Abstract:
:Horizontal gene transfer (HGT) is a major force driving bacterial evolution. Because of their ability to cross inter-species barriers, bacterial plasmids are essential agents for HGT. This ability, however, poses specific requisites on plasmid physiology, in particular the need to overcome a multilevel selection process with opposing demands. We analyzed the transcriptional network of plasmid R388, one of the most promiscuous plasmids in Proteobacteria. Transcriptional analysis by fluorescence expression profiling and quantitative PCR revealed a regulatory network controlled by six transcriptional repressors. The regulatory network relied on strong promoters, which were tightly repressed in negative feedback loops. Computational simulations and theoretical analysis indicated that this architecture would show a transcriptional burst after plasmid conjugation, linking the magnitude of the feedback gain with the intensity of the transcriptional burst. Experimental analysis showed that transcriptional overshooting occurred when the plasmid invaded a new population of susceptible cells. We propose that transcriptional overshooting allows genome rebooting after horizontal gene transfer, and might have an adaptive role in overcoming the opposing demands of multilevel selection.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Fernandez-Lopez R,Del Campo I,Revilla C,Cuevas A,de la Cruz Fdoi
10.1371/journal.pgen.1004171subject
Has Abstractpub_date
2014-02-27 00:00:00pages
e1004171issue
2eissn
1553-7390issn
1553-7404pii
PGENETICS-D-13-01250journal_volume
10pub_type
杂志文章相关文献
PLoS Genetics文献大全abstract::The Hippo pathway regulates organ size, stem cell proliferation and tumorigenesis in adult organs. Whether the Hippo pathway influences establishment of stem cell niche size to accommodate changes in organ size, however, has received little attention. Here, we ask whether Hippo signaling influences the number of stem ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004962
更新日期:2015-02-02 00:00:00
abstract::The application of deep sequencing to map 5' capped transcripts has confirmed the existence of at least two distinct promoter classes in metazoans: "focused" promoters with transcription start sites (TSSs) that occur in a narrowly defined genomic span and "dispersed" promoters with TSSs that are spread over a larger w...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1001274
更新日期:2011-01-13 00:00:00
abstract::The High Pathogenicity Island of Yersinia pseudotuberculosis IP32637 was previously shown to be horizontally transferable as part of a large chromosomal segment. We demonstrate here that at low temperature other chromosomal loci, as well as a non-mobilizable plasmid (pUC4K), are also transferable. This transfer, desig...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002529
更新日期:2012-01-01 00:00:00
abstract::Microtubule-microfilament interactions are important for cytokinesis and subcellular localization of proteins and mRNAs. In the early zebrafish embryo, astral microtubule-microfilament interactions also facilitate a stereotypic segregation pattern of germ plasm ribonucleoparticles (GP RNPs), which is critical for thei...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003448
更新日期:2013-04-01 00:00:00
abstract::Systemic lupus erythematosus (SLE) is a genetically complex disease with heterogeneous clinical manifestations. A polymorphism in the STAT4 gene has recently been established as a risk factor for SLE, but the relationship with specific SLE subphenotypes has not been studied. We studied 137 SNPs in the STAT4 region gen...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000084
更新日期:2008-05-30 00:00:00
abstract::The Caenorhabditis elegans class A and B synthetic multivulva (synMuv) genes redundantly antagonize an EGF/Ras pathway to prevent ectopic vulval induction. We identify a class A synMuv mutation in the promoter of the lin-3 EGF gene, establishing that lin-3 is the key biological target of the class A synMuv genes in vu...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002418
更新日期:2011-12-01 00:00:00
abstract::Significant interest has emerged in mapping genetic susceptibility for complex traits through whole-genome association studies. These studies rely on the extent of association, i.e., linkage disequilibrium (LD), between single nucleotide polymorphisms (SNPs) across the human genome. LD describes the nonrandom associat...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.0020142
更新日期:2006-09-08 00:00:00
abstract::Formation of elaborately branched dendrites is necessary for the proper input and connectivity of many sensory neurons. Previous studies have revealed that dendritic growth relies heavily on ER-to-Golgi transport, Golgi outposts and endocytic recycling. How new membrane and associated cargo is delivered from the secre...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005484
更新日期:2015-09-22 00:00:00
