Abstract：
:In this study, four different haptens around the oxime moiety of Butocarboxim were designed and synthesised. Two of the haptens were conjugated with bovine serum albumin (BSA) to serve as the immunogen and all the haptens were conjugated with ovalbumin (OVA) for the coating antigen. The anti-Butocarboxim monoclonal antibody (Mab) was selected based on eight immunogen/coating antigen combinations. The first enzyme-linked chemiluminescent immunoassay (ELCIA) for determining Butocarboxim in agricultural products was developed. Under the optimised conditions, the detection limit for the ELCIA was 20 ng·mL(-1) and the linear range was 27-2700 ng·mL(-1). Analyte recoveries for extracts of spiked agricultural (apple and greengrocery) products and tap water ranged from 97.18% to 107.00%. The developed immunoassay has great potential to be developed as a test kit offering a simple and cost-effective approach (such as lateral flow test strip) for screening purposes and evaluating environmental exposure to Butocarboxim.
journal_name
Food Chemjournal_title
Food chemistryauthors
Fang Q,Wang L,Hua X,Wang Y,Wang S,Cheng Q,Cai J,Liu Fdoi
10.1016/j.foodchem.2014.06.060subject
Has Abstractpub_date
2015-01-01 00:00:00pages
372-379eissn
0308-8146issn
1873-7072pii
S0308-8146(14)00946-7journal_volume
166pub_type
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