Mutagenesis by the autoxidation of iron with isolated DNA.

abstract：:Recent advances in metabolic engineering have demonstrated that microbial biosynthesis can provide a viable alternative to chemical synthesis for the production of bulk and fine chemicals. Introduction of a new biosynthetic pathway typically requires the expression of multiple heterologous enzymes in the production ho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Michener JK,Nielsen J,Smolke CD

更新日期：2012-11-20 00:00:00

abstract：:Allostery plays a key role in the regulation of the activity and function of many biomolecules. And although many ligands act through allostery, no systematic use is made of it in drug design strategies. Here we describe a procedure for identifying the regions of a protein that can be used to control its activity thro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Laine E,Goncalves C,Karst JC,Lesnard A,Rault S,Tang WJ,Malliavin TE,Ladant D,Blondel A

更新日期：2010-06-22 00:00:00

abstract：:The oncoproteins MDM2 and MDMX negatively regulate the activity and stability of the tumor suppressor protein p53--a cellular process initiated by MDM2 and/or MDMX binding to the N-terminal transactivation domain of p53. MDM2 and MDMX in many tumors confer p53 inactivation and tumor survival, and are important molecul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pazgier M,Liu M,Zou G,Yuan W,Li C,Li C,Li J,Monbo J,Zella D,Tarasov SG,Lu W

更新日期：2009-03-24 00:00:00

abstract：:Whether the regulatory protein of an inducible operon for a catabolic pathway is a positive or a negative element in the control of expression is correlated with the frequent or infrequent presence, respectively, of the system's substrate in the natural environment. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Savageau MA

更新日期：1974-06-01 00:00:00

abstract：:A 92-amino acid polypeptide, alpha-inhibin-92 (alpha-IB-92), has been isolated and characterized from human seminal plasma and found to be active in suppressing follicle-stimulating hormone (FSH) release in vitro. In the present in vivo study, intravenous injection of synthetic alpha-IB-92 (4 and 20 micrograms) signif...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu WH,McCann SM,Li CH

更新日期：1988-01-01 00:00:00

abstract：:We show that the diverse ecoregions of Madagascar share one distinctive climatic feature: unpredictable intra- or interannual precipitation compared with other regions with comparable rainfall. Climatic unpredictability is associated with unpredictable patterns of fruiting and flowering. It is argued that these featur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dewar RE,Richard AF

更新日期：2007-08-21 00:00:00

abstract：:We have developed a modified rhodamine (Rho) staining procedure to study uptake and efflux in murine hematopoietic stem cells. Distinct populations of Rho++ (bright), Rho+ (dull), and Rho- (negative) cells could be discriminated. Sorted Rho- cells were subjected to a second Rho staining procedure with the P-glycoprote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zijlmans JM,Visser JW,Kleiverda K,Kluin PM,Willemze R,Fibbe WE

更新日期：1995-09-12 00:00:00

abstract：:The Raf-1 serine/threonine kinase is a key protein involved in the transmission of many growth and developmental signals. In this report, we show that autoinhibition mediated by the noncatalytic, N-terminal regulatory region of Raf-1 is an important mechanism regulating Raf-1 function. The inhibition of the regulatory...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cutler RE Jr,Stephens RM,Saracino MR,Morrison DK

更新日期：1998-08-04 00:00:00

abstract：:Cyclonic atmospheric vortices of varying intensity, collectively known as low-pressure systems (LPS), travel northwest across central India and produce more than half of the precipitation received by that fertile region and its ∼600 million inhabitants. Yet, future changes in LPS activity are poorly understood, due in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sandeep S,Ajayamohan RS,Boos WR,Sabin TP,Praveen V

更新日期：2018-03-13 00:00:00

abstract：:After prolonged visual adaptation of Drosophila, dramatic long-term changes of in vitro phosphorylation of a 50-kDa brain protein that is identical to the Ca2+/calmodulin-dependent protein kinase (EC 2.7.1.37) can be measured in isolated heads. By selective receptor cell desensitization in blue light, subcellular dist...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Willmund R,Mitschulat H,Schneider K