abstract::In non-motile fungi, sexual reproduction relies on strong morphogenetic changes in response to pheromone signaling. We report here on a systematic screen for morphological abnormalities of the mating process in fission yeast Schizosaccharomyces pombe. We derived a homothallic (self-fertile) collection of viable deleti...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006721
更新日期:2017-04-14 00:00:00
abstract::The phenotypic heterogeneity that characterizes human cancers reflects the enormous genetic complexity of the oncogenic process. This complexity can also be seen in mouse models where it is frequently observed that in addition to the initiating genetic alteration, the resulting tumor harbors additional, somatically ac...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000640
更新日期:2009-09-01 00:00:00
abstract::DNA methylation is an ancient molecular modification found in most eukaryotes. In plants, DNA methylation is not only critical for transcriptionally silencing transposons, but can also affect phenotype by altering expression of protein coding genes. The extent of its contribution to phenotypic diversity over evolution...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004785
更新日期:2014-11-13 00:00:00
abstract::The contribution of MEF2 TFs to the tumorigenic process is still mysterious. Here we clarify that MEF2 can support both pro-oncogenic or tumor suppressive activities depending on the interaction with co-activators or co-repressors partners. Through these interactions MEF2 supervise histone modifications associated wit...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006752
更新日期:2017-04-18 00:00:00
abstract::Zygotic gene expression programs control cell differentiation in vertebrate development. In Xenopus, these programs are initiated by local induction of regulatory genes through maternal signaling activities in the wake of zygotic genome activation (ZGA) at the midblastula transition (MBT). These programs lay down the ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006757
更新日期:2017-05-12 00:00:00
abstract::Centromere sequences are not conserved between species, and there is compelling evidence for epigenetic regulation of centromere identity, with location being dictated by the presence of chromatin containing the histone H3 variant CENP-A. Paradoxically, in most organisms CENP-A chromatin generally occurs on particular...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004986
更新日期:2015-03-04 00:00:00
abstract::Plant microRNAs (miRNAs) are critical regulators of gene expression, however little attention has been given to the principles governing miRNA silencing efficacy. Here, we utilize the highly conserved Arabidopsis miR159-MYB33/MYB65 regulatory module to explore these principles. Firstly, we show that perfect central co...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004232
更新日期:2014-03-13 00:00:00
abstract::Chemosensory pheromonal information regulates aggression and reproduction in many species, but how pheromonal signals are transduced to reliably produce behavior is not well understood. Here we demonstrate that the pheromonal signals detected by Gr32a-expressing chemosensory neurons to enhance male aggression are filt...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004356
更新日期:2014-05-22 00:00:00
abstract::Translational systems can respond promptly to sudden environmental changes to provide rapid adaptations to environmental stress. Unlike the well-studied translational responses to oxidative stress in eukaryotic systems, little is known regarding how prokaryotes respond rapidly to oxidative stress in terms of translati...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005302
更新日期:2015-06-19 00:00:00
abstract::Rhizobial infection of legume root hairs requires a rearrangement of the actin cytoskeleton to enable the establishment of plant-made infection structures called infection threads. In the SCAR/WAVE (Suppressor of cAMP receptor defect/WASP family verpolin homologous protein) actin regulatory complex, the conserved N-te...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005623
更新日期:2015-10-30 00:00:00
abstract::The epigenetic regulation of gene expression by the covalent modification of histones is a fundamental mechanism required for the proper differentiation of germ line cells during development. Trimethylation of histone 3 lysine 9 (H3K9me3) leads to chromatin silencing and the formation of heterochromatin by recruitment...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000644
更新日期:2009-09-01 00:00:00
abstract::In C. elegans, removal of the germline extends lifespan significantly. We demonstrate that the nuclear hormone receptor, NHR-49, enables the response to this physiological change by increasing the expression of genes involved in mitochondrial β-oxidation and fatty-acid desaturation. The coordinated augmentation of the...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004829
更新日期:2014-12-04 00:00:00
abstract::The genetic impact associated to the Neolithic spread in Europe has been widely debated over the last 20 years. Within this context, ancient DNA studies have provided a more reliable picture by directly analyzing the protagonist populations at different regions in Europe. However, the lack of available data from the o...
