Hepatitis B virus surface antigen and anti-hepatitis C virus rapid tests underestimate hepatitis prevalence among HIV-infected patients.

Abstract:

OBJECTIVES:In the case of coinfection with HIV and hepatitis B virus (HBV) and/or hepatitis C virus (HCV), hepatic disease progression is often accelerated, with higher rates of liver cirrhosis and liver-related mortality. We aimed to evaluate the performance of the rapid tests used routinely to detect HBV surface antigen (HBsAg) and anti-HCV among HIV-infected patients in Guinea-Bissau. METHODS:Blood samples from HIV-infected patients in Guinea-Bissau were stored after testing for HBsAg and anti-HCV with rapid tests. Samples were subsequently re-tested for HBsAg and anti-HCV in Denmark. RESULTS:Two rapid tests were used in Guinea-Bissau: HBsAg Strip Ref 2034 (VEDA.LAB, Alençon, France; sensitivity 62.3%; specificity 99.2%) and HEPA-SCAN (Bhat Bio-Tech, Bangalore, India; sensitivity 57.1%; specificity 99.7%). In the two tests the ability to obtain the correct outcome depended on the antigen and antibody concentrations, respectively. Sex, age, CD4 cell count and antiretroviral therapy status did not differ between false negative and true positive samples in either of the tests. The study is limited by a low number of anti-HCV positive samples. CONCLUSIONS:New diagnostic rapid tests should always be evaluated in the setting in which they will be used before implementation.

journal_name

HIV Med

journal_title

HIV medicine

authors

Hønge B,Jespersen S,Medina C,Té D,da Silva Z,Ostergaard L,Laursen A,Wejse C,Krarup H,Erikstrup C,Bissau HIV cohort study group.

doi

10.1111/hiv.12158

subject

Has Abstract

pub_date

2014-10-01 00:00:00

pages

571-6

issue

9

eissn

1464-2662

issn

1468-1293

journal_volume

15

pub_type

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