P had beneficial effects in the healing of acute tendon injury. However, the relationship between substance P and degenerative tendinopathy development remains unclear. The purpose of this study was to determine the role of substance P in the pathogenesis of tendinopathy. Healthy and ten...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18061241
更新日期:2017-06-09 00:00:00
abstract::The regeneration of neurons is an important goal of neuroscience and clinical medicine. The electrical stimulation of cells is a promising technique to meet this goal. However, its efficiency highly depends on the electrochemical properties of the stimulation electrodes used. This work reports on the preparation and u...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20082013
更新日期:2019-04-24 00:00:00
abstract::The variable nature of vascular dysfunction in diabetes is not well understood. We explored the functional adaptation of different arteries in db/db mice in relation to increased severity and duration of diabetes. We compared endothelium-dependent and -independent vasodilation in the aortae, as well as the carotid and...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21176147
更新日期:2020-08-26 00:00:00
abstract::Microalgae are unicellular photosynthetic organisms considered as potential alternative sources for biomass, biofuels or high value products. However, limited biomass productivity is commonly experienced in their cultivating system despite their high potential. One of the reasons for this limitation is the high therma...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19010155
更新日期:2018-01-05 00:00:00
abstract::In plants, microRNAs have been indicated to control various developmental processes, including somatic embryogenesis (SE), which is triggered in the in vitro cultured somatic cells of plants. Although a transcriptomic analysis has indicated that numerous MIRNAs are differentially expressed in the SE of different plant...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20205221
更新日期:2019-10-21 00:00:00
abstract::Due to the central role of liver tissue in partitioning and metabolizing of nutrients, molecular liver-specific alterations are of considerable interest to characterize an efficient conversion and usage of feed in livestock. To deduce tissue-specific and systemic effects on nutrient metabolism and feed efficiency (FE)...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18081674
更新日期:2017-08-01 00:00:00
abstract::Phosphate (Pi) is a pivotal nutrient that constraints plant development and productivity in natural ecosystems. Land colonization by plants, more than 470 million years ago, evolved adaptive mechanisms to conquer Pi-scarce environments. However, little is known about the molecular basis underlying such adaptations at ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21218354
更新日期:2020-11-07 00:00:00
abstract::In this study, a surface chemical-modified rice husk biochar with abundant amino groups and disulfide bonds for the removal of cadmium was prepared using cystamine dihydrochloride as a modification ligand and glutaraldehyde as a crosslinker. The biochars were characterized by Fourier transform infrared spectrometry (F...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20071775
更新日期:2019-04-10 00:00:00
abstract::One of the major goals of the Chromosome-Centric Human Proteome Project (C-HPP) is to catalog and annotate a myriad of heterogeneous proteoforms, produced by ca. 20 thousand genes. To achieve a detailed and personalized understanding into proteomes, we suggest using a customized RNA-seq library of potential proteoform...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21113813
更新日期:2020-05-27 00:00:00
abstract::Both positive and negative aspects of sport performance are currently considered. The aim of our study was to determine time- and intensity-dependent effects of a single exercise bout on redox and inflammatory status. The experiment was performed on 40 male Wistar rats subjected to treadmill running for 30 min with th...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21218071
更新日期:2020-10-29 00:00:00
abstract::Oral squamous cell carcinoma (OSCC) is one of the most common cancers worldwide. In China, its 5-year survival rate is roughly 50%, owing to acquired chemotherapeutic resistance and metastasis of the disease. Accumulating evidence demonstrates that aspirin (ASA) acts as a preventive or therapeutic agent in multiple ca...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19072029
更新日期:2018-07-12 00:00:00
abstract::Acidovorax citrulli (A. citrulli) strains cause bacterial fruit blotch (BFB) in cucurbit crops and affect melon significantly. Numerous strains of the bacterium have been isolated from melon hosts globally. Strains that are aggressively virulent towards melon and diagnostic markers for detecting such strains are yet t...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20112715
更新日期:2019-06-02 00:00:00
abstract::Extracellular vesicles (EVs) are a heterogeneous group of particles, between 15 nanometers and 10 microns in diameter, released by almost all cell types in physiological and pathological conditions, including tumors. EVs have recently emerged as particularly interesting informative vehicles, so that they could be cons...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18061183
更新日期:2017-06-02 00:00:00
abstract::Advances in the use of targeted and immune therapies have revolutionized the clinical management of melanoma patients, prolonging significantly their overall and progression-free survival. However, both targeted and immune therapies suffer limitations due to genetic mutations and epigenetic modifications, which determ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21124544
更新日期:2020-06-26 00:00:00
abstract::Ergosterol peroxide is a natural compound of the steroid family found in many fungi, and it possesses antioxidant, anti-inflammatory, anticancer and antiviral activities. The anti-obesity activity of several edible and medicinal mushrooms has been reported, but the effect of mushroom-derived ergosterol peroxide on obe...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21020460
更新日期:2020-01-10 00:00:00
abstract::Polymer supported dichlorophosphate (PEG-OPOCl(2)) is an efficient green catalyst for the electrophilic substitution reaction of indole with aromatic aldehydes, in neat condition, to afford an excellent yield of bis(indolyl) methanes with short reaction time, at room temperature. The synthesized compounds and their an...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms14011843
更新日期:2013-01-16 00:00:00
abstract:BACKGROUND:Fisetin (3,7,3',4'-tetrahydroxyflavone) is a dietary flavonol and exhibits antioxidant, anti-inflammatory, and neuroprotective activities. However, high concentration of fisetin is reported to produce reactive oxygen species (ROS), induce endoplasmic reticulum (ER) stress and cause cytotoxicity in cancer cel...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18040852
更新日期:2017-04-17 00:00:00
abstract::Aging is an inevitable and gradually progressive process affecting all organs and systems. The musculoskeletal system makes no exception, elderly exhibit an increased risk of sarcopenia (low muscle mass),dynapenia (declining muscle strength), and subsequent disability. Whereas in recent years the subject of skeletal m...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21020592
更新日期:2020-01-16 00:00:00