更新日期：1986-12-01 00:00:00

abstract：:RNA-protein interactions underlie a wide range of cellular processes. Improved methods are needed to systematically map RNA-protein interactions in living cells in an unbiased manner. We used two approaches to target the engineered peroxidase APEX2 to specific cellular RNAs for RNA-centered proximity biotinylation of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han S,Zhao BS,Myers SA,Carr SA,He C,Ting AY

更新日期：2020-09-08 00:00:00

abstract：:Significantly above-chance detection of stimuli presented within the field defect of patients with postgeniculate lesions is termed "blindsight." It has been proposed that those with blindsight are more likely to benefit from visual rehabilitation by repeated stimulation, leading to increased visual sensitivity within...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sahraie A,Trevethan CT,MacLeod MJ,Urquhart J,Weiskrantz L

更新日期：2013-11-05 00:00:00

abstract：:Cedar virus (CedV) is a bat-borne henipavirus related to Nipah virus (NiV) and Hendra virus (HeV), zoonotic agents of fatal human disease. CedV receptor-binding protein (G) shares only ∼30% sequence identity with those of NiV and HeV, although they can all use ephrin-B2 as an entry receptor. We demonstrate that CedV a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Laing ED,Navaratnarajah CK,Cheliout Da Silva S,Petzing SR,Xu Y,Sterling SL,Marsh GA,Wang LF,Amaya M,Nikolov DB,Cattaneo R,Broder CC,Xu K

更新日期：2019-10-08 00:00:00

abstract：:The type 6 secretion system (T6SS) is a nanomachine used by many Gram-negative bacteria, including Vibrio cholerae, to deliver toxic effector proteins into adjacent eukaryotic and bacterial cells. Because the activity of the T6SS is dependent on direct contact between cells, its activity is limited to bacteria growing...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Toska J,Ho BT,Mekalanos JJ

更新日期：2018-07-31 00:00:00

abstract：:Branching morphogenesis in the mammary gland is achieved by the migration of epithelial cells through a microenvironment consisting of stromal cells and extracellular matrix (ECM). Here we show that galectin-1 (Gal-1), an endogenous lectin that recognizes glycans bearing N-acetyllactosamine (LacNAc) epitopes, induces ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bhat R,Belardi B,Mori H,Kuo P,Tam A,Hines WC,Le QT,Bertozzi CR,Bissell MJ

更新日期：2016-08-16 00:00:00

abstract：:CHD7 encodes an ATP-dependent chromatin remodeling factor. Mutation of this gene causes multiple developmental disorders, including CHARGE (Coloboma of the eye, Heart defects, Atresia of the choanae, Retardation of growth/development, Genital abnormalities, and Ear anomalies) syndrome, in which conotruncal anomalies a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yan S,Thienthanasit R,Chen D,Engelen E,Brühl J,Crossman DK,Kesterson R,Wang Q,Bouazoune K,Jiao K

更新日期：2020-11-17 00:00:00

abstract：:Double-stranded oligodeoxynucleotides that represent protein binding sites in the regulatory regions of the mouse genes encoding transthyretin (TTR) and alpha 1-antitrypsin (alpha 1-AT) bound a nuclear protein factor(s) found mainly in hepatocytes. A site in the regulatory region of the gene encoding rat serum albumin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Costa RH,Grayson DR,Xanthopoulos KG,Darnell JE Jr

更新日期：1988-06-01 00:00:00

abstract：:Research on global patterns of diversity has been dominated by studies seeking explanations for the equator-to-poles decline in richness of most groups of organisms, namely the latitudinal diversity gradient. A problem with this gradient is that it conflates two key explanations, namely biome stability (age and area) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Colville JF,Beale CM,Forest F,Altwegg R,Huntley B,Cowling RM

更新日期：2020-08-18 00:00:00

abstract：:Recent theory suggests that animals should switch facultatively among canonical movement modes as a complex function of internal state, landscape characteristics, motion capacity, and navigational capacity. We tested the generality of this paradigm for free-ranging elk (Cervus elaphus) over 5 orders of magnitude in ti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fryxell JM,Hazell M,Börger L,Dalziel BD,Haydon DT,Morales JM,McIntosh T,Rosatte RC

