Abstract:
:A human liver cDNA library was screened by colony hybridization with a bovine factor X cDNA probe. Three of the positive plasmids contained overlapping DNA that coded for most of human factor X mRNA. DNA sequence analysis of these three clones allowed the prediction of the complete amino acid sequence of plasma factor X. From these studies, we predict that human factor X is synthesized as a single polypeptide chain precursor in which the light and heavy chains of plasma factor X are linked by the tripeptide Arg-Lys-Arg. The cDNA sequence also predicts that human factor X is synthesized as a preproprotein having an amino-terminal leader peptide of at least 28 amino acid residues. A comparison of the amino acid sequences of human and bovine factor X shows high sequence identity around the calcium-binding regions and catalytic regions but low sequence identity around the nonfunctional regions.
journal_name
Proc Natl Acad Sci U S Aauthors
Fung MR,Hay CW,MacGillivray RTdoi
10.1073/pnas.82.11.3591subject
Has Abstractpub_date
1985-06-01 00:00:00pages
3591-5issue
11eissn
0027-8424issn
1091-6490journal_volume
82pub_type
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