Abstract:
:One of the major goals of the Chromosome-Centric Human Proteome Project (C-HPP) is to catalog and annotate a myriad of heterogeneous proteoforms, produced by ca. 20 thousand genes. To achieve a detailed and personalized understanding into proteomes, we suggest using a customized RNA-seq library of potential proteoforms, which includes aberrant variants specific to certain biological samples. Two-dimensional electrophoresis coupled with high-performance liquid chromatography allowed us to downgrade the difficulty of biological mixing following shotgun mass spectrometry. To benchmark the proposed pipeline, we examined heterogeneity of the HepG2 hepatoblastoma cell line proteome. Data are available via ProteomeXchange with identifier PXD018450.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Kiseleva O,Ponomarenko E,Poverennaya Edoi
10.3390/ijms21113813subject
Has Abstractpub_date
2020-05-27 00:00:00issue
11issn
1422-0067pii
ijms21113813journal_volume
21pub_type
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