更新日期：2008-12-09 00:00:00

abstract：:The neurotrophin (NT) brain-derived neurotrophic factor (BDNF) plays an essential role in the formation of long-term potentiation (LTP). Here, we address whether this modulation by BDNF requires its continuous presence, or whether a local increase in BDNF is necessary during a specific time period of LTP initiation. U...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gärtner A,Staiger V

更新日期：2002-04-30 00:00:00

abstract：:Optimization theory has been used to analyze evolutionary adaptation. This theory has explained many features of biological systems, from the genetic code to animal behavior. However, these systems show important deviations from optimality. Typically, these deviations are large in some particular components of the sys...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pérez-Escudero A,Rivera-Alba M,de Polavieja GG

更新日期：2009-12-01 00:00:00

abstract：:Campylobacter jejuni is the leading cause of acute bacterial diarrhea worldwide and is implicated in development of Guillain-Barré syndrome. Two major surface features, the outer membrane lipooligosaccharide and flagella, are highly variable and are often targets for modification. Presumably, these modifications provi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cullen TW,Trent MS

更新日期：2010-03-16 00:00:00

abstract：:The bryostatins are macrocyclic lactones, extracted from the marine bryozoan Bugula neritina, and have been reported to be potent antineoplastic agents. Results described here demonstrate that the bryostatins may also be useful as stimulators of normal human hematopoietic cells since they can (i) directly stimulate bo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： May WS,Sharkis SJ,Esa AH,Gebbia V,Kraft AS,Pettit GR,Sensenbrenner LL

更新日期：1987-12-01 00:00:00

abstract：:microRNAs comprise a few percent of animal genes and have been recognized as important regulators of a diverse range of biological processes. Understanding the biological functions of miRNAs requires effective means to identify their targets. Combined efforts from computational prediction, miRNA over-expression or dep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hong X,Hammell M,Ambros V,Cohen SM

更新日期：2009-09-01 00:00:00

abstract：:Controls of stem cell maintenance and early differentiation are known in several systems. However, the progression from stem cell self-renewal to overt signs of early differentiation is a poorly understood but important problem in stem cell biology. The Caenorhabditis elegans germ line provides a genetically defined m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cinquin O,Crittenden SL,Morgan DE,Kimble J

更新日期：2010-02-02 00:00:00

abstract：:A simple model is described for calculating the electrostatic energy of lipid domains at the air-water interface, taking account of dipole-dipole repulsions between the lipid molecules themselves, as well as interactions between the molecular dipoles and image dipoles in the subphase. The model assumes that the molecu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McConnell HM,Bazaliy YB

更新日期：1995-09-12 00:00:00

abstract：:A sensitive assay using biotinylated ubiquitin revealed extensive ubiquitination of the large subunit of RNA polymerase II during incubations of transcription reactions in vitro. Phosphorylation of the repetitive carboxyl-terminal domain of the large subunit was a signal for ubiquitination. Specific inhibitors of cycl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitsui A,Sharp PA

更新日期：1999-05-25 00:00:00

abstract：:V79 Chinese hamster fibroblasts are widely used for mutagenicity testing but have the serious limitation that they do not express cytochromes P-450, which are needed for the activation of many promutagens to mutagenic metabolites. A full-length cDNA clone encoding the monooxygenase cytochrome P-450IIB1 under control o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doehmer J,Dogra S,Friedberg T,Monier S,Adesnik M,Glatt H,Oesch F

更新日期：1988-08-01 00:00:00

abstract：:To study the structure and function of the gene for parathyroid hormone, we obtained recombinant plasmids containing bovine parathyroid hormone cDNA. The nucleotide sequence at the 5' terminus (relative to the sense strand) of the cDNA insert in a recombinant plasmid, pPTHi4, was different from that previously reporte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weaver CA,Gordon DF,Kemper B

更新日期：1981-07-01 00:00:00

abstract：:Saccharopolyspora erythraea makes erythromycin, an antibiotic commonly used in human medicine. Unusually, the erythromycin biosynthetic (ery) cluster lacks a pathway-specific regulatory gene. We isolated a transcriptional regulator of the ery biosynthetic genes from S. erythraea and found that this protein appears to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chng C,Lum AM,Vroom JA,Kao CM

更新日期：2008-08-12 00:00:00