当前位置： SCI文献检索 > JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY期刊下所有文献 > PKCε activation promotes FGF-2 exocytosis and induces endothelial cell proliferation and sprouting.

PKCε activation promotes FGF-2 exocytosis and induces endothelial cell proliferation and sprouting.

Abstract：

:Protein kinase C epsilon (PKCε) activation controls fibroblast growth factor-2 (FGF-2) angiogenic signaling. Here, we examined the effect of activating PKCε on FGF-2 dependent vascular growth and endothelial activation. ψεRACK, a selective PKCε agonist induces pro-angiogenic responses in endothelial cells, including formation of capillary like structures and cell growth. These effects are mediated by FGF-2 export to the cell membrane, as documented by biotinylation and immunofluorescence, and FGF-2/FGFR1 signaling activation, as attested by ERK1/2-STAT-3 phosphorylation and de novo FGF-2 synthesis. Similarly, vascular endothelial growth factor (VEGF) activates PKCε in endothelial cells, and promotes FGF-2 export and FGF-2/FGFR1 signaling activation. ψεRACK fails to elicit responses in FGF-2(-/-) endothelial cells, and in cells pretreated with methylamine (MeNH2), an exocytosis inhibitor, indicating that both intracellular FGF-2 and its export toward the membrane are required for the ψεRACK activity. In vivo ψεRACK does not induce angiogenesis in the rabbit cornea. However, ψεRACK promotes VEGF angiogenic responses, an effect sustained by endothelial FGF-2 release and synthesis, since anti-FGF-2 antibody strongly attenuates VEGF responses. The results demonstrate that PKCε stimulation promotes angiogenesis and modulates VEGF activity, by inducing FGF-2 release and autocrine signaling.

journal_name

J Mol Cell Cardiol

journal_title

Journal of molecular and cellular cardiology

authors

Monti M,Donnini S,Morbidelli L,Giachetti A,Mochly-Rosen D,Mignatti P,Ziche M

doi

10.1016/j.yjmcc.2013.07.006

subject

Has Abstract

pub_date

2013-10-01 00:00:00

pages

107-17

eissn

0022-2828

issn

1095-8584

pii

S0022-2828(13)00228-9

journal_volume

63

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY文献大全
  • Multiply attenuated, self-inactivating lentiviral vectors efficiently transduce human coronary artery cells in vitro and rat arteries in vivo.

    abstract：:Endothelial cells (ECs) in normal vessels are poorly transducible by retroviral vectors, which require cell division for gene transduction. Among retroviruses, lentiviruses have the unique ability to integrate their genome into the chromatin of nondividing cells. Here we show that multiply attenuated, self-inactivatin...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2004.11.031

    authors： Céfaï D,Simeoni E,Ludunge KM,Driscoll R,von Segesser LK,Kappenberger L,Vassalli G

    更新日期：2005-02-01 00:00:00

  • Dok-5 is involved in cardiomyocyte differentiation through PKB/FOXO3a pathway.

    abstract：:The insulin receptor substrate (IRS) family plays important roles in cellular growth, signaling, and survival in the brain. We identified IRS6/Dok-5, a member of the IRS family, also expressed in heart. Dok-5 expression level significantly increased during cardiomyocyte differentiation of P19CL6 cells. To understand t...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2009.09.015

    authors： Wen J,Xia Q,Wang C,Liu W,Chen Y,Gao J,Gong Y,Yin B,Ke Y,Qiang B,Yuan J,Peng X

    更新日期：2009-12-01 00:00:00

  • Exogenous nitric oxide can trigger a preconditioned state through a free radical mechanism, but endogenous nitric oxide is not a trigger of classical ischemic preconditioning.

