Abstract：
:Hepatic transporters and metabolic enzymes affect drug pharmacokinetics. Limited information exists on the alteration in mRNA levels of hepatic transporters and metabolic enzymes with aging. We examined the effects of aging on the mRNA levels of representative hepatic drug transporters and metabolic enzymes by analyzing their levels in 10-, 30- and 50-week-old male and female rats. Levels of mRNA of drug transporters including multidrug resistance protein (Mdr)1a, multidrug resistance-associated protein (Mrp)2, breast cancer resistance protein (Bcrp) and organic anion-transporting polypeptide (Oatp)1a1, and the metabolic enzymes cytochrome P450 (CYP)3A1, CYP3A2 and UDP-glucuronosyltransferase (UGT)1A1 were analyzed using real-time reverse transcriptase polymerase chain reaction. The mRNA levels of transporters in male rats did not decrease with age, while the mRNA levels of Bcrp and Oatp1a1 in female rats decreased with age. The mRNA levels of CYP3A1 and CYP3A2 in male rats were higher than those in female rats. The mRNA levels of metabolic enzymes decreased with age in female but not male rats. In particular, the mRNA levels of UGT1A1 in 10-week-old female rats were higher than those in male rats. mRNA expression of hepatic transporters and metabolic enzymes are more susceptible to aging in female than male rats. The age-related decreases in the mRNA levels of Bcrp, Oatp1a1, CYP3A1 and CYP3A2 in female rats may affect the metabolism and transport of substrates. This study showed that aging affected the mRNA expression of hepatic transporters and metabolic enzymes in rats.
journal_name
Eur J Drug Metab Pharmacokinetjournal_title
European journal of drug metabolism and pharmacokineticsauthors
Kawase A,Ito A,Yamada A,Iwaki Mdoi
10.1007/s13318-014-0208-7subject
Has Abstractpub_date
2015-06-01 00:00:00pages
239-44issue
2eissn
0378-7966issn
2107-0180journal_volume
40pub_type
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journal_title：European journal of drug metabolism and pharmacokinetics
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journal_title：European journal of drug metabolism and pharmacokinetics
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journal_title：European journal of drug metabolism and pharmacokinetics
pub_type： 杂志文章,评审
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journal_title：European journal of drug metabolism and pharmacokinetics
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更新日期：1995-04-01 00:00:00
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journal_title：European journal of drug metabolism and pharmacokinetics
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更新日期：1980-01-01 00:00:00
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journal_title：European journal of drug metabolism and pharmacokinetics
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更新日期：1994-10-01 00:00:00
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更新日期：1991-01-01 00:00:00
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