Abstract:
:Endoreplication is a cell cycle variant that entails cell growth and periodic genome duplication without cell division, and results in large, polyploid cells. Cells switch from mitotic cycles to endoreplication cycles during development, and also in response to conditional stimuli during wound healing, regeneration, aging, and cancer. In this study, we use integrated approaches in Drosophila to determine how mitotic cycles are remodeled into endoreplication cycles, and how similar this remodeling is between induced and developmental endoreplicating cells (iECs and devECs). Our evidence suggests that Cyclin A / CDK directly activates the Myb-MuvB (MMB) complex to induce transcription of a battery of genes required for mitosis, and that repression of CDK activity dampens this MMB mitotic transcriptome to promote endoreplication in both iECs and devECs. iECs and devECs differed, however, in that devECs had reduced expression of E2F1-dependent genes that function in S phase, whereas repression of the MMB transcriptome in iECs was sufficient to induce endoreplication without a reduction in S phase gene expression. Among the MMB regulated genes, knockdown of AurB protein and other subunits of the chromosomal passenger complex (CPC) induced endoreplication, as did knockdown of CPC-regulated cytokinetic, but not kinetochore, proteins. Together, our results indicate that the status of a CycA-Myb-MuvB-AurB network determines the decision to commit to mitosis or switch to endoreplication in both iECs and devECs, and suggest that regulation of different steps of this network may explain the known diversity of polyploid cycle types in development and disease.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Rotelli MD,Policastro RA,Bolling AM,Killion AW,Weinberg AJ,Dixon MJ,Zentner GE,Walczak CE,Lilly MA,Calvi BRdoi
10.1371/journal.pgen.1008253subject
Has Abstractpub_date
2019-07-10 00:00:00pages
e1008253issue
7eissn
1553-7390issn
1553-7404pii
PGENETICS-D-19-00352journal_volume
15pub_type
杂志文章相关文献
PLoS Genetics文献大全abstract::In Saccharomyces cerevisiae, the conserved phosphatase Cdc14 is required for the exit from mitosis. It is anchored on nucleolar chromatin by the Cfi1/Net1 protein until early anaphase, at which time it is released into the nucleoplasm. Two poorly understood, redundant pathways promote Cdc14 release, the FEAR (Cdc four...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000588
更新日期:2009-08-01 00:00:00
abstract::High glucose diets are unhealthy, although the mechanisms by which elevated glucose is harmful to whole animal physiology are not well understood. In Caenorhabditis elegans, high glucose shortens lifespan, while chemically inflicted glucose restriction promotes longevity. We investigated the impact of glucose metaboli...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008982
更新日期:2020-08-25 00:00:00
abstract::The exclusive localization of the histone H3 variant CENP-A to centromeres is essential for accurate chromosome segregation. Ubiquitin-mediated proteolysis helps to ensure that CENP-A does not mislocalize to euchromatin, which can lead to genomic instability. Consistent with this, overexpression of the budding yeast C...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005930
更新日期:2016-03-16 00:00:00
abstract::HSPB7 is a member of the small heat-shock protein (HSPB) family and is expressed in the cardiomyocytes from cardiogenesis onwards. A dramatic increase in HSPB7 is detected in the heart and blood plasma immediately after myocardial infarction. Additionally, several single-nucleotide polymorphisms of HSPB7 have been ide...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006984
更新日期:2017-08-21 00:00:00
abstract::We resequenced and phased 27 kb of DNA within 580 kb of the MHC class II region in 158 population chromosomes, most of which were conserved extended haplotypes (CEHs) of European descent or contained their centromeric fragments. We determined the single nucleotide polymorphism and deletion-insertion polymorphism allel...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004637
更新日期:2014-10-09 00:00:00
abstract::The molecular mechanisms of animal cell osmoregulation are poorly understood. Genetic studies of osmoregulation in yeast have identified mucin-like proteins as critical regulators of osmosensitive signaling and gene expression. Whether mucins play similar roles in higher organisms is not known. Here, we show that muta...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1001267
更新日期:2011-01-06 00:00:00
abstract::The genetic origin of human skin pigmentation remains an open question in biology. Several skin disorders and diseases originate from mutations in conserved pigmentation genes, including albinism, vitiligo, and melanoma. Teleosts possess the capacity to modify their pigmentation to adapt to their environmental backgro...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1009244
更新日期:2020-12-10 00:00:00