    abstract：:Nitric oxide (NO) has been reported to play an important role in the late phase of ischemic preconditioning (PC) in the rabbit heart. However, the role of NO in the early phase of ischemic PC ("classical PC") is controversial. Accordingly, the present study was designed to determine whether NO contributes to the cardi...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.2000.1152

    authors： Nakano A,Liu GS,Heusch G,Downey JM,Cohen MV

    更新日期：2000-07-01 00:00:00

  • Augmentation of arterial endothelial cell expression of the plasminogen activator inhibitor type-1 (PAI-1) gene by proinsulin and insulin in vivo.

    abstract：:Diabetes mellitus is associated with an increased incidence and greater severity of primary atherosclerosis as well as restenosis after angioplasty for reasons not yet clear. We have shown previously that insulin and proinsulin-typically elevated in blood in patients with type II diabetes-increase plasma activity of p...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1998.0719

    authors： Nordt TK,Sawa H,Fujii S,Bode C,Sobel BE

    更新日期：1998-08-01 00:00:00

  • Myocardial dysfunction and ultrastructural alterations mediated by oxygen metabolites.

    abstract：:The direct effect of oxygen metabolites was studied on isolated perfused rat hearts. Superoxide anion (O2-.) and hydrogen peroxide (H2O2) were generated by adding purine (2.3 mM) and purified xanthine oxidase (0.06 U/ml) to Krebs-Henseleit buffer (pH 7.4). Xanthine oxidase was added to the purine-containing perfusate ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(88)90578-0

    authors： Miki S,Ashraf M,Salka S,Sperelakis N

    更新日期：1988-11-01 00:00:00

  • Coronary collateral growth--back to the future.

    abstract：:The coronary collateral circulation is critically important as an adaptation of the heart to prevent the damage from ischemic insults. In their native state, collaterals in the heart would be classified as part of the microcirculation, existing as arterial-arterial anastomotic connections in the range of 30 to 100 μM ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2011.12.006

    authors： Chilian WM,Penn MS,Pung YF,Dong F,Mayorga M,Ohanyan V,Logan S,Yin L

    更新日期：2012-04-01 00:00:00

  • Short-term administration of Nicotinamide Mononucleotide preserves cardiac mitochondrial homeostasis and prevents heart failure.

    abstract：:Heart failure is associated with mitochondrial dysfunction so that restoring or improving mitochondrial health is of therapeutic importance. Recently, reduction in NAD+ levels and NAD+-mediated deacetylase activity has been recognized as negative regulators of mitochondrial function. Using a cardiac specific KLF4 defi...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2017.09.001

    authors： Zhang R,Shen Y,Zhou L,Sangwung P,Fujioka H,Zhang L,Liao X

    更新日期：2017-11-01 00:00:00

  • Hydrophilic bile salt ursodeoxycholic acid protects myocardium against reperfusion injury in a PI3K/Akt dependent pathway.

    abstract：:The opening of mitochondrial permeability transition pore (PTP) during reperfusion injury of heart has been well demonstrated and thus controlling PTP would attenuate the myocardial damage and cell death. Ursodeoxycholic acid (UDCA) is a hydrophilic bile salt and has been shown to prevent apoptosis in hepatocytes by i...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2005.07.014

    authors： Rajesh KG,Suzuki R,Maeda H,Yamamoto M,Yutong X,Sasaguri S

    更新日期：2005-11-01 00:00:00

  • Two cardiac natriuretic peptide genes (atrial natriuretic peptide and brain natriuretic peptide) are organized in tandem in the mouse and human genomes.

    abstract：:Atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP), which act as cardiac hormones, are produced mainly by the atrium and ventricle, respectively, and are involved in body fluid homeostasis and blood pressure control. The ANP and BNP gene expressions are markedly augmented in ventricles of patients wi...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1996.0170

    authors： Tamura N,Ogawa Y,Yasoda A,Itoh H,Saito Y,Nakao K

    更新日期：1996-08-01 00:00:00

  • miR-200c-SUMOylated KLF4 feedback loop acts as a switch in transcriptional programs that control VSMC proliferation.

