Abstract:
:Acute lung injury (ALI) is characterized by pulmonary inflammation and edema. Innate immune cells (e.g., neutrophils and macrophages) are major contributors to inflammation in ALI. Less is known regarding the role of T cells. We examined the effects of rapamycin on inflammation in a LPS-induced murine model of ALI. Rapamycin was administered before and after initiation of injury. Inflammatory parameters, including bronchoalveolar lavage cell counts, T cell surface markers (i.e., cytotoxic T lymphocyte antigen 4 [CTLA4] and fork head-winged helix transcription factor [Foxp3]), T cell activation (CD69), IL-6, and IL-10 were analyzed. Rapamycin significantly decreased inflammatory parameters and decreased Foxp3, CTLA4, and CD69 in CD4(+) T cells. Rapamycin administration before or after the onset of lung injury, as well as systemically or by pulmonary routes, ameliorates inflammation in ALI.
journal_name
Am J Respir Cell Mol Biolauthors
Nakajima T,Lin KW,Li J,McGee HS,Kwan JM,Perkins DL,Finn PWdoi
10.1165/rcmb.2013-0171OCsubject
Has Abstractpub_date
2014-08-01 00:00:00pages
294-9issue
2eissn
1044-1549issn
1535-4989journal_volume
51pub_type
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