abstract::Conjugation is one mechanism for intra- and inter-species horizontal gene transfer among bacteria. Conjugative elements have been instrumental in many bacterial species to face the threat of antibiotics, by allowing them to evolve and adapt to these hostile conditions. Conjugative plasmids are transferred to plasmidle...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1001165
更新日期:2010-10-21 00:00:00
abstract::A variety of pathologies are associated with exposure to supraphysiological concentrations of essential metals and to non-essential metals and metalloids. The molecular mechanisms linking metal exposure to human pathologies have not been clearly defined. To address these gaps in our understanding of the molecular biol...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000053
更新日期:2008-04-25 00:00:00
abstract::Angelman syndrome (AS) is a severe neurodevelopmental disorder caused by maternal deficiency of the imprinted gene UBE3A. Individuals with AS suffer from intellectual disability, speech impairment, and motor dysfunction. Currently there is no cure for the disease. Here, we evaluated the phenotypic effect of activating...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004039
更新日期:2013-01-01 00:00:00
abstract::Structural features of genomes, including the three-dimensional arrangement of DNA in the nucleus, are increasingly seen as key contributors to the regulation of gene expression. However, studies on how genome structure and nuclear organisation influence transcription have so far been limited to a handful of model spe...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007467
更新日期:2018-10-24 00:00:00
abstract::Sleep is a nearly universal behavior that is regulated by diverse environmental stimuli and physiological states. A defining feature of sleep is a homeostatic rebound following deprivation, where animals compensate for lost sleep by increasing sleep duration and/or sleep depth. The fruit fly, Drosophila melanogaster, ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008270
更新日期:2020-03-11 00:00:00
abstract::Plant microRNAs (miRNAs) are critical regulators of gene expression, however little attention has been given to the principles governing miRNA silencing efficacy. Here, we utilize the highly conserved Arabidopsis miR159-MYB33/MYB65 regulatory module to explore these principles. Firstly, we show that perfect central co...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004232
更新日期:2014-03-13 00:00:00
abstract::The genetic impact associated to the Neolithic spread in Europe has been widely debated over the last 20 years. Within this context, ancient DNA studies have provided a more reliable picture by directly analyzing the protagonist populations at different regions in Europe. However, the lack of available data from the o...
journal_title:PLoS genetics
pub_type: 历史文章,杂志文章
doi:10.1371/journal.pgen.1004401
更新日期:2014-06-05 00:00:00
abstract::The vast majority of meiotic recombination events (crossovers (COs) and non-crossovers (NCOs)) cluster in narrow hotspots surrounded by large regions devoid of recombinational activity. Here, using a new molecular approach in plants, called "pollen-typing", we detected and characterized hundreds of CO and NCO molecule...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003922
更新日期:2013-11-01 00:00:00
abstract::DNA methylation is an ancient molecular modification found in most eukaryotes. In plants, DNA methylation is not only critical for transcriptionally silencing transposons, but can also affect phenotype by altering expression of protein coding genes. The extent of its contribution to phenotypic diversity over evolution...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004785
更新日期:2014-11-13 00:00:00
abstract::Single nucleotide polymorphisms (SNPs) on chromosome 9p21 are associated with coronary artery disease, diabetes, and multiple cancers. Risk SNPs are mainly non-coding, suggesting that they influence expression and may act in cis. We examined the association between 56 SNPs in this region and peripheral blood expressio...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000899
更新日期:2010-04-08 00:00:00
abstract::The clinical spectrum of ciliopathies affecting motile cilia spans impaired mucociliary clearance in the respiratory system, laterality defects including heart malformations, infertility and hydrocephalus. Using linkage analysis and whole exome sequencing, we identified two recessive loss-of-function MNS1 mutations in...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007602
更新日期:2018-08-27 00:00:00
abstract::Male breast cancer accounts for approximately 1% of all breast cancer. To date, risk factors for male breast cancer are poorly defined, but certain risk factors and genetic features appear common to both male and female breast cancer. Genome-wide association studies (GWAS) have recently identified common single nucleo...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002290
更新日期:2011-09-01 00:00:00
abstract::Recently, Wu and colleagues [1] proposed two novel statistics for genome-wide interaction analysis using case/control or case-only data. In computer simulations, their proposed case/control statistic outperformed competing approaches, including the fast-epistasis option in PLINK and logistic regression analysis under ...