    abstract：:The regulation of vascular smooth muscle cell (VSMC) proliferation is an important issue because it has major implications for the prevention of pathological vascular conditions. Using microRNA array screen, we found the expression levels of 200 unique miRNAs in hyperplasic tissues. Among them, miR-200c expression sub...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2015.03.011

    authors： Zheng B,Bernier M,Zhang XH,Suzuki T,Nie CQ,Li YH,Zhang Y,Song LL,Shi HJ,Liu Y,Zheng CY,Wen JK

    更新日期：2015-05-01 00:00:00

  • In vivo colored microspheres in the isolated rat heart for use in NMR.

    abstract：:Myocardial perfusion measurement with colored microspheres may become an alternative for radioactive microsphere techniques. We use and validate a spectrophotometric method that has been previously established for large animals in the isolated perfused rat heart. The perfusion system was adapted for use in a NMR micro...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1996.0053

    authors： Hiller KH,Adami P,Voll S,Roder F,Kowallik P,Bauer WR,Haase A,Ertl G

    更新日期：1996-03-01 00:00:00

  • Detection of TUNEL-positive cardiomyocytes and c-kit-positive progenitor cells in children with congenital heart disease.

    abstract：:The loss of cardiomyocytes by apoptosis and the subsequent replacement by fibrous connective tissues are important features of cardiac remodeling in adult heart disease. In children with CHD, however, the cellular and molecular mechanisms of heart failure have not yet been fully understood because of the anatomical an...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2007.05.011

    authors： Sato H,Shiraishi I,Takamatsu T,Hamaoka K

    更新日期：2007-09-01 00:00:00

  • Neural/Bayes network predictor for inheritable cardiac disease pathogenicity and phenotype.

    abstract：:The cardiac muscle sarcomere contains multiple proteins contributing to contraction energy transduction and its regulation during a heartbeat. Inheritable heart disease mutants affect most of them but none more frequently than the ventricular myosin motor and cardiac myosin binding protein c (mybpc3). These co-localiz...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2018.04.006

    authors： Burghardt TP,Ajtai K

    更新日期：2018-06-01 00:00:00

  • Lysozyme binding to endocardial endothelium mediates myocardial depression by the nitric oxide guanosine 3',5' monophosphate pathway in sepsis.

    abstract：:Inflammatory mediators have been implicated as a cause of reversible myocardial depression in septic shock. We previously reported that the release of lysozyme-c (Lmz-S) from leukocytes from the spleen or other organs contributes to myocardial dysfunction in Escherichia coli septic shock in dogs by binding to a cardia...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2005.06.009

    authors： Mink SN,Bose R,Roberts DE,Jacobs H,Duke K,Bose D,Cheng ZQ,Light RB

    更新日期：2005-10-01 00:00:00

  • Changes in thyroid state affect pHi and Nai+ homeostasis in rat ventricular myocytes.

    abstract：:We have tested the hypothesis that thyroid state may influence both the flow of cellular Ca2+ and the myofilament response to Ca2+ by effects on intracellular pH (pHi) and Na+ (Nai+). Single cardiac myocytes isolated from hypothyroid, euthyroid and hyperthyroid animals were loaded with fura-2/AM (Cai2+ probe), BCECF/A...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1997.0495

    authors： Wolska BM,Averyhart-Fullard V,Omachi A,Stojanović MO,Kallen RG,Solaro RJ

    更新日期：1997-10-01 00:00:00

  • Taurine antagonizes the increase in intracellular calcium concentration induced by alpha-adrenergic stimulation in freshly isolated guinea-pig cardiomyocytes.

    abstract：:The effect of taurine on phenylephrine alpha-adrenergic action was studied in freshly-isolated guinea-pig ventricular myocytes. Intracellular calcium concentration was measured at nearly physiological extracellular CaCl2 in both cell suspension using quin-2 (mean intracellular calcium concentration 154.0 +/- 8.0 nM, n...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(92)93092-x

    authors： Failli P,Fazzini A,Franconi F,Stendardi I,Giotti A

    更新日期：1992-11-01 00:00:00

  • β-Adrenergic induced SR Ca2+ leak is mediated by an Epac-NOS pathway.

