Abstract:
:Zygotic gene expression programs control cell differentiation in vertebrate development. In Xenopus, these programs are initiated by local induction of regulatory genes through maternal signaling activities in the wake of zygotic genome activation (ZGA) at the midblastula transition (MBT). These programs lay down the vertebrate body plan through gastrulation and neurulation, and are accompanied by massive changes in chromatin structure, which increasingly constrain cellular plasticity. Here we report on developmental functions for Brahma related gene 1 (Brg1), a key component of embyronic SWI/SNF chromatin remodeling complexes. Carefully controlled, global Brg1 protein depletion in X. tropicalis and X. laevis causes embryonic lethality or developmental arrest from gastrulation on. Transcriptome analysis at late blastula, before development becomes arrested, indicates predominantly a role for Brg1 in transcriptional activation of a limited set of genes involved in pattern specification processes and nervous system development. Mosaic analysis by targeted microinjection defines Brg1 as an essential amplifier of gene expression in dorsal (BCNE/Nieuwkoop Center) and ventral (BMP/Vent) signaling centers. Moreover, Brg1 is required and sufficient for initiating axial patterning in cooperation with maternal Wnt signaling. In search for a common denominator of Brg1 impact on development, we have quantitatively filtered global mRNA fluctuations at MBT. The results indicate that Brg1 is predominantly required for genes with the highest burst of transcriptional activity. Since this group contains many key developmental regulators, we propose Brg1 to be responsible for raising their expression above threshold levels in preparation for embryonic patterning.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Wagner G,Singhal N,Nicetto D,Straub T,Kremmer E,Rupp RAWdoi
10.1371/journal.pgen.1006757subject
Has Abstractpub_date
2017-05-12 00:00:00pages
e1006757issue
5eissn
1553-7390issn
1553-7404pii
PGENETICS-D-16-02269journal_volume
13pub_type
杂志文章相关文献
PLoS Genetics文献大全abstract::With multiple genome-wide association studies (GWAS) performed across autoimmune diseases, there is a great opportunity to study the homogeneity of genetic architectures across autoimmune disease. Previous approaches have been limited in the scope of their analysis and have failed to properly incorporate the direction...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000792
更新日期:2009-12-01 00:00:00
abstract::Variably expressive copy-number variants (CNVs) are characterized by extensive phenotypic heterogeneity of neuropsychiatric phenotypes. Approaches to identify single causative genes for these phenotypes within each CNV have not been successful. Here, we posit using multiple lines of evidence, including pathogenicity m...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007879
更新日期:2019-01-17 00:00:00
abstract::The t-haplotype, a variant form of the t-complex region on mouse chromosome 17, acts as selfish genetic element and is transmitted at high frequencies (> 95%) from heterozygous (t/+) males to their offspring. This phenotype is termed transmission ratio distortion (TRD) and is caused by the interaction of the t-complex...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002567
更新日期:2012-01-01 00:00:00
abstract::Stem cells in tissues reside in and receive signals from local microenvironments called niches. Understanding how multiple signals within niches integrate to control stem cell function is challenging. The Drosophila testis stem cell niche consists of somatic hub cells that maintain both germline stem cells and somatic...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004713
更新日期:2014-11-06 00:00:00
abstract::Genome-wide association studies (GWAS) have identified chromosomal loci that affect risk of coronary heart disease (CHD) independent of classical risk factors. One such association signal has been identified at 6q23.2 in both Caucasians and East Asians. The lead CHD-associated polymorphism in this region, rs12190287, ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004263
更新日期:2014-03-27 00:00:00
abstract::We carried out an admixture analysis of a sample comprising 1,019 individuals from all the provinces of Cuba. We used a panel of 128 autosomal Ancestry Informative Markers (AIMs) to estimate the admixture proportions. We also characterized a number of haplogroup diagnostic markers in the mtDNA and Y-chromosome in orde...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004488
更新日期:2014-07-24 00:00:00
abstract::A variety of models have been proposed to explain regions of recurrent somatic copy number alteration (SCNA) in human cancer. Our study employs Whole Genome DNA Sequence (WGS) data from tumor samples (n = 103) to comprehensively assess the role of the Knudson two hit genetic model in SCNA generation in prostate cancer...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007001
