Abstract:
:miRNAs are small RNAs directing many developmental processes by posttranscriptional regulation of protein-coding genes. We uncovered a new role for miR-1-1/133a-2 and miR-1-2/133a-1 clusters in the specification of embryonic cardiomyocytes allowing transition from an immature state characterized by expression of smooth muscle (SM) genes to a more mature fetal phenotype. Concomitant knockout of miR-1-1/133a-2 and miR-1-2/133a-1 released suppression of the transcriptional co-activator myocardin, a major regulator of SM gene expression, but not of its binding partner SRF. Overexpression of myocardin in the embryonic heart essentially recapitulated the miR-1/133a mutant phenotype at the molecular level, arresting embryonic cardiomyocytes in an immature state. Interestingly, the majority of postulated miR-1/133a targets was not altered in double mutant mice, indicating that the ability of miR-1/133a to suppress target molecules strongly depends on the cellular context. Finally, we show that myocardin positively regulates expression of miR-1/133a, thus constituting a negative feedback loop that is essential for early cardiac development.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Wystub K,Besser J,Bachmann A,Boettger T,Braun Tdoi
10.1371/journal.pgen.1003793subject
Has Abstractpub_date
2013-01-01 00:00:00pages
e1003793issue
9eissn
1553-7390issn
1553-7404pii
PGENETICS-D-13-00833journal_volume
9pub_type
杂志文章相关文献
PLoS Genetics文献大全abstract::The heterochromatin-enriched HP1 proteins play a critical role in regulation of transcription. These proteins contain two related domains known as the chromo- and the chromoshadow-domain. The chromo-domain binds histone H3 tails methylated on lysine 9. However, in vivo and in vitro experiments have shown that the affi...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000769
更新日期:2009-12-01 00:00:00
abstract::Allele age has long been a focus of population genetic research, primarily because it can be an important clue to the fitness effects of an allele. By virtue of their effects on fitness, alleles under directional selection are expected to be younger than neutral alleles of the same frequency. We developed a new coales...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008340
更新日期:2019-08-19 00:00:00
abstract::Genetic background exerts a strong modulatory effect on the toxicity of aggregation-prone proteins in conformational diseases. In addition to influencing the misfolding and aggregation behavior of the mutant proteins, polymorphisms in putative modifier genes may affect the molecular processes leading to the disease ph...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000399
更新日期:2009-03-01 00:00:00
abstract::Patients with neonatal severe hyperparathyroidism (NSHPT) are homozygous for the calcium-sensing receptor (CaR) mutation and have very high circulating PTH, abundant parathyroid hyperplasia, and severe life-threatening hypercalcemia. Mice with homozygous deletion of CaR mimic the syndrome of NSHPT. To determine effect...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002294
更新日期:2011-09-01 00:00:00
abstract::Variation in DNA methylation is being increasingly associated with health and disease outcomes. Although DNA methylation is hypothesized to be a mechanism by which both genetic and non-genetic factors can influence the regulation of gene expression, little is known about the extent to which DNA methylation at specific...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007544
更新日期:2018-08-09 00:00:00
abstract::The oligodendrocyte density is greater and myelin sheaths are thicker in the adult male mouse brain when compared with females. Here, we show that these sex differences emerge during the first 10 postnatal days, precisely at a stage when a late wave of oligodendrocyte progenitor cells arises and starts differentiating...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007049
更新日期:2017-11-06 00:00:00
abstract::Endoreplication is a cell cycle variant that entails cell growth and periodic genome duplication without cell division, and results in large, polyploid cells. Cells switch from mitotic cycles to endoreplication cycles during development, and also in response to conditional stimuli during wound healing, regeneration, a...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008253
更新日期:2019-07-10 00:00:00
abstract::Intragenomic conflicts arise when a genetic element favours its own transmission to the detriment of others. Conflicts over sex chromosome transmission are expected to have influenced genome structure, gene regulation, and speciation. In the mouse, the existence of an intragenomic conflict between X- and Y-linked mult...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002900
