Abstract：
OBJECTIVES:To determine the phenotypic and molecular characteristics of Enterococcus faecalis recovered from primary endodontic infections in Brazilian patients. METHODS:Twenty isolates of E. faecalis recovered from 43 Brazilian patients with primary endodontic infections were identified by biochemical profiling (API20Strep) and 16S rDNA sequencing. Antimicrobial susceptibility was ascertained by agar dilution, using the recommended protocol of the Clinical and Laboratory Standards Institute (CLSI). PCR with validated primers was used to detect genes associated with antibiotic resistance and specific virulence factors. RESULTS:All isolates were deemed susceptible to penicillin G, erythromycin and vancomycin. However, nine isolates had a minimum inhibitory concentration of 4μg/mL to vancomycin (the resistance breakpoint). Fourteen isolates (70% of isolates) were also resistant to tetracycline with MICs of >64μg/mL. PCR products for tetracycline resistance genes were detected in test isolates, while erythromycin and vancomycin resistance genes were not evident. Gelatinase, aggregation substance and enteroccocal surface protein genes were detected in 20, 18 and 12 isolates, respectively. CONCLUSIONS:Endodontic E. faecalis isolates exhibit high level of resistance to tetracycline, an antibiotic that has use in local treatment of dental infections. This opens up a much-needed debate on the role and efficacy of this antibiotic for oral infections. Furthermore, these isolates were shown to possess genes that could contribute to pathogenicity in the pulp cavity.
journal_name
J Dentjournal_title
Journal of dentistryauthors
Lins RX,de Oliveira Andrade A,Hirata Junior R,Wilson MJ,Lewis MA,Williams DW,Fidel RAdoi
10.1016/j.jdent.2013.07.004subject
Has Abstractpub_date
2013-09-01 00:00:00pages
779-86issue
9eissn
0300-5712issn
1879-176Xpii
S0300-5712(13)00173-5journal_volume
41pub_type
杂志文章abstract：AIM:It is the aim of this paper to investigate the survival of direct-placement restorations provided within the General Dental Services in England and Wales, in relation to the dentist factors which may affect this. METHODS:For this work, survival of a restoration was considered to be the time between the date of com...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2005.03.009
更新日期：2005-11-01 00:00:00
abstract：OBJECTIVES:Root canal treatment forms an essential part of general dental practice. Sodium hypochlorite (NaOCl) is the most commonly used irrigant in endodontics due to its ability to dissolve organic soft tissues in the root canal system and its action as a potent antimicrobial agent. Although NaOCl accidents created ...
journal_title：Journal of dentistry
pub_type： 杂志文章,评审
doi：10.1016/j.jdent.2013.08.012
更新日期：2013-11-01 00:00:00
abstract：OBJECTIVES:To determine the influence of two enamel conditioning techniques on buccal and lingual tooth surfaces at two different times on debonding strength and tooth damage. METHODS:The study included 50 premolars. Buccal and lingual surfaces of 10 teeth were scanned using SEM before (N=4) and after enamel condition...
journal_title：Journal of dentistry
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.jdent.2004.08.005
更新日期：2005-02-01 00:00:00
abstract：OBJECTIVE:To compare near infrared transillumination device, DIAGNOcam (DC) and bitewing radiography (BW) for the detection of proximal caries. MATERIALS AND METHODS:This retrospective analysis of DC and BW images of 18 students in dental medicine who had consented to the anonymous use of their dental record. The data...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2017.12.008
更新日期：2018-03-01 00:00:00
abstract：OBJECTIVE:To investigate the remineralisation of chitosan pre-treated enamel white spot lesions (WSLs) by bioglass in the presence of the pellicle layer. METHODS:50 artificial enamel white spot lesions were created by acidic gel. Two lesions were used to investigate the formation of the pellicle layer by treating with...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2018.02.004
更新日期：2018-05-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to measure the satisfaction of patients with respect to the aesthetics of veneer restorations (VRs) and to identify potential factors influencing their satisfaction. MATERIALS AND METHODS:One hundred and eighty VRs of three different types (direct composite, indirect composite and ...
