Abstract：
:A plethora of biological and biotechnological processes involve the enzymatic remodelling of carbohydrates in complex mixtures whose compositions affect both the processes and products. In the current study, we employed high-resolution (1)H NMR spectroscopy for the analysis of cereal-derived carbohydrate mixtures as exemplified on six beer samples of different styles. Structural assignments of more than 50 carbohydrate moieties were obtained using (1)H1-(1)H2 groups as structural reporters. Spectroscopically resolved carbohydrates include more than ''20 different'' small carbohydrates with more than 38 isomeric forms in addition to cereal polysaccharide fragments with suspected organoleptic and prebiotic function. Structural motifs at the cleavage sites of starch, β-glucan and arabinoxylan fragments were identified, showing different extent and specificity of enzymatic polysaccharide cleavage during the production of different beer samples. Diffusion ordered spectroscopy supplied independent size information for the characterisation and identification of polysaccharide fragments, indicating the presence especially of high molecular weight arabinoxylan fragments in the final beer.
journal_name
Food Chemjournal_title
Food chemistryauthors
Petersen BO,Nilsson M,Bøjstrup M,Hindsgaul O,Meier Sdoi
10.1016/j.foodchem.2013.10.136subject
Has Abstractpub_date
2014-05-01 00:00:00pages
65-72eissn
0308-8146issn
1873-7072pii
S0308-8146(13)01585-9journal_volume
150pub_type
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