更新日期:2012-09-01 00:00:00
abstract::Release factors (RFs) govern the termination phase of protein synthesis. Human mitochondria harbor four different members of the class 1 RF family: RF1Lmt/mtRF1a, RF1mt, C12orf65 and ICT1. The homolog of the essential ICT1 factor is widely distributed in bacteria and organelles and has the peculiar feature in human mi...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004616
更新日期:2014-09-18 00:00:00
abstract::Detection and validation of the RNA degradation signals controlling transcriptome stability are essential steps for understanding how cells regulate gene expression. Here we present complete genomic and biochemical annotations of the signals required for RNA degradation by the dsRNA specific ribonuclease III (Rnt1p) a...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005000
更新日期:2015-02-13 00:00:00
abstract::Jacob, the protein encoded by the Nsmf gene, is involved in synapto-nuclear signaling and docks an N-Methyl-D-Aspartate receptor (NMDAR)-derived signalosome to nuclear target sites like the transcription factor cAMP-response-element-binding protein (CREB). Several reports indicate that mutations in NSMF are related to...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005907
更新日期:2016-03-15 00:00:00
abstract::Significant interest has emerged in mapping genetic susceptibility for complex traits through whole-genome association studies. These studies rely on the extent of association, i.e., linkage disequilibrium (LD), between single nucleotide polymorphisms (SNPs) across the human genome. LD describes the nonrandom associat...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.0020142
更新日期:2006-09-08 00:00:00
abstract::Faithful replication and repair of DNA lesions ensure genome maintenance. During replication in eukaryotic cells, DNA is unwound by the CMG helicase complex, which is composed of three major components: the Cdc45 protein, Mcm2-7, and the GINS complex. The CMG in complex with DNA polymerase epsilon (CMG-E) participates...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008494
更新日期:2019-12-09 00:00:00
abstract::Complete metamorphosis (Holometaboly) is a key innovation that underlies the spectacular success of holometabolous insects. Phylogenetic analyses indicate that Holometabola form a monophyletic group that evolved from ancestors exhibiting hemimetabolous development (Hemimetaboly). However, the nature of the changes und...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006020
更新日期:2016-05-02 00:00:00
abstract::Ribosome assembly cofactors are widely conserved across all domains of life. One such group, the ribosome-associated GTPases (RA-GTPase), act as molecular switches to coordinate ribosome assembly. We previously identified the Staphylococcus aureus RA-GTPase Era as a target for the stringent response alarmone (p)ppGpp,...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008346
更新日期:2019-08-29 00:00:00
abstract::The Unfolded Protein Response (UPR) maintains homeostasis in the endoplasmic reticulum (ER) and defends against ER stress, an underlying factor in various human diseases. During the UPR, numerous genes are activated that sustain and protect the ER. These responses are known to involve the canonical UPR transcription f...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003701
更新日期:2013-01-01 00:00:00
abstract::Mimulus guttatus and M. nasutus are an evolutionary and ecological model sister species pair differentiated by ecology, mating system, and partial reproductive isolation. Despite extensive research on this system, the history of divergence and differentiation in this sister pair is unclear. We present and analyze a po...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004410
更新日期:2014-06-26 00:00:00
abstract::The phenotype of the spontaneous mutant mouse hop-sterile (hop) is characterized by a hopping gait, polydactyly, hydrocephalus, and male sterility. Previous analyses of the hop mouse revealed a deficiency of inner dynein arms in motile cilia and a lack of sperm flagella, potentially accounting for the hydrocephalus an...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004689
更新日期:2014-10-23 00:00:00
abstract::Genomic concepts are based on the assumption that phenotypes arise from the expression of genetic variants. However, the presence of non-Mendelian inheritance patterns provides a direct challenge to this view and suggests an important role for alternative mechanisms of gene regulation and inheritance. Over the past fe...
