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Dissecting complex polyketide biosynthesis.

Abstract：

:Numerous bioactive natural products are synthesised by modular polyketide synthases. These compounds can be made in high yield by native multienzyme assembly lines. However, formation of analogues by genetically engineered systems is often considerably less efficient. Biochemical studies on intact polyketide synthase proteins have amassed a body of knowledge that is substantial but still incomplete. Recently, the constituent enzymes have been structurally characterised as discrete domains or didomains. These recombinant proteins have been used to reconstitute single extension cycles in vitro. This has given further insights into how the final stereochemistry of chiral centres in polyketides is determined. In addition, this approach has revealed how domains co-operate to ensure efficient transfer of growing intermediates along the assembly line. This work is leading towards more effective re-programming of these enzymes for use in synthesis of new medicinal compounds.

journal_name

Comput Struct Biotechnol J

journal_title

Computational and structural biotechnology journal

authors

Caffrey P

doi

10.5936/csbj.201210010

subject

Has Abstract

pub_date

2012-11-17 00:00:00

pages

e201210010

issn

2001-0370

pii

CSBJ-3-e201210010

journal_volume

3

pub_type

杂志文章,评审

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