Comparison of protein immunoprecipitation-multiple reaction monitoring with ELISA for assay of biomarker candidates in plasma.

abstract：:Lysine methylation plays important roles in structural and functional regulation of chromatin. Although trypsin is the most widely used protease in mass spectrometry-based proteomic analysis for lysine methylation substrates, the proteolytic activity of trypsin on dimethylated lysine residues remains an arguable issue...

journal_title：Journal of proteome research

authors： Chen M,Zhang M,Zhai L,Hu H,Liu P,Tan M

更新日期：2017-09-01 00:00:00

abstract：:Method development is one of the objectives of the astrophysical community for characterizing the organic matter in objects of the solar system. In this context, we report on the development of an enzyme-catalyzed stereoselective hydrolysis, inspired by the proteomics discipline, which has enabled the indirect detecti...

journal_title：Journal of proteome research

authors： Lange J,Djago F,Eddhif B,Remaury QB,Ruf A,Leitner NKV,Hendecourt LLS,Danger G,Rodier CG,Papot S,Poinot P

更新日期：2020-10-20 00:00:00

abstract：:Tyrosine phosphorylation plays a major role in regulating cell signaling pathways governing diverse biological functions such as proliferation and differentiation. Systemically mapping phosphotyrosine (pTyr) sites is the key to understanding molecular mechanisms underlining pTyr-dependent signaling. Although mass spec...

journal_title：Journal of proteome research

authors： Yao Y,Wang Y,Wang S,Liu X,Liu Z,Li Y,Fang Z,Mao J,Zheng Y,Ye M

更新日期：2019-04-05 00:00:00

abstract：:TGFBR2 serves as an initial regulator of the TGF-β signaling pathway, and loss or reduction of its expression can lead to uncontrolled cell growth. This study was conducted to further explore the mechanism of TGFBR2/SMAD4 on the migration and proliferation of CC cells. Here, TGFBR2 and SMAD4 expressions in CC cells an...

journal_title：Journal of proteome research

authors： Yuan J,Yi K,Yang L

更新日期：2020-08-07 00:00:00

abstract：:When analyzing proteins in complex samples using tandem mass spectrometry of peptides generated by proteolysis, the inference of proteins can be ambiguous, even with well-validated peptides. Unresolved questions include whether to show all possible proteins vs a minimal list, what to do when proteins are inferred ambi...

journal_title：Journal of proteome research

authors： Meyer-Arendt K,Old WM,Houel S,Renganathan K,Eichelberger B,Resing KA,Ahn NG

更新日期：2011-07-01 00:00:00

abstract：:Circulating microvesicles are able to mediate long-distance cell-cell communications. It is essential to understand how microvesicles from pancreatic cancer act on other cells in the body. In this work, serum-derived microvesicles were isolated from 10 patients with locally advanced pancreatic cancer and healthy contr...

journal_title：Journal of proteome research

authors： An M,Zhu J,Wu J,Cuneo KC,Lubman DM

更新日期：2018-04-06 00:00:00

abstract：:We describe a dual-column interface for parallel chromatography to improve throughput during LC-MS experimentation. The system employs a high-voltage switch to operate two capillary column/nanospray emitters fixed at the MS orifice. Sequentially loading one column while operating the second nearly doubles the LC-MS du...

journal_title：Journal of proteome research

authors： Orton DJ,Wall MJ,Doucette AA

更新日期：2013-12-06 00:00:00

abstract：:Acute kidney injury (AKI) is a frequent complication after cardiopulmonary bypass, but early detection of postoperative AKI remains challenging. Protein biomarkers predict AKI excellently in homogeneous cohorts but are less reliable in patients suffering from various comorbidities. We employed nuclear magnetic resonan...

journal_title：Journal of proteome research

authors： Zacharias HU,Hochrein J,Vogl FC,Schley G,Mayer F,Jeleazcov C,Eckardt KU,Willam C,Oefner PJ,Gronwald W

更新日期：2015-07-02 00:00:00

abstract：:The low selectivity of Kv1 peptide inhibitors for specific isoforms makes them poor candidates for the development of theraputics. Using combined approaches, we showed that the Kv1 turret is the critical determinant for ADWX-1 peptide inhibitor selectivity of Kv1.3 over Kv1.1. Mutation of Kv1.1 turret residues to matc...

journal_title：Journal of proteome research

authors： Yin SJ,Jiang L,Yi H,Han S,Yang DW,Liu ML,Liu H,Cao ZJ,Wu YL,Li WX

更新日期：2008-11-01 00:00:00

abstract：:We investigated possible associations between sequence evolution of mammalian sperm proteins and their phosphorylation status in humans. As a reference, spermatozoa from three normozoospermic men were analyzed combining two-dimensional gel electrophoresis, immunoblotting, and mass spectrometry. We identified 99 sperm ...