journal_title:PLoS genetics
pub_type: 杂志文章,评审
doi:10.1371/journal.pgen.1004296
更新日期:2014-04-17 00:00:00
abstract::More than 5% of alternatively spliced internal exons in the human genome are derived from Alu elements in a process termed exonization. Alus are comprised of two homologous arms separated by an internal polypyrimidine tract (PPT). In most exonizations, splice sites are selected from within the same arm. We hypothesize...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000717
更新日期:2009-11-01 00:00:00
abstract::Hereditary nasal parakeratosis (HNPK), an inherited monogenic autosomal recessive skin disorder, leads to crusts and fissures on the nasal planum of Labrador Retrievers. We performed a genome-wide association study (GWAS) using 13 HNPK cases and 23 controls. We obtained a single strong association signal on chromosome...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003848
更新日期:2013-01-01 00:00:00
abstract::Meiotic cytokinesis influences the fertility and ploidy of gametes. However, limited information is available on the genetic control of meiotic cytokinesis in plants. Here, we identified a rice mutant with low male fertility, defective callose in meiosis 1 (dcm1). The pollen grains of dcm1 are proved to be defective i...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007769
更新日期:2018-11-12 00:00:00
abstract::The Structural Maintenance of Chromosome (SMC) complex, termed cohesin, is essential for sister chromatid cohesion. Cohesin is also important for chromosome condensation, DNA repair, and gene expression. Cohesin is comprised of Scc3, Mcd1, Smc1, and Smc3. Scc3 also binds Pds5 and Wpl1, cohesin-associated proteins that...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005036
更新日期:2015-03-06 00:00:00
abstract::Examination of the human transcriptome reveals higher levels of RNA editing than in any other organism tested to date. This is indicative of extensive double-stranded RNA (dsRNA) formation within the human transcriptome. Most of the editing sites are located in the primate-specific retrotransposed element called Alu. ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000204
更新日期:2008-09-26 00:00:00
abstract::Down syndrome (DS), commonly caused by an extra copy of chromosome 21 (chr21), occurs in approximately one out of 700 live births. Precisely how an extra chr21 causes over 80 clinically defined phenotypes is not yet clear. Reduced representation bisulfite sequencing (RRBS) analysis at single base resolution revealed D...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003515
更新日期:2013-06-01 00:00:00
abstract::Plant resistance (R) proteins provide a robust surveillance system to defend against potential pathogens. Despite their importance in plant innate immunity, relatively few of the approximately 170 R proteins in Arabidopsis have well-characterized resistance specificity. In order to identify the R protein responsible f...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000894
更新日期:2010-04-01 00:00:00
abstract::Zeins, the predominent storage proteins in maize endosperm, are encoded by multiple genes and gene families. However, only a few transcriptional factors for zein gene regulation have been functionally characterized. In this study, a MADS-box protein, namely ZmMADS47, was identified as an Opaque2 (O2) interacting prote...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005991
更新日期:2016-04-14 00:00:00
abstract::Dishevelled (Dvl) proteins are important signaling components of both the canonical beta-catenin/Wnt pathway, which controls cell proliferation and patterning, and the planar cell polarity (PCP) pathway, which coordinates cell polarity within a sheet of cells and also directs convergent extension cell (CE) movements t...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000259
更新日期:2008-11-01 00:00:00
abstract::Translational systems can respond promptly to sudden environmental changes to provide rapid adaptations to environmental stress. Unlike the well-studied translational responses to oxidative stress in eukaryotic systems, little is known regarding how prokaryotes respond rapidly to oxidative stress in terms of translati...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005302
更新日期:2015-06-19 00:00:00
abstract::Chemosensory pheromonal information regulates aggression and reproduction in many species, but how pheromonal signals are transduced to reliably produce behavior is not well understood. Here we demonstrate that the pheromonal signals detected by Gr32a-expressing chemosensory neurons to enhance male aggression are filt...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004356
更新日期:2014-05-22 00:00:00