Abstract:
:The vast majority of meiotic recombination events (crossovers (COs) and non-crossovers (NCOs)) cluster in narrow hotspots surrounded by large regions devoid of recombinational activity. Here, using a new molecular approach in plants, called "pollen-typing", we detected and characterized hundreds of CO and NCO molecules in two different hotspot regions in Arabidopsis thaliana. This analysis revealed that COs are concentrated in regions of a few kilobases where their rates reach up to 50 times the genome average. The hotspots themselves tend to cluster in regions less than 8 kilobases in size with overlapping CO distribution. Non-crossover (NCO) events also occurred in the two hotspots but at very different levels (local CO/NCO ratios of 1/1 and 30/1) and their track lengths were quite small (a few hundred base pairs). We also showed that the ZMM protein MSH4 plays a role in CO formation and somewhat unexpectedly we also found that it is involved in the generation of NCOs but with a different level of effect. Finally, factors acting in cis and in trans appear to shape the rate and distribution of COs at meiotic recombination hotspots.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Drouaud J,Khademian H,Giraut L,Zanni V,Bellalou S,Henderson IR,Falque M,Mézard Cdoi
10.1371/journal.pgen.1003922subject
Has Abstractpub_date
2013-11-01 00:00:00pages
e1003922issue
11eissn
1553-7390issn
1553-7404pii
PGENETICS-D-13-01788journal_volume
9pub_type
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