Abstract:
:Cell death-inducing DFF45-like effector b (Cideb), an endoplasmic reticulum (ER)- and lipid droplet (LD)-associated protein, has been shown to play a critical role in maintaining hepatic lipid homeostasis by promoting the lipidation and maturation of VLDL particles. Here, we observed that Cideb is expressed in the jejunum and ileum sections of the small intestine, and its expression was induced by high-fat diet. Intragastric gavage with lipids resulted in the retention of lipids in the intestine in Cideb-deficient mice. In addition, we observed that mice with Cideb deficiency exhibited reduced intestinal chylomicron-TG secretion and increased lipid accumulation in the enterocytes. The sizes of chylomicrons secreted from the small intestine of Cideb-deficient mice were also smaller than those from wild-type mice. Furthermore, the overexpression of Cideb increased TG secretion and reduced lipid accumulation in the enterocyte-like Caco-2 cells. In addition, we proved that Cideb was localized to the ER and LDs and could interact with ApoB48 in Caco-2 cells. Overall, these data revealed that Cideb plays an important role in controlling intestinal chylomicron lipidation.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Zhang LJ,Wang C,Yuan Y,Wang H,Wu J,Liu F,Li L,Gao X,Zhao YL,Hu PZ,Li P,Ye Jdoi
10.1194/jlr.M046482subject
Has Abstractpub_date
2014-07-01 00:00:00pages
1279-87issue
7eissn
0022-2275issn
1539-7262pii
jlr.M046482journal_volume
55pub_type
杂志文章abstract::Recent reports from this laboratory indicate that exposure of cholesterol-loaded macrophages to high density lipoprotein 3 (HDL3) stimulates not only cholesterol efflux, but also results in a two- to threefold increase in apoE accumulation in the media (Dory, L., 1989. J. Lipid Res. 30: 809-816). The present experimen...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1991-05-01 00:00:00
abstract::Adipose differentiation-related protein (ADRP) is a lipid droplet-associated protein that is expressed in various tissues. In mice treated with the peroxisome proliferator-activated receptor alpha (PPARalpha) agonist Wy14,643 (Wy), hepatic mRNA and protein levels of ADRP as well as hepatic triglyceride content increas...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M500203-JLR200
更新日期:2006-02-01 00:00:00
abstract::(13)C-Cholesterol was produced with high efficiency by a genetically engineered yeast strain. The method produces ∼ 1 mg of cholesterol per gram of glucose using 100 ml of culture medium. Uniform 94% enrichment where the most abundant product is the fully enriched isotopomer (u-(13)C(27)) is obtained using (u-(13)C(6)...
journal_title:Journal of lipid research
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doi:10.1194/jlr.D014209
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M600135-JLR200
更新日期:2006-07-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1978-11-01 00:00:00
abstract::Inositol lipid kinases have been studied extensively in both plant and animal systems. However, major limitations for in vitro studies of recombinant lipid kinases are the low specific activity and instability of the purified proteins. Our goal was to determine if cyclodextrins would provide an effective substrate del...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.D400005-JLR200
更新日期:2004-09-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
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更新日期:1974-03-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00
abstract::Genetic defects in SAR1B GTPase inhibit chylomicron (CM) trafficking to the Golgi and result in a huge intraenterocyte lipid accumulation with a failure to release CMs and liposoluble vitamins into the blood circulation. The central aim of this study is to test the hypothesis that SAR1B deletion (SAR1B-/- ) disturbs e...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.RA119000119
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.RA119000256
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M600177-JLR200
更新日期:2006-09-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1989-06-01 00:00:00
abstract::Reduced estrogen levels result in loss of protection from coronary heart disease in postmenopausal women. Enhanced and diminished atherosclerosis have been associated with plasma levels of cholesteryl ester transfer protein (CETP); however, little is known about the role of CETP-ovarian hormone interactions in atherog...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.m100440-jlr200
更新日期:2003-01-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M700519-JLR200
更新日期:2008-04-01 00:00:00
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journal_title:Journal of lipid research
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doi:10.1194/jlr.RA120000704
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.D071068
更新日期:2016-11-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M400391-JLR200
更新日期:2005-03-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M064709
更新日期:2016-07-01 00:00:00
abstract::Lysophosphatidylcholine (lysoPC) is a bioactive phospholipid that is involved in atherogenesis and inflammatory processes. However, the present understanding of mechanisms whereby lysophosphatidylcholine exerts its pathophysiological actions is incomplete. In the present work, we show that lysoPC stimulates phospholip...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract::Studies were carried out to determine whether apolipoprotein (apo) A-II, like apoA-I, can recruit phospholipid and cholesterol from cell membranes, thereby forming nascent apoA-II-specific HDL. ApoA-II and apoA-I were purified from plasma and each was incubated with CHO cells at a concentration of 10 micrograms/ml. Li...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
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doi:10.1194/jlr.M300158-JLR200
更新日期:2004-01-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1972-01-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M600488-JLR200
更新日期:2007-04-01 00:00:00
abstract::Lec9 Chinese hamster ovary cells were found previously to be defective in the synthesis of N-linked glycans. This appeared to be the result of a defect in the synthesis of oligosaccharide lipid and lipid phosphate (Rosenwald, Stanley, and Krag. 1989. Mol. Cell. Biol. 9: 914-924). In this study we analyzed the steady s...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1990-03-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1977-07-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1998-05-01 00:00:00
abstract::In an effort tao simulate the effects of insulin on fat cells prepared from fresh adipose tissue, pieces of epididymal adipose tissue were cultured in a medium containing charcoal-treated bovine serum albumin (BSA) with a gas phase of 100% O2. Using this system, the insulin effect on [1-14C]glucose oxidation was retai...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1982-02-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M085274
更新日期:2018-08-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1989-09-01 00:00:00
abstract::Clostridium absonum was shown to metabolize primary bile acids to give rise to both 7-oxo bile acids and 7 beta-hydroxy (urso) bile acids. At relatively low redox potential (Eh) values, high yields of urso bile acids were achieved (60-75%). If, however, the Eh value of the culture was allowed to rise above approximate...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1982-07-01 00:00:00