    abstract：:Cardiac β-adrenergic receptors (β-AR) and Ca2+-Calmodulin dependent protein kinase (CaMKII) regulate both physiological and pathophysiological Ca2+ signaling. Elevated diastolic Ca2+ leak from the sarcoplasmic reticulum (SR) contributes to contractile dysfunction in heart failure and to arrhythmogenesis. β-AR activati...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2017.04.005

    authors： Pereira L,Bare DJ,Galice S,Shannon TR,Bers DM

    更新日期：2017-07-01 00:00:00

  • Comparison of different iron chelators as protective agents against acute doxorubicin-induced cardiotoxicity.

    abstract：:Doxorubicin (Dox) is a widely used antineoplastic agent. Irreversible cardiomyopathy is a serious and dose-limiting side effect after chronic administration. The iron chelating bispiperazinedione ICRF-187 is currently the only drug which affords protection against Dox-induced cardiotoxicity. To compare the protective ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1994.1136

    authors： Voest EE,van Acker SA,van der Vijgh WJ,van Asbeck BS,Bast A

    更新日期：1994-09-01 00:00:00

  • Cardiomegaly due to myocyte hyperplasia in perinatal rats exposed to 200 ppm carbon monoxide.

    abstract：:Exposure to carbon monoxide (CO) during the fetal and neonatal period was used to evaluate cardiac ventricular regional weight and myocyte growth response to an increased hemodynamic load. Date-mated Sprague-Dawley rats inhaled 200 ppm CO from day 7 of pregnancy until parturition; another group of pregnant rats inhale...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(86)80913-0

    authors： Clubb FJ Jr,Penney DG,Baylerian MS,Bishop SP

    更新日期：1986-05-01 00:00:00

  • Effect of treatment on ventricular function and troponin I proteolysis in reperfused myocardium.

    abstract：:Effects of ischemia time and treatment interventions upon troponin I (TnI) proteolysis and function of reperfused myocardium were examined in isolated, perfused rabbit hearts. Hearts were randomized to 90 min aerobic perfusion, 15 min low-flow (1 ml/min) ischemia (I) and 60 min reperfusion (R) or 60 min low-flow I and...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.2002.1522

    authors： Prasan AM,McCarron HC,Hambly BD,Fermanis GG,Sullivan DR,Jeremy RW

    更新日期：2002-04-01 00:00:00

  • Temperature affects human cardiac sarcoplasmic reticulum energy-mediated calcium transport.

    abstract：:Hypothermic cardioplegic solutions are currently used to preserve cardiac function during transportation. However, it has been shown that end-diastolic compliance decreases in donor hearts during reperfusion. Excessively cold temperatures may affect membrane-bound enzymes (Ca2+ ATPase and Ca2+ uptake) which are necess...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1993.1129

    authors： Labow RS,Hendry PJ,Meek E,Keon WJ

    更新日期：1993-10-01 00:00:00

  • Metabolism of pantothenic acid in hearts of diabetic rats.

    abstract：:The metabolism of pantothenic acid (Pa) by cardiac muscle was studied in normal and diabetic rats. Tissue levels of Coenzyme A (CoA) are elevated in the heart during early (6 to 12 h) diabetes, remains at a high level for several days, and then returns to normal or below normal levels. The increase in total tissue CoA...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(89)90605-6

    authors： Beinlich CJ,Robishaw JD,Neely JR

    更新日期：1989-07-01 00:00:00

  • Targeting endothelial and myocardial dysfunction with tetrahydrobiopterin.

    abstract：:Tetrahydrobiopterin (BH(4)) is an essential cofactor for aromatic amino acid hydroxylases and for all three nitric oxide synthase (NOS) isoforms. It also has a protective role in the cell as an antioxidant and scavenger of reactive nitrogen and oxygen species. Experimental studies in humans and animals demonstrate tha...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2011.03.009

    authors： Moens AL,Kietadisorn R,Lin JY,Kass D

    更新日期：2011-10-01 00:00:00

  • Identification and characterization of proteins in sarcoplasmic reticulum from normal and failing human left ventricles.

