Abstract:
:Chemical probes are small molecules designed to bind to a specific protein and disrupt the proteins function. Although many inhibitors are reported for human carbonic anhydrase (CA) enzymes, few may be considered useful as chemical probes as they exhibit broad action against the 12 catalytically active CA isozymes. In addition, most do not possess an appropriate physicochemical profile to discriminate intracellular CA activity from either global or extracellular CA activity. We report herein the synthesis of three monophosphate CA proinhibitors (compounds 2, 3, and 5) that are derived from cyclosaligenyl (cycloSal) phosphate and S-acyl-2-thioethyl (SATE) phosphate as protecting groups. The proinhibitors are designed as neutral, membrane-permeable compounds that once inside the cell may be hydrolyzed by pH-driven or enzymatic-driven mechanisms to release a negatively charged monophosphate. The resulting monophosphate compound is trapped intracellularly and available for locality specific inhibition of intracellular CAs.
journal_name
J Med Chemjournal_title
Journal of medicinal chemistryauthors
Rankin GM,Vullo D,Supuran CT,Poulsen SAdoi
10.1021/acs.jmedchem.5b01228subject
Has Abstractpub_date
2015-09-24 00:00:00pages
7580-90issue
18eissn
0022-2623issn
1520-4804journal_volume
58pub_type
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