journal_title:PLoS genetics
pub_type: 历史文章,杂志文章
doi:10.1371/journal.pgen.1004401
更新日期:2014-06-05 00:00:00
abstract::Fibroblasts are ubiquitous mesenchymal cells with many vital functions during development, tissue repair, and disease. Fibroblasts from different anatomic sites have distinct and characteristic gene expression patterns, but the principles that govern their molecular specialization are poorly understood. Spatial organi...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.0020119
更新日期:2006-07-01 00:00:00
abstract::Sideroblastic anemias are acquired or inherited anemias that result in a decreased ability to synthesize hemoglobin in red blood cells and result in the presence of iron deposits in the mitochondria of red blood cell precursors. A common subtype of congenital sideroblastic anemia is due to autosomal recessive mutation...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005783
更新日期:2016-01-28 00:00:00
abstract::A large fraction of human genes are regulated by genetic variation near the transcribed sequence (cis-eQTL, expression quantitative trait locus), and many cis-eQTLs have implications for human disease. Less is known regarding the effects of genetic variation on expression of distant genes (trans-eQTLs) and their biolo...
journal_title:PLoS genetics
pub_type: 杂志文章,随机对照试验
doi:10.1371/journal.pgen.1004818
更新日期:2014-12-04 00:00:00
abstract::Tripartite Motif (TRIM) ubiquitin ligases act in the innate immune response against viruses. One of the best characterized members of this family, TRIM5α, serves as a potent retroviral restriction factor with activity against HIV. Here, we characterize what are likely to be the youngest TRIM genes in the human genome....
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002388
更新日期:2011-12-01 00:00:00
abstract::Pregnancy-induced noncoding RNA (PINC) and retinoblastoma-associated protein 46 (RbAp46) are upregulated in alveolar cells of the mammary gland during pregnancy and persist in alveolar cells that remain in the regressed lobules following involution. The cells that survive involution are thought to function as alveolar...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002840
更新日期:2012-01-01 00:00:00
abstract::Metabolism, the conversion of nutrients into usable energy and biochemical building blocks, is an essential feature of all cells. The genetic factors responsible for inter-individual metabolic variability remain poorly understood. To investigate genetic causes of metabolome variation, we measured the concentrations of...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004142
更新日期:2014-03-06 00:00:00
abstract::Arsenic is a well-established human carcinogen of poorly understood mechanism of genotoxicity. It is generally accepted that arsenic acts indirectly by generating oxidative DNA damage that can be converted to replication-dependent DNA double-strand breaks (DSBs), as well as by interfering with DNA repair pathways and ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003640
更新日期:2013-01-01 00:00:00
abstract::Promoters are structurally and functionally diverse gene regulatory regions. The presence or absence of sequence motifs and the spacing between the motifs defines the properties of promoters. Recent alternative promoter usage analyses in Drosophila melanogaster revealed that transposable elements significantly contrib...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006249
更新日期:2016-08-12 00:00:00
abstract::While the molecular mechanisms of glucocorticoid regulation of transcription have been studied in detail, the global networks regulated by the glucocorticoid receptor (GR) remain unknown. To address this question, we performed an orthogonal analysis to identify direct targets of the GR. First, we analyzed the expressi...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.0010016
更新日期:2005-08-01 00:00:00