Abstract：
:Europium (Eu(3+)) and samarium (Sm(3+)) were used as fluorescent labels to develop a highly sensitive dual-labeled time-resolved fluoroimmunoassay (TRFIA) for detect clothianidin and diniconazole in food samples. Under the optimized assay conditions, 50% inhibition concentration (IC50) and the limit of detection (LOD, IC10) of clothianidin were 5.08 and 0.021 μg/L, and 13.14 and 0.029 μg/L for diniconazole. The cross-reactivities (CRs) were negligible except dinotefuran (9.4%) and uniconazole (4.28%). The recoveries of clothianidin and diniconazole ranged from 79.3% to 108.7% in food samples. The results of TRFIA for the authentic samples were validated by gas chromatography (GC) analyses, and a satisfactory correlations were obtained. These results indicated that the method was an alternative tool for simultaneous detection of clothianidin and diniconazole in food samples.
journal_name
Food Chemjournal_title
Food chemistryauthors
Sheng E,Shi H,Zhou L,Hua X,Feng L,Yu T,Wang Mdoi
10.1016/j.foodchem.2015.07.023subject
Has Abstractpub_date
2016-02-01 00:00:00pages
525-30eissn
0308-8146issn
1873-7072pii
S0308-8146(15)01028-6journal_volume
192pub_type
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