    abstract：:Monoclonal and polyclonal antibodies to the major sarcoplasmic reticulum proteins of rabbit skeletal and canine cardiac muscle have been used to identify and characterize the corresponding components of human cardiac sarcoplasmic reticulum. The Ca2(+)-transporting ATPase of human cardiac sarcoplasmic reticulum was ide...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(90)90990-j

    authors： Movsesian MA,Leveille C,Krall J,Colyer J,Wang JH,Campbell KP

    更新日期：1990-12-01 00:00:00

  • Calpain mediates cardiac troponin degradation and contractile dysfunction in atrial fibrillation.

    abstract：:The self-perpetuation of atrial fibrillation (AF) is associated with atrial remodeling, including the degradation of the myofibril structure (myolysis). Myolysis is related to AF-induced activation of cysteine proteases and underlies loss of contractile function. In this study, we investigated which proteases are invo...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2008.08.012

    authors： Ke L,Qi XY,Dijkhuis AJ,Chartier D,Nattel S,Henning RH,Kampinga HH,Brundel BJ

    更新日期：2008-11-01 00:00:00

  • Extracellular matrix remodeling in atrial fibrosis: mechanisms and implications in atrial fibrillation.

    abstract：:Atrial fibrosis has been strongly associated with the presence of heart diseases/arrhythmias, including congestive heart failure (CHF) and atrial fibrillation (AF). Inducibility of AF as a result of atrial fibrosis has been the subject of intense recent investigation since it is the most commonly encountered arrhythmi...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2009.09.001

    authors： Pellman J,Lyon RC,Sheikh F

    更新日期：2010-03-01 00:00:00

  • 'Calcium paradox' in the heart is modulated by cell sodium during the calcium-free period.

    abstract：:We hypothesized that after a Ca2+-free period the magnitude of the Na+ gradient at the onset of Ca2+ reperfusion would grade the ensuing cell Ca2+ gain. Rabbit interventricular septa perfused with Hepes buffered solution (pH 7.4, [Ca2+] = 1.0 mM) and stimulated to contract isometrically at 60 min-1 at 30 degrees C wer...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(84)80002-4

    authors： Ruaño-Arroyo G,Gerstenblith G,Lakatta EG

    更新日期：1984-09-01 00:00:00

  • Protection of energy status of hypoxic cardiomyocytes by mild acidosis.

    abstract：:The question was investigated whether mild acidosis can provide energetic protection on the hypoxic myocardial cell by mechanisms other than negative inotropy. The experimental model used was quiescent ventricular cardiomyocytes isolated from the adult rat. The extracellular pH (pHe) in modified hypoxic Tyrode's solut...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(92)91159-3

    authors： Koop A,Piper HM

    更新日期：1992-01-01 00:00:00

  • Effects of endurance training on cholinergic and adrenergic receptors of rat heart.

    abstract：:To test the hypothesis that alterations in adrenergic or cholinergic receptors occur in response to physical training, and that changes in receptor properties could be mechanistically important in producting the altered cardiovascular physiology of the trained state, we studied the effects of endurance training by swi...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(84)80611-2

    authors： Williams RS,Schaible TF,Bishop T,Morey M

    更新日期：1984-05-01 00:00:00

  • Targeting the ACE2-Ang-(1-7) pathway in cardiac fibroblasts to treat cardiac remodeling and heart failure.

    abstract：:Fibroblasts play a pivotal role in cardiac remodeling and the development of heart failure through the deposition of extra-cellular matrix (ECM) proteins and also by affecting cardiomyocyte growth and function. The renin-angiotensin system (RAS) is a key regulator of the cardiovascular system in health and disease and...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2010.12.003

    authors： Iwata M,Cowling RT,Yeo SJ,Greenberg B

    更新日期：2011-10-01 00:00:00