Inter-α-inhibitor blocks epithelial sodium channel activation and decreases nasal potential differences in ΔF508 mice.

abstract：:The reasons for bacterial proliferation in the lungs of mechanically ventilated patients are poorly understood. We hypothesized that prolonged cyclic stretch of lung cells influenced bacterial growth. Human alveolar type II-like A549 cells were submitted in vitro to prolonged cyclic stretch. Bacteria were cultured in ...

journal_title：American journal of respiratory cell and molecular biology

authors： Pugin J,Dunn-Siegrist I,Dufour J,Tissières P,Charles PE,Comte R

更新日期：2008-03-01 00:00:00

abstract：:Adenosine triphosphate (ATP) acting through epithelial nucleotide receptors exerts multiple physiologic actions on airway mucociliary clearance and caliber. However, the effect of ATP on arachidonate metabolism in the airway remains unknown. In this study, the ability of ATP to regulate eicosanoid production was studi...

journal_title：American journal of respiratory cell and molecular biology

authors： Aksoy MO,Borenstein M,Li XX,Kelsen SG

更新日期：1995-10-01 00:00:00

abstract：:To evaluate the response of an epithelial barrier to a moderate but sustained oxidative stress, we cultured monolayers of Madin Darby canine kidney cells on microporous filters and exposed them to the hypoxanthine-xanthine oxidase (HX-XO) reaction. The transepithelial permeability coefficient for mannitol (Pm) was ass...

journal_title：American journal of respiratory cell and molecular biology

authors： Rochat T,Burkhard C,Finci-Cerkez V,Meda P

更新日期：1993-11-01 00:00:00

abstract：:Patients with systemic hypertension of various etiologies maintain their pulmonary artery pressures within normal limits. We have reported in isolated perfused rat lungs that low basal tone appears to be regulated by nitric oxide (NO)-dependent and -independent mechanisms of soluble guanylate cyclase activation, and s...

journal_title：American journal of respiratory cell and molecular biology

authors： Itani MM,Talerico MG,Pino PA,Burke-Wolin T

更新日期：1996-10-01 00:00:00

abstract：:We examined the mechanisms underlying tachyphylaxis to beta-adrenergic receptor agonists (beta-agonists) in tracheal smooth muscle. Simultaneous measurements of isometric tension and intracellular Ca2+ concentration ([Ca2+]i) using fura-2-loaded guinea pig tracheas showed that the inhibitory effects of isoproterenol (...

journal_title：American journal of respiratory cell and molecular biology

authors： Kume H,Ishikawa T,Oguma T,Ito S,Shimokata K,Kotlikoff MI

更新日期：2003-09-01 00:00:00

abstract：:It is generally important to elucidate airway epithelial cell lineages and to identify multipotent progenitors as targets for gene therapy. Stem (S) cells are typically present in specialized compartments spatially proximal to their differentiated progeny, but an equivalent paradigm has not been demonstrated in the ai...

journal_title：American journal of respiratory cell and molecular biology

authors： Borthwick DW,Shahbazian M,Krantz QT,Dorin JR,Randell SH

更新日期：2001-06-01 00:00:00

abstract：:We examined the potential of several epithelial-derived factors to enhance neutrophil activation and survival. Neutrophils incubated in the presence of supernatants from nasal-derived primary epithelial cultures had significantly increased survival compared with neutrophils cultured in media alone. Of the cytokines re...

journal_title：American journal of respiratory cell and molecular biology

authors： Daffern PJ,Jagels MA,Hugli TE

更新日期：1999-08-01 00:00:00

abstract：:The surfactant-associated proteins SP-A and SP-D are members of a family of collagenous host defense lectins, designated collectins. There is increasing evidence that these pulmonary epithelial-derived proteins are important components of the innate immune response to microbial challenge, and that they participate in ...

journal_title：American journal of respiratory cell and molecular biology

authors： Crouch EC

更新日期：1998-08-01 00:00:00

abstract：:The experiments described herein define a unique program of polarization of suspended human eosinophils stimulated with IL-5 family cytokines. We found that eosinophil granules and the nucleus move in opposite directions to form, respectively, a granular compartment and the nucleopod, a specialized uropod occupied by ...

journal_title：American journal of respiratory cell and molecular biology

authors： Han ST,Mosher DF

更新日期：2014-03-01 00:00:00

abstract：:New approaches for improving tuberculosis (TB) control using adjunct host-directed cellular and repurposed drug therapies are needed. Autophagy plays a crucial role in the response to TB, and a variety of autophagy-inducing drugs that are currently available for various medical conditions may serve as an adjunct treat...

journal_title：American journal of respiratory cell and molecular biology

authors： Juárez E,Carranza C,Sánchez G,González M,Chávez J,Sarabia C,Torres M,Sada E

更新日期：2016-12-01 00:00:00

abstract：:Thy-1 is a glycosylphosphytidylinositol-linked cell-surface glycoprotein present on a subset of lung fibroblasts, which plays an important role in postnatal alveolarization. In the present study, we define the role of Thy-1 in pulmonary lipofibroblast differentiation and in the regulation of lipid homeostasis via pero...