journal_title：Journal of dentistry
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0300-5712(96)00067-x
更新日期：1997-11-01 00:00:00
abstract：OBJECTIVES:The bonding of acrylic resin to dental prostheses constructed from metal has been the subject of extensive research in recent years, much of it between base-metal alloys such as Ni-Cr alloys and dental composite. The development of techniques for the manufacture of dental appliances from titanium and titaniu...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/s0300-5712(96)00051-6
更新日期：1997-09-01 00:00:00
abstract：INTRODUCTION:We assessed time trends in root caries experience, i.e. the sum of filled and carious root surfaces (FRS, CRS), and evaluated risk indicators of FRS/CRS in Germany. METHODS:FRS and CRS from repeated waves (1997, 2005, 2014) of the nationally-representative German Oral Health Studies were analyzed in 35-44...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2018.08.013
更新日期：2018-11-01 00:00:00
abstract：OBJECTIVES:To investigate the influence of pre-heating of high-viscosity bulk-fill composite materials on their degree of conversion and shrinkage force formation. METHODS:Four bulk-fill composite materials (Tetric EvoCeram Bulk Fill-TECBF, x-tra fil-XF, QuixFil-QF, SonicFill-SF) and one conventional nano-hybrid resin...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2015.07.014
更新日期：2015-11-01 00:00:00
abstract：OBJECTIVES:The in-vitro dissolution of bioactive glass-based toothpastes and their capacity to form apatite-like phases in buffer solutions have been investigated. MATERIALS AND METHODS:The commercial toothpaste samples were tested on immersion in artificial saliva, Earle's salt solution and Tris buffer for duration f...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2017.10.015
更新日期：2018-01-01 00:00:00
abstract：OBJECTIVES:The aim of the current study was to evaluate the effect of 2% CHX and 2% CHX-methacrylate compared to the resin-dentin bonds created by a two-step etch-and-rinse adhesive system after 24h, 6min and 12min. METHODS:Microtensile bond strengths and interfacial nanoleakage within resin-dentin interfaces created ...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2015.11.002
更新日期：2016-02-01 00:00:00
abstract：OBJECTIVES:To determine the perceptibility and acceptability of tooth color differences using computer-generated pairs of teeth with simulated gingival displayed on a calibrated color monitor using appropriate signal detection theory methodology (SDT). METHODS:Twelve dental professionals (four from each of the followi...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2007.03.006
更新日期：2007-07-01 00:00:00
abstract：OBJECTIVES:To evaluate colour differences between (1) CAD-CAM ceramic systems considering shades A1, A2 and A3 and the corresponding nominal shade of VC (Vita Classical shade guide) and (2) shades A1-A2, A2-A3 and A1, A2 and A3 within the same ceramic system. METHODS:Samples of shades A1, A2 and A3 were fabricated (n=...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2015.02.015
更新日期：2015-06-01 00:00:00
abstract：OBJECTIVES:To evaluate the incidence of prosthetic complications in implant-retained crowns made with UCLA castable abutments and to identify possible risk factors with a view to establishing recommendations to help predict the success of such restorations. METHODS:A cohort follow-up study was carried out in 71 partia...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2012.09.001
更新日期：2012-12-01 00:00:00
abstract：OBJECTIVES:Inhibition of hybrid layer degradation, for example via inhibition of matrix-metallo-proteinases (MMP) could reduce risk of retention loss and failure of adhesively placed restorations. This systematic review investigated such inhibitory pretreatment qualitatively and via meta- and trial-sequential-analysis....
journal_title：Journal of dentistry
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.jdent.2016.04.007
更新日期：2016-06-01 00:00:00
abstract：OBJECTIVES:(1) To systematically review the clinical application and remineralization potentials of ozone in dentistry; (2) To summarize the available in vitro applications of ozone in dentistry. SOURCES:Ovid MEDLINE, CINAHL, etc. (up to April 2007). STUDY SELECTION:In vitro or in vivo English language publications, ...
journal_title：Journal of dentistry
pub_type： 杂志文章,评审
doi：10.1016/j.jdent.2007.11.008
更新日期：2008-02-01 00:00:00
abstract：OBJECTIVES:To determine the correlation between fracture toughness and leucite content in dental porcelains. The mechanisms by which leucite influences the fracture toughness of dental porcelains were also investigated. METHODS:Six porcelains were tested: A (Ceramco I/Dentsply), B (Ceramco II/Dentsitply), C (Finesse/D...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2005.02.001
更新日期：2005-10-01 00:00:00
abstract：OBJECTIVES:To investigate the effect of impregnation of poly(ethyl methacrylate) and tetrahydro-furfuryl methacrylate (PEM/THFM) polymeric delivery system with chlorhexidine or fluconazole on its shear bond strength (SBS) and water sorption. METHODS:For SBS testing, 16 PEM/THFM discs impregnated with chlorhexidine (CH...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2012.04.027
更新日期：2012-08-01 00:00:00
abstract：OBJECTIVE:This study evaluated the effect of 2% chlorhexidine-containing acid (Ac/CHX) and 2% chlorhexidine digluconate solution (CHX) on immediate (IM) and 2-year (2Y) resin-dentin bond strength (BS) and silver nitrate uptake (SNU) for two simplified etch-and-rinse adhesives. METHODS:Forty-two caries-free extracted m...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2010.10.001
更新日期：2011-01-01 00:00:00
abstract：:Glass-ionomer cements (GIC) have been extensively used in dentistry for over 30 years. Due to their excellent biocompatibility in dental applications GIC have been formulated for medical applications. The past decade has seen some impressive advances in the development of medical GICs, however these advances have been...
