Blockade of CXCR6 reduces invasive potential of gastric cancer cells through inhibition of AKT signaling.

Abstract:

:Chemokines and their receptors have been implicated in cell migration and metastasis of multiple malignant tumors. But the function of CXCR6 signaling in gastric cancer is not comprehensively understood. In the present study, we hypothesized that CXCR6 signaling might play an essential role in the progression of gastric cancer. The expression of CXCR6 was examined by immunohistochemical assay in human gastric cancer, and lentivirus-mediated CXCR6 knockdown by shRNA (Lv-shCXCR6) was used for investigating cell migration and invasion indicated by Wound-healing and Transwell assays. Consequently, the expression level of CXCR6 was increased in gastric cancer compared with the adjacent non-tumor tissues (54.2% vs. 27.1%, P = 0.006), and was closely associated with the metastatic lymph node in gastric cancer (P = 0.021). Furthermore, blockade of the CXCR6 signaling reduced the migration and invasion of gastric cancer cells followed by decreased expression of AKT, MMP-2, and MMP-9. In conclusion, these findings demonstrate that CXCR6 may promote the development of gastric cancer cells through regulation of AKT signaling.

authors

Li Y,Fu LX,Zhu WL,Shi H,Chen LJ,Ye B

doi

10.1177/0394632015584502

subject

Has Abstract

pub_date

2015-06-01 00:00:00

pages

194-200

issue

2

eissn

0394-6320

issn

2058-7384

pii

0394632015584502

journal_volume

28

pub_type

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