journal_title：American journal of respiratory cell and molecular biology

authors： Varisco BM,Ambalavanan N,Whitsett JA,Hagood JS

更新日期：2012-06-01 00:00:00

abstract：:Semaphorins are a large family of proteins originally identified as axon guidance cues that play a crucial role in neural development. They are also ubiquitously expressed beyond the nervous system and contribute to regulation of essential cell functions, such as cell migration, proliferation, and adhesion. Binding of...

journal_title：American journal of respiratory cell and molecular biology

authors： Movassagh H,Khadem F,Gounni AS

更新日期：2018-01-01 00:00:00

abstract：:Pneumocystis carinii causes severe pneumonia in immunocompromised patients. Recent studies indicate that P. carinii uses a Cdc2 cyclin-dependent kinase to control its proliferation. To further study the regulation of the life cycle of P. carinii, we characterized the P. carinii B-type cyclin termed Cdc13, whose bindin...

journal_title：American journal of respiratory cell and molecular biology

authors： Kottom TJ,Thomas CF Jr,Mubarak KK,Leof EB,Limper AH

更新日期：2000-06-01 00:00:00

abstract：:Macrophages are the primary lung phagocyte and are instrumental in maintenance of a sterile, noninflamed microenvironment. IFNs are produced in response to bacterial and viral infection, and activate the macrophage to efficiently counteract and remove pathogenic invaders. Respiratory syncytial virus (RSV) inhibits IFN...

journal_title：American journal of respiratory cell and molecular biology

authors： Senft AP,Taylor RH,Lei W,Campbell SA,Tipper JL,Martinez MJ,Witt TL,Clay CC,Harrod KS

更新日期：2010-04-01 00:00:00

abstract：:Although nitric oxide (NO) is known to inhibit vascular smooth muscle cell proliferation, the subcellular molecular mechanisms involved with the inhibitory signal transduction pathways are uncertain. We investigated the effect of exogenous NO on cell proliferation and the expression of p53, p21, and phosphorylated p42...

journal_title：American journal of respiratory cell and molecular biology

authors： Mizuno S,Kadowaki M,Demura Y,Ameshima S,Miyamori I,Ishizaki T

更新日期：2004-08-01 00:00:00

abstract：:Acute lung injury (ALI) is characterized by pulmonary inflammation and edema. Innate immune cells (e.g., neutrophils and macrophages) are major contributors to inflammation in ALI. Less is known regarding the role of T cells. We examined the effects of rapamycin on inflammation in a LPS-induced murine model of ALI. Ra...

journal_title：American journal of respiratory cell and molecular biology

authors： Nakajima T,Lin KW,Li J,McGee HS,Kwan JM,Perkins DL,Finn PW

更新日期：2014-08-01 00:00:00

abstract：:Primary hamster tracheal surface epithelial (HTSE) cells carry mucin-like glycoproteins on the apical surface which are releasable by neutrophil elastase. In some cancer cells, mucins are localized on the cell surface and have been shown to be encoded by the MUC1 mucin gene. The objectives of the present experiments w...

journal_title：American journal of respiratory cell and molecular biology

authors： Park H,Hyun SW,Kim KC

更新日期：1996-08-01 00:00:00

abstract：:Tumor necrosis factor-alpha (TNF), interleukin-1 beta (IL-1), interleukin-6 (IL-6), and interleukin-8 (IL-8) are inflammatory cytokines produced by alveolar macrophages (AMs) and implicated in sepsis-related adult respiratory distress syndrome (ARDS). Preliminary findings from clinical trials suggest that aerosolized ...

journal_title：American journal of respiratory cell and molecular biology

authors： Thomassen MJ,Meeker DP,Antal JM,Connors MJ,Wiedemann HP

更新日期：1992-09-01 00:00:00

abstract：:We examined the effects of extracellular ATP on the proliferation and synthesis of DNA by guinea pig alveolar macrophages (AM). AM proliferated spontaneously in vitro, their number doubling in 72 h. Such proliferation was completely inhibited by adding 1 mM ATP to the culture. The inhibition was dose dependent. ATP al...

journal_title：American journal of respiratory cell and molecular biology

authors： Nakanishi M,Kawasaki M,Ogino H,Yoshida M,Yagawa K

更新日期：1994-05-01 00:00:00

abstract：:Rat alveolar type II pneumocytes, in situ, label with Maclura pomifera agglutinin (MPA), a plant lectin that recognizes alpha-galactosyl oligosaccharide residues of glycoproteins and glycolipids. To study the glycoproteins recognized by the lectin, MPA lectin affinity chromatography was used to isolate a novel glycopr...

journal_title：American journal of respiratory cell and molecular biology

authors： Lwebuga-Mukasa JS

更新日期：1991-06-01 00:00:00

abstract：:Tumor necrosis factor (TNF) is an inflammatory cytokine produced by many cell types which may contribute to the pathophysiology of a variety of lung diseases. In this study we have used Ro 45-2081 (a soluble receptor composed of the human p55 TNF receptor and human heavy-chain immunoglobulin G) to explore the role of ...