journal_title：Journal of proteome research

authors： Schumacher J,Ramljak S,Asif AR,Schaffrath M,Zischler H,Herlyn H

更新日期：2013-12-06 00:00:00

abstract：:Protein post-translational modification by ubiquitin-fold modifier 1, UFM1, regulates many biological processes such as response to endoplasmic reticulum stress and regulation of tumor progression. A recent study has indicated that the UFM1-binding and PCI domain-containing protein 1 (UFBP1) is required for the conjug...

journal_title：Journal of proteome research

authors： Zhu Y,Lei Q,Li D,Zhang Y,Jiang X,Hu Z,Xu G

更新日期：2018-04-06 00:00:00

abstract：:The enormous challenges of mass spectrometry-based metaproteomics are primarily related to the analysis and interpretation of the acquired data. This includes reliable identification of mass spectra and the meaningful integration of taxonomic and functional meta-information from samples containing hundreds of unknown ...

journal_title：Journal of proteome research

authors： Muth T,Behne A,Heyer R,Kohrs F,Benndorf D,Hoffmann M,Lehtevä M,Reichl U,Martens L,Rapp E

更新日期：2015-03-06 00:00:00

abstract：:The Human Proteome Project (HPP) has made great efforts to clarify the existing evidence of human proteins since 2012. However, according to the recent release of neXtProt (2019-1), approximately 10% of all human genes still have inadequate or no experimental evidence of their translation at the protein level. They we...

journal_title：Journal of proteome research

authors： Elguoshy A,Hirao Y,Yamamoto K,Xu B,Kinoshita N,Mitsui T,Yamamoto T

更新日期：2019-12-06 00:00:00

abstract：:Rhizobia, the nitrogen-fixing bacterial symbionts of legumes, represent an agricultural application of primary relevance and a model of plant-microbe molecular dialogues. We recently described rhizobium proteome alterations induced by plant flavonoids using iTRAQ. Herein, we further extend that experimentation, provin...

journal_title：Journal of proteome research

authors： Arrigoni G,Tolin S,Moscatiello R,Masi A,Navazio L,Squartini A

更新日期：2013-11-01 00:00:00

abstract：:Coronary artery disease is one of the most frequent causes of morbidity and mortality worldwide. It is even more prevalent in patients with type 2 diabetes mellitus who suffer from obesity and increased accumulation of epicardial fat with a possible contributing role in the development of coronary artery disease. We p...

journal_title：Journal of proteome research

authors： Tomášová P,Čermáková M,Pelantová H,Vecka M,Kratochvílová H,Lipš M,Lindner J,Ivák P,Netuka I,Šedivá B,Haluzík M,Kuzma M

更新日期：2020-10-02 00:00:00

abstract：:MS-based de novo peptide sequencing has been improved remarkably with significant development of mass-spectrometry and computational approaches but still lacks quality-control methods. Here we proposed a novel algorithm pSite to evaluate the confidence of each amino acid rather than the full-length peptides obtained b...

journal_title：Journal of proteome research

authors： Yang H,Chi H,Zhou WJ,Zeng WF,Liu C,Wang RM,Wang ZW,Niu XN,Chen ZL,He SM

更新日期：2018-01-05 00:00:00

abstract：:During the development of cancer, changes in cellular glycosylation are observed, indicating that alterations of the glycome occur in extracellular fluids as well as in serum and could therefore serve as tumor biomarkers. In the case of epithelial ovarian cancer (EOC), common tumor markers such as CA125 are known to h...

journal_title：Journal of proteome research

authors： Biskup K,Braicu EI,Sehouli J,Fotopoulou C,Tauber R,Berger M,Blanchard V

更新日期：2013-09-06 00:00:00

abstract：:Direct tissue profiling and imaging mass spectrometry (MS) provides a detailed assessment of the complex protein pattern within a tissue sample. MALDI MS analysis of thin tissue sections results in over of 500 individual protein signals in the mass range of 2 to 70 kDa that directly correlate with protein composition ...

journal_title：Journal of proteome research

authors： Chaurand P,Schwartz SA,Caprioli RM

更新日期：2004-03-01 00:00:00

abstract：:Crucial metabolites that modulate hosts' metabolome to eliminate bacterial pathogens have been documented, but the metabolic mechanisms are largely unknown. The present study explores the metabolic mechanism for l-leucine-induced metabolome to eliminate Streptococcus iniae in tilapia. GC-MS-based metabolomics was used...

journal_title：Journal of proteome research

authors： Du CC,Yang MJ,Li MY,Yang J,Peng B,Li H,Peng XX

更新日期：2017-05-05 00:00:00

abstract：:There are three quantitative phosphoproteomic strategies most commonly used to study receptor tyrosine kinase (RTK) signaling. These strategies quantify changes in: (1) all three forms of phosphosites (phosphoserine, phosphothreonine and phosphotyrosine) following enrichment of phosphopeptides by titanium dioxide or i...