journal_title:PLoS genetics
pub_type: 评论,杂志文章
doi:10.1371/journal.pgen.1002625
更新日期:2012-01-01 00:00:00
abstract::Teleost fishes, thanks to their rapid evolution of sex determination mechanisms, provide remarkable opportunities to study the formation of sex chromosomes and the mechanisms driving the birth of new master sex determining (MSD) genes. However, the evolutionary interplay between the sex chromosomes and the MSD genes t...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008013
更新日期:2019-08-22 00:00:00
abstract::Reproductive proteins are among the fastest evolving in the proteome, often due to the consequences of positive selection, and their rapid evolution is frequently attributed to a coevolutionary process between interacting female and male proteins. Such a process could leave characteristic signatures at coevolving gene...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000570
更新日期:2009-07-01 00:00:00
abstract::Aversive learning and memories are crucial for animals to avoid previously encountered stressful stimuli and thereby increase their chance of survival. Neuropeptides are essential signaling molecules in the brain and are emerging as important modulators of learned behaviors, but their precise role is not well understo...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007945
更新日期:2019-02-19 00:00:00
abstract::Resection of DNA double-strand break (DSB) ends is generally considered a critical determinant in pathways of DSB repair and genome stability. Unlike for enzymatically induced site-specific DSBs, little is known about processing of random "dirty-ended" DSBs created by DNA damaging agents such as ionizing radiation. He...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000656
更新日期:2009-09-01 00:00:00
abstract::Enterobacter sp. 638 is an endophytic plant growth promoting gamma-proteobacterium that was isolated from the stem of poplar (Populus trichocarpaxdeltoides cv. H11-11), a potentially important biofuel feed stock plant. The Enterobacter sp. 638 genome sequence reveals the presence of a 4,518,712 bp chromosome and a 157...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000943
更新日期:2010-05-13 00:00:00
abstract::Starvation of cells for the DNA building block dTTP is strikingly lethal (thymineless death, TLD), and this effect is observed in all organisms. The phenomenon, discovered some 60 years ago, is widely used to kill cells in anticancer therapies, but many questions regarding the precise underlying mechanisms have remain...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004310
更新日期:2014-05-08 00:00:00
abstract::How do adapting populations navigate the tensions between the costs of gene expression and the benefits of gene products to optimize the levels of many genes at once? Here we combined independently-arising beneficial mutations that altered enzyme levels in the central metabolism of Methylobacterium extorquens to uncov...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004149
更新日期:2014-02-27 00:00:00
abstract::During neurogenesis, transcription factors combinatorially specify neuronal fates and then differentiate subtype identities by inducing subtype-specific gene expression profiles. But how is neuronal subtype identity maintained in mature neurons? Modeling this question in two Drosophila neuronal subtypes (Tv1 and Tv4),...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002501
更新日期:2012-01-01 00:00:00
abstract::Significant interest has emerged in mapping genetic susceptibility for complex traits through whole-genome association studies. These studies rely on the extent of association, i.e., linkage disequilibrium (LD), between single nucleotide polymorphisms (SNPs) across the human genome. LD describes the nonrandom associat...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.0020142
更新日期:2006-09-08 00:00:00
abstract::A crucial step in the development of muscle cells in all metazoan animals is the assembly and anchorage of the sarcomere, the essential repeat unit responsible for muscle contraction. In Caenorhabditis elegans, many of the critical proteins involved in this process have been uncovered through mutational screens focusi...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000537
更新日期:2009-06-01 00:00:00