journal_title：American journal of respiratory cell and molecular biology

authors： Gater PR,Wasserman MA,Paciorek PM,Renzetti LM

更新日期：1996-05-01 00:00:00

abstract：:Wheeze is a common symptom in infants, but not all wheezers develop asthma. Indeed, up to 50% of wheezing children outgrow their symptoms by school age. How to predict if early wheeze will become asthma is still a matter of vivid debate. In this work, we sought to assess the clinical and pathological factors that migh...

journal_title：American journal of respiratory cell and molecular biology

authors： Bonato M,Bazzan E,Snijders D,Tinè M,Biondini D,Turato G,Balestro E,Papi A,Cosio MG,Barbato A,Baraldo S,Saetta M

更新日期：2018-10-01 00:00:00

abstract：:Recent in vitro studies have suggested that interleukin-4 (IL-4) may be involved in the preferential migration of eosinophils into the airways in allergic asthma through its capacity to selectively increase vascular cell adhesion molecule-1 (VCAM-1) expression on vessels. To test this hypothesis, we studied the expres...

journal_title：American journal of respiratory cell and molecular biology

authors： Fukuda T,Fukushima Y,Numao T,Ando N,Arima M,Nakajima H,Sagara H,Adachi T,Motojima S,Makino S

更新日期：1996-01-01 00:00:00

abstract：:In the present study, we sought to elucidate the mechanisms by which monocytes migrate into the pleural space in the presence of anaphylatoxins in tuberculous pleural effusion (TPE). Monocytes in both pleural effusion and blood were counted, and their phenotypic characteristics were analyzed. Activation of the complem...

journal_title：American journal of respiratory cell and molecular biology

authors： Luo L,Li X,Hu X,Hu C,Tang W,Deng S,Feng J

更新日期：2019-04-01 00:00:00

abstract：:Recently, investigators demonstrated associations between fine particulate matter (PM)-associated metals and adverse health effects. Residual oil fly ash (ROFA), a waste product of fossil fuel combustion from boilers, is rich in the transition metals Fe, Ni, and V, and when released as a fugitive particle, is an impor...

journal_title：American journal of respiratory cell and molecular biology

authors： Farraj AK,Hazari MS,Haykal-Coates N,Lamb C,Winsett DW,Ge Y,Ledbetter AD,Carll AP,Bruno M,Ghio A,Costa DL

更新日期：2011-02-01 00:00:00

abstract：:Keratinocyte growth factor (KGF) or fibroblast growth factor (FGF)-7, a peptide produced by stromal cells and in particular by lung mesenchyme, has recently been shown to influence early lung morphogenesis and to be a mitogen for fetal and adult alveolar type II cells. Although contradictory findings have been reporte...

journal_title：American journal of respiratory cell and molecular biology

authors： Chelly N,Mouhieddine-Gueddiche OB,Barlier-Mur AM,Chailley-Heu B,Bourbon JR

更新日期：1999-03-01 00:00:00

abstract：:Interleukin-13 (IL-13) is one of the central mediators for development of airway hyperresponsiveness in asthma. However, its effect on bronchial smooth muscle (BSM) is not well known. Recent studies revealed an involvement of RhoA/Rho-kinase in BSM contraction, and this pathway has now been proposed as a new target fo...

journal_title：American journal of respiratory cell and molecular biology

authors： Chiba Y,Nakazawa S,Todoroki M,Shinozaki K,Sakai H,Misawa M

更新日期：2009-02-01 00:00:00

abstract：:Chronic hypoxia induces proliferation of human pulmonary artery smooth muscle cells (hPASMCs), leading to remodeling and pulmonary hypertension, but the mechanism remains unclear. The present study tested the roles of mitochondrial ATP-sensitive potassium channel (mitoK(ATP)) and mitochondrial membrane potential (Delt...

journal_title：American journal of respiratory cell and molecular biology

authors： Hu HL,Zhang ZX,Chen CS,Cai C,Zhao JP,Wang X

更新日期：2010-06-01 00:00:00

abstract：:Pneumocystis is an important fungal pathogen that causes life-threatening pneumonia in patients with AIDS and malignancy. Lung fungal pathogens are recognized by C-type lectin receptors (CLRs), which bind specific ligands and stimulate innate immune responses. The CLR Dectin-1 was previously shown to mediate immune re...

journal_title：American journal of respiratory cell and molecular biology

authors： Kottom TJ,Hebrink DM,Jenson PE,Marsolek PL,Wüthrich M,Wang H,Klein B,Yamasaki S,Limper AH

更新日期：2018-02-01 00:00:00

abstract：:Proteinase-activated receptor (PAR)-2 is cleaved within its aminoterminal extracellular domain by serine proteinases such as trypsin, unmasking a new aminoterminus starting with the sequence SLIGKV, which binds intramolecularly and activates the receptor. PAR-2 has been reported to be involved in inflammation within t...

journal_title：American journal of respiratory cell and molecular biology

authors： Dulon S,Candé C,Bunnett NW,Hollenberg MD,Chignard M,Pidard D

更新日期：2003-03-01 00:00:00