The MAT1-1:MAT1-2 ratio of Sporothrix globosa isolates in Japan.

Abstract:

:In order to understand the reproductive biology of pathogenic species in the Sporothrix schenckii complex, we characterized the partial mating type (MAT1-1) loci of Sporothrix schenckii, as well as the S. globosa MAT1-1-1 gene, which encoded 262 amino acid sequences. The data confirmed that the MAT1-1 locus of S. globosa was divergent from the MAT1-2 locus of the opposite mating type, suggesting that the fungus is heterothallic. To determine the mating type ratio of 20 isolates from Japanese patients, we analyzed the MAT loci by specific PCR amplification of MAT1-1-1 and MAT1-2-1 genes. The MAT1-1-1 was detected in 5 isolates but not in the other 15 isolates with the presence of MAT1-2-1. The MAT1-1:1-2 ratio of S. globosa isolates in Japan was estimated to be 1:3. Phylogenetic analysis indicated that the sequences of the MAT1-1-1 were identical among S. globosa isolates but different from S. schenckii and Ophiostoma montium.

journal_name

Mycopathologia

journal_title

Mycopathologia

authors

Kano R,Tsui CK,Hamelin RC,Anzawa K,Mochizuki T,Nishimoto K,Hiruma M,Kamata H,Hasegawa A

doi

10.1007/s11046-014-9808-7

subject

Has Abstract

pub_date

2015-02-01 00:00:00

pages

81-6

issue

1-2

eissn

0301-486X

issn

1573-0832

journal_volume

179

pub_type

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