更新日期:2017-09-25 00:00:00
abstract::Male fertility requires the continuous production of high quality motile spermatozoa in abundance. Alterations in all three metrics cause oligoasthenoteratozoospermia, the leading cause of human sub/infertility. Post-mitotic spermatogenesis inclusive of several meiotic stages and spermiogenesis (terminal spermatozoa d...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004597
更新日期:2014-10-16 00:00:00
abstract::The circadian clock coordinates physiology and metabolism. mTOR (mammalian/mechanistic target of rapamycin) is a major intracellular sensor that integrates nutrient and energy status to regulate protein synthesis, metabolism, and cell growth. Previous studies have identified a key role for mTOR in regulating photic en...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007369
更新日期:2018-05-11 00:00:00
abstract::Polymyxin is the last line of defense against severe infections caused by carbapenem-resistant gram-negative pathogens. The emergence of transferable MCR-1/2 polymyxin resistance greatly challenges the renewed interest in colistin (polymyxin E) for clinical treatments. Recent studies have suggested that Moraxella spec...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007389
更新日期:2018-05-14 00:00:00
abstract::Prolific sheep have proven to be a valuable model to identify genes and mutations implicated in female fertility. In the Lacaune sheep breed, large variation in litter size is genetically determined by the segregation of a fecundity major gene influencing ovulation rate, named FecL and its prolific allele FecL(L) . Ou...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003809
更新日期:2013-01-01 00:00:00
abstract::During neural circuit formation, most axons are guided to complex environments, coming into contact with multiple potential synaptic partners. However, it is critical that they recognize specific neurons with which to form synapses. Here, we utilize the split GFP-based marker Neuroligin-1 GFP Reconstitution Across Syn...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007312
更新日期:2018-05-09 00:00:00
abstract::House dust mites are common pests with an unusual evolutionary history, being descendants of a parasitic ancestor. Transition to parasitism is frequently accompanied by genome rearrangements, possibly to accommodate the genetic change needed to access new ecology. Transposable element (TE) activity is a source of geno...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007183
更新日期:2018-01-29 00:00:00
abstract::During neurogenesis, transcription factors combinatorially specify neuronal fates and then differentiate subtype identities by inducing subtype-specific gene expression profiles. But how is neuronal subtype identity maintained in mature neurons? Modeling this question in two Drosophila neuronal subtypes (Tv1 and Tv4),...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002501
更新日期:2012-01-01 00:00:00
abstract::Adipose tissue lipolysis occurs during the development of heart failure as a consequence of chronic adrenergic stimulation. However, the impact of enhanced adipose triacylglycerol hydrolysis mediated by adipose triglyceride lipase (ATGL) on cardiac function is unclear. To investigate the role of adipose tissue lipolys...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007171
更新日期:2018-01-10 00:00:00
abstract::The mechanism of circadian oscillations in mammals is cell autonomous and is generated by a set of genes that form a transcriptional autoregulatory feedback loop. While these "clock genes" are well conserved among animals, their specific functions remain to be fully understood and their roles in central versus periphe...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.0030033
更新日期:2007-02-23 00:00:00
abstract::Two-component signaling systems (TCS) regulate bacterial responses to environmental signals through the process of protein phosphorylation. Specifically, sensor histidine kinases (SK) recognize signals and propagate the response via phosphorylation of a cognate response regulator (RR) that functions to initiate transc...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007714
更新日期:2018-10-22 00:00:00
abstract::From Darwin's study of the Galapagos and Wallace's study of Indonesia, islands have played an important role in evolutionary investigations, and radiations within archipelagos are readily interpreted as supporting the conventional view of allopatric speciation. Even during the ongoing paradigm shift towards other mode...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000929
更新日期:2010-04-29 00:00:00
abstract::[This corrects the article DOI: 10.1371/journal.pgen.1000832.]. ...
