Abstract:
:The complement system plays an important role in innate and adaptive immunity, which suggests that complement activation could be exploited as a potential strategy for vaccine adjuvants. Here we explored the potential of chitosan-based microparticles (CS-NH2 MPs) as a vaccine adjuvant with an active surface for complement activation due to the abundance of amino groups. In vaccination studies, using recombinant anthrax protective antigen as a model antigen, compared with the control microparticles (amino-cross-linked MPs), we found that microparticles (MPs) with abundant amino groups significantly enhanced higher antigen-specific IgG titers in vivo and enhanced the production of IL-4 and IFN-γ with ex vivo restimulation. Furthermore, proliferative responses of splenocytes to ex vivo antigen restimulation were enhanced following immunization with MPs with amino groups. Overall, these results indicated that CS-NH2 MPs with a high surface density of amino groups were favorable for complement activation and immune responses. Our data provide further design principles for studies on complement-activating MPs as a vaccine platform.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Liu Y,Yin Y,Wang L,Zhang W,Chen X,Yang X,Xu J,Ma Gdoi
10.1021/bm400930ksubject
Has Abstractpub_date
2013-09-09 00:00:00pages
3321-8issue
9eissn
1525-7797issn
1526-4602journal_volume
14pub_type
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