journal_title：Journal of dentistry
pub_type： 杂志文章,评审
doi：10.1016/j.jdent.2004.10.027
更新日期：2006-09-01 00:00:00
abstract：OBJECTIVES:To assess the cuspal deflection and cervical microleakage of standardised Class II cavities incrementally filled with a dimethacrylate RBC or bulk-fill flowable RBC bases. METHODS:Twenty-four sound upper premolar teeth with Class II cavities were allocated to three groups (n=8). Restoration of the teeth inv...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2012.02.015
更新日期：2012-06-01 00:00:00
abstract：OBJECTIVES:The aim of the present study is to investigate variables of the past caries experience as indicators of future caries in a Chinese child population. METHODS:Caries was recorded at baseline and 2 years later in 433, 6-7-year-old Chinese children. Correlation coefficients between variables of the past caries ...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2005.12.010
更新日期：2006-10-01 00:00:00
abstract：PURPOSE:Develop a methodology to correlate microtensile bond strength (microTBS) and leakage within the same site of a specimen and evaluate the predominate debond and leakage pathways. MATERIALS AND METHODS:A Class II (MO) slot cavity was prepared in 37 extracted human molars and restored with indirect resin composit...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/s0300-5712(00)00065-8
更新日期：2001-02-01 00:00:00
abstract：OBJECTIVES:This paper reports the findings of a study on the roles of dental nurses in cross-infection control in general dental practices and who were undergoing training for their initial certificate. The research aimed to find out what factors in their vocation gave them satisfaction, what caused stress and the bear...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/0300-5712(95)00094-1
更新日期：1996-09-01 00:00:00
abstract：OBJECTIVE:To study the combined effect of simulated occlusal loading and plaque-derived biofilm on the interfacial integrity of dental composite restorations, and to explore whether the effects are modulated by the incorporation of sucrose. METHODS:MOD-class-II restorations were prepared in third molars. Half of the s...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2017.08.009
更新日期：2017-11-01 00:00:00
abstract：OBJECTIVES:Proanthocyanidins (PACs) are biocompounds mimicking native collagen cross-links. The effective and practical delivery of any biocompound is pivotal for clinical usage. The aim was to investigate the dentin biomodification and effective formation of dentin-resin biointerfaces of two highly bioactive PAC-rich ...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2020.103354
更新日期：2020-08-01 00:00:00
abstract：:A 99.5 per cent pure aluminium step-wedge served as reference for evaluating the radiopacity of 55 anterior and posterior composites. The radiopacity of all materials evaluated was compared with the radiopacity of human enamel and human dentine of equivalent sample thickness. Seventeen composites exhibited a radiopaci...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/0300-5712(91)90056-5
更新日期：1991-12-01 00:00:00
abstract：OBJECTIVES:This study evaluated the immediate and 6-month resin-dentin micro-bond strength (microTBS) of one-step self-etch systems (Adper Prompt L-Pop [AD] 3M ESPE; Xeno III [XE] Dentsply De Trey; iBond [iB] Heraeus Kulzer) under different application modes. MATERIALS AND METHODS:Dentin oclusal surfaces were exposed ...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2008.01.018
更新日期：2008-05-01 00:00:00
abstract：OBJECTIVE:The aim of this long-term follow-up was to collect up to 17-year survival data of different metal post-and-core restorations with a covering crown. METHODS:At initiation of the study, a controlled clinical trial, single tooth was provided with an artificial covering crown, by 18 operators. Restorations under...
journal_title：Journal of dentistry
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.jdent.2007.07.006
更新日期：2007-10-01 00:00:00
abstract：:The effects of sample thickness on the in vitro bond strength of two light-activated glass polyalkenoate cements to dentine have been investigated. The buccal and/or lingual surfaces of non-carious human third molar teeth were used for bonding. The tensile bond strengths to dentine of samples of the cements of 0.5 mm,...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/0300-5712(94)90123-6
更新日期：1994-08-01 00:00:00
