Abstract：
:Channel catfish skin is a by-product from catfish fillet production. Collagens were extracted from catfish skins by: (1) acid; (2) homogenization-aided; and (3) pepsin-aided extraction methods. Kinetic analysis of extraction was performed. SDS-PAGE was carried out for all collagens extracted under different conditions. Protein solubility, zeta potential, circular dichroism and gel strength of the collagen extracted by three methods were studied to determine optimal extraction conditions. Protein recovery rate from minced skins extracted with pH 2.4 HCl containing 23.6KU/g pepsin was the highest (64.19%). SDS-PAGE showed that collagens extracted with different methods had different proteins ratio patterns, even though the molecular mass of collagen subunits were similar, 123 and 113KDa for α1 and α2 chains, 226KDa for β chain and 338.5KDa for γ chain, respectively. Channel catfish skin collagens were typical type I collagens and could have applications in food, medical and cosmetic industries.
journal_name
Food Chemjournal_title
Food chemistryauthors
Tan Y,Chang SKCdoi
10.1016/j.foodchem.2017.09.013subject
Has Abstractpub_date
2018-03-01 00:00:00pages
147-155eissn
0308-8146issn
1873-7072pii
S0308-8146(17)31472-3journal_volume
242pub_type
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