journal_title:PLoS genetics
pub_type: 杂志文章,已发布勘误
doi:10.1371/journal.pgen.1007392
更新日期:2018-05-16 00:00:00
abstract::Orienting the causal relationship between pairs of traits is a fundamental task in scientific research with significant implications in practice, such as in prioritizing molecular targets and modifiable risk factors for developing therapeutic and interventional strategies for complex diseases. A recent method, called ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1009105
更新日期:2020-11-02 00:00:00
abstract::Race, specifically African ancestry, and obesity are important risk factors for uterine fibroids, and likely interact to provide the right conditions for fibroid growth. However, existing studies largely focus on the main-effects rather than their interaction. Here, we firstly provide evidence for interaction between ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006871
更新日期:2017-07-17 00:00:00
abstract::To understand the genetic mechanisms leading to phenotypic differentiation, it is important to identify genomic regions under selection. We scanned the genome of two chicken lines from a single trait selection experiment, where 50 generations of selection have resulted in a 9-fold difference in body weight. Analyses o...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1001188
更新日期:2010-11-04 00:00:00
abstract::Systems biology approaches that are based on the genetics of gene expression have been fruitful in identifying genetic regulatory loci related to complex traits. We use microarray and genetic marker data from an F2 mouse intercross to examine the large-scale organization of the gene co-expression network in liver, and...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.0020130
更新日期:2006-08-18 00:00:00
abstract::Autosomal dominant polycystic kidney disease (ADPKD) is characterized by formation of renal cysts that destroy the kidney. Mutations in PKD1 and PKD2, encoding polycystins-1 and -2, cause ADPKD. Polycystins are thought to function in primary cilia, but it is not well understood how these and other proteins are targete...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1001361
更新日期:2011-04-01 00:00:00
abstract::Neisseria meningitidis is the primary causative agent of bacterial meningitis. The genome is rich in repetitive DNA and almost 2% is occupied by a diminutive transposon called the Correia element. Here we report a bioinformatic analysis defining eight subtypes of the element with four distinct types of ends. Transcrip...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1001277
更新日期:2011-01-20 00:00:00
abstract::Release factors (RFs) govern the termination phase of protein synthesis. Human mitochondria harbor four different members of the class 1 RF family: RF1Lmt/mtRF1a, RF1mt, C12orf65 and ICT1. The homolog of the essential ICT1 factor is widely distributed in bacteria and organelles and has the peculiar feature in human mi...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004616
更新日期:2014-09-18 00:00:00
abstract::Traditional Chinese medicine (TCM) has been practiced for thousands of years, but only within the last few decades has its use become more widespread outside of Asia. Concerns continue to be raised about the efficacy, legality, and safety of many popular complementary alternative medicines, including TCMs. Ingredients...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002657
更新日期:2012-01-01 00:00:00
abstract::Breast cancer is the most common cancer in women in developed countries, and the contribution of genetic susceptibility to breast cancer development has been well-recognized. However, a great proportion of these hereditary predisposing factors still remain unidentified. To examine the contribution of rare copy number ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002734
更新日期:2012-01-01 00:00:00
abstract::Neurofibromatosis type 1 is a monogenetic disorder that predisposes individuals to tumor formation and cognitive and behavioral symptoms. The neuronal circuitry and developmental events underlying these neurological symptoms are unknown. To better understand how mutations of the underlying gene (NF1) drive behavioral ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008920
更新日期:2020-07-22 00:00:00
abstract::Plant stress responses require both protective measures that reduce or restore stress-inflicted damage to cellular structures and mechanisms that efficiently remove damaged and toxic macromolecules, such as misfolded and damaged proteins. We have recently reported that NBR1, the first identified plant autophagy adapto...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004116
更新日期:2014-01-30 00:00:00