Abstract：
:Ultra performance liquid chromatography coupled to mass spectrometry was used for the simultaneous separation and determination of reducing monosaccharides (fructose and glucose), a non-reducing disaccharide (sucrose) and oligosaccharides (kestose and nystose) in HILIC mode. The chromatographic separation of all saccharides was performed on a BEH amide column using an acetonitrile-water gradient elution. The detection was carried out using selected ion recording (SIR) acquisition mode. The validation of the proposed method showed that the limit of detection and limit of quantification values for the five analyzed compounds were in the range of 0.25-0.69μg/mL and 0.82-3.58μg/mL, respectively; while the response was linear in the range of 1-50μg/mL. The developed method showed potential usefulness for a rapid and sensitive analysis of underivatized saccharides and was used for determination of sugars in three date samples (Sefri, Mabroom, Ghassab) which were soxhlet extracted by ethanol.
journal_name
Food Chemjournal_title
Food chemistryauthors
Ghfar AA,Wabaidur SM,Ahmed AY,Alothman ZA,Khan MR,Al-Shaalan NHdoi
10.1016/j.foodchem.2014.12.035subject
Has Abstractpub_date
2015-06-01 00:00:00pages
487-92eissn
0308-8146issn
1873-7072pii
S0308-8146(14)01946-3journal_volume
176pub_type
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