journal_title：Journal of proteome research

authors： Zhang G,Neubert TA

更新日期：2011-12-02 00:00:00

abstract：:Our previous studies suggest that the α2,3sialylated T-antigen (NeuAcα2,3Galβ1,3GalNac-) and associated glycan structures are likely to be elevated during cancer. An easy and reliable strategy to label mucinous glycans that contain such carbohydrates can enable the identification of novel glycoproteins that are cancer...

journal_title：Journal of proteome research

authors： Chandrasekaran EV,Xue J,Xia J,Locke RD,Patil SA,Neelamegham S,Matta KL

更新日期：2012-04-06 00:00:00

abstract：:FDA oversight of medical devices, including in vitro diagnostic devices (IVDs or laboratory tests), in the United States was a direct result of the passage of the Medical Device Amendments of 1976. This law introduced a series of general controls for medical devices including registration and listing, requirements for...

journal_title：Journal of proteome research

authors： Hackett JL,Gutman SI

更新日期：2005-07-01 00:00:00

abstract：:The paper describes the implementation of a software system based on the Fenyö disulfide bond assignment algorithm. The system allows an investigator to enter data derived from mass spectrum peak assignments, a target protein sequence and other experimental conditions. The output of the system is the set of disulfide ...

journal_title：Journal of proteome research

authors： Craig R,Krokhin O,Wilkins J,Beavis RC

更新日期：2003-11-01 00:00:00

abstract：:The endothelium plays a critical role in orchestrating the inflammatory response seen during sepsis. Many of the inflammatory effects of Gram-negative sepsis are elicited by lipopolysaccharide (LPS), a glycolipid component of bacterial cell walls. Lipid-rich microdomains have been shown to concentrate components of th...

journal_title：Journal of proteome research

authors： Karsan A,Blonder J,Law J,Yaquian E,Lucas DA,Conrads TP,Veenstra T

更新日期：2005-03-01 00:00:00

abstract：:A major source of intestinal metabolites results from both host and microbial processing of dietary nutrients. (1)H NMR-based metabolic profiling of mouse feces was carried out over time in different microbiome mouse models, including conventional (n = 9), conventionalized (n = 10), and "humanized" gnotobiotic mice in...

journal_title：Journal of proteome research

authors： Martin FP,Sprenger N,Montoliu I,Rezzi S,Kochhar S,Nicholson JK

更新日期：2010-10-01 00:00:00

abstract：:Isobaric tagging is the method of choice in mass-spectrometry-based proteomics for comparing several conditions at a time. Despite its multiplexing capabilities, some drawbacks appear when multiple experiments are merged for comparison in large sample-size studies due to the presence of missing values, which result fr...

journal_title：Journal of proteome research

authors： Corthésy J,Theofilatos K,Mavroudi S,Macron C,Cominetti O,Remlawi M,Ferraro F,Núñez Galindo A,Kussmann M,Likothanassis S,Dayon L

更新日期：2018-06-01 00:00:00

abstract：:The impact of low-dose ionizing radiation (IR) on the human brain has recently attracted attention due to the increased use of IR for diagnostic purposes. The aim of this study was to investigate low-dose radiation response in the hippocampus. Female B6C3F1 mice were exposed to total body irradiation with 0 (control),...

journal_title：Journal of proteome research

authors： Hladik D,Dalke C,von Toerne C,Hauck SM,Azimzadeh O,Philipp J,Ung MC,Schlattl H,Rößler U,Graw J,Atkinson MJ,Tapio S

更新日期：2020-01-03 00:00:00

abstract：:Sperm proteomes have emerged for several species; however, the extent of species similarity is unknown. Sheep are an important agricultural species for which a comprehensive sperm proteome has not been produced. In addition, potential proteomic factors from seminal plasma that may contribute to improved fertility afte...

journal_title：Journal of proteome research

authors： Pini T,Leahy T,Soleilhavoup C,Tsikis G,Labas V,Combes-Soia L,Harichaux G,Rickard JP,Druart X,de Graaf SP

更新日期：2016-10-07 00:00:00

abstract：:Protein phosphorylation is a globally adopted and tightly controlled post-translational modification, and represents one of the most important molecular switching mechanisms that govern the entire spectrum of biological processes. In the central nervous system, it has been demonstrated that phosphorylation of key prot...

journal_title：Journal of proteome research

authors： Liao L,McClatchy DB,Park SK,Xu T,Lu B,Yates JR 3rd

更新日期：2008-11-01 00:00:00

abstract：:Protein phosphorylation is a major mechanism of signal transduction in bacteria. Here, we analyzed the proteome and phosphoproteome of a wild-type strain of the food-borne pathogen Listeria monocytogenes that was grown in either chemically defined medium or rich medium containing glucose. We then compared these result...

journal_title：Journal of proteome research

authors： Misra SK,Moussan Désirée Aké F,Wu Z,Milohanic E,Cao TN,Cossart P,Deutscher J,Monnet V,Archambaud C,Henry C

更新日期：2014-12-05 00:00:00