Abstract:
:In order to identify and characterize the sheep major histocompatibility complex (MHC) class II molecules, a panel of 19 monoclonal antibodies (mAb) has been raised following immunization of mice with a variety of class II antigen preparations. Antibodies were selected by ELISA using immunopurified sheep class II as antigen and further screened for the ability to react on immunoblots. Nine mAb reacted with the beta-chain and four reacted with the alpha-chain. The chain specificity of the remainder could not be determined as they did not blot. The anti-alpha and anti-beta mAb all reacted with deglycosylated class II. With the exception of one anti-alpha and one anti-beta antibody, the antibodies reacted with both efferent lymph cells and peripheral blood mononuclear cells (PBMC). Three of the anti-sheep class II mAb reacted with human PBMC.
journal_name
Immunologyjournal_title
Immunologyauthors
Dutia BM,Hopkins J,Allington MP,Bujdoso R,McConnell Isubject
Has Abstractpub_date
1990-05-01 00:00:00pages
27-32issue
1eissn
0019-2805issn
1365-2567journal_volume
70pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::Mice homozygous for the gld (generalized lymphoproliferative disease) and lpr (lymphoproliferation) mutations display similar autoimmune and lymphoproliferative diseases. Both result from defective apoptosis, the targets of the lpr and gld mutations being the genes for, respectively, an apoptosis-signalling receptor [...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00
abstract::The opossum major histocompatibility complex (MHC) shares a similar organization with that of non-mammals while containing a diverse set of class I genes more like that of eutherian (placental) mammals. There are 11 class I loci in the opossum MHC region, seven of which are known to be transcribed. The previously desc...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2008.02994.x
更新日期:2009-09-01 00:00:00
abstract::Rapid expulsion of the nematode Trichinella spiralis from immune rats is associated with an increase in volume of intestinal exudate and the presence of large numbers of tissue mucosal mast cells (MMC) and eosinophils. We have measured the concentrations of leukotrienes (LT) C4 (LTC4) and B4 (LTB4) in gut perfusates a...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1987-03-01 00:00:00
abstract::The mechanisms of action and the nature of the co-operating cells (CC) controlling human T-lymphocyte-colony formation were investigated. Media conditioned by PHA-stimulated blood mononuclear cells (MC) were tested for their capacity to induce T-colony formation in the effluent cell population, obtained after anti (Fa...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1982-02-01 00:00:00
abstract::Monoclonal antibodies (MAb) against sequential or conformational epitopes on foot-and-mouth disease virus (FMDV) passively protected neonatal syngeneic (BALB/c) mice at dilutions at which they could not neutralize virus infectivity in vitro. The B2, D9, 1C6 and 4C9 MAb, against the Group 1 (sequential) and Group 2 (co...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1986-07-01 00:00:00
abstract::The process by which naive T cells become activated, differentiate into effector cells and ultimately generate long-lived memory cells is dependent upon a number of factors, including the costimulatory signals received by the T cell. To best understand the multiple events involved, it is important to understand the po...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2006.02396.x
更新日期:2006-08-01 00:00:00
abstract::Cytokines are the most important inducers of T helper (Th) cell differentiation. Interleukin-12 (IL-12) and interferon-alpha (IFN-alpha) are responsible for human Th1-cell differentiation, while IL-4 is the critical cytokine promoting Th2-cell development. These two subsets of cells co-ordinate immunological responses...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2005.02201.x
更新日期:2005-09-01 00:00:00
abstract::Different kinds of IgM antibodies were tested for their activity in antibody-dependent cellular cytotoxicity (ADCC): firstly an anti-benzylpenicilloyl (BPO) IgM antibody from immune rabbit serum purified by affinity, ion exchange, and molecular-sieving chromatography, secondly two monoclonal rat anti-BPO IgM antibodie...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-05-01 00:00:00
abstract::Two phenotypically distinct subpopulations of dendritic cells (SIRPalpha+ CC81Ag- DC and SIRPalpha- CC81Ag+ DC) have previously been identified in bovine afferent lymph which show functional differences when assayed in vitro. The purpose of this study was to investigate whether differences in cytokine production betwe...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2003.01712.x
更新日期:2003-09-01 00:00:00
abstract::In general, the majority of bacteria are pre-inflammatory when injected in experimental animals. However, Mycobacterium leprae has no inflammatory effect when injected into mouse footpad, but using the delipidated mycobacteria we observed a mild significant increase in footpad oedema. Other mycobacteria, Mycobacterium...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1996.d01-786.x
更新日期:1996-12-01 00:00:00
abstract::Antibody-dependent cell-mediated cytotoxicity (ADCC) of infectious bovine rhinotracheitis (IBR)-infected bovine kidney cells (MDBK) by neutrophils was demonstrated. Neutrophils from bovine and sheep mammary exudate and peripheral blood, and also from human peripheral blood, were all active in the presence of anti-IBR ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1984-10-01 00:00:00
abstract::The liver is a front-line immune tissue that plays a major role in the detection, capture and clearance of pathogens and foreign antigens entering the bloodstream, especially from the gut. Our largest internal organ maintains this immune barrier in the face of constant exposure to external but harmless antigens throug...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/imm.13193
更新日期:2020-06-01 00:00:00
abstract::Simultaneous kinetic measurements have been made of basophil degranulation and of histamine release. Both antigen and ionophore A23187 were studied. Cells from the same donor under the same conditions undergo both reactions at a similar rate. Regression analysis indicated a statistically highly significant correlation...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1980-07-01 00:00:00
abstract::The immunosuppression exhibited by a water-soluble condensate of tobacco smoke (WSC) has been studied in vivo and in vitro. When multiple sublethal doses of WSC were injected into C57Bl/6 mice, their ability to respond to immunization with sheep erythrocytes by the formation of plaque-forming cells was severely inhibi...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::The functional avidity of a cytotoxic T lymphocyte (CTL) is known to be a critical determinant of the efficacy with which it clears pathogens. High avidity cells, which are by definition highly sensitive to peptide antigen, are superior for elimination of viruses and tumours. Our studies have established the ability o...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2011.03440.x
更新日期:2011-07-01 00:00:00
abstract::We and others have established an important role for phosphoinositide-3 kinase gamma (PI3Kgamma) in the chemotactic responses of macrophages and neutrophils. The involvement of this lipid kinase in allergic inflammatory responses is, however, yet to be fully determined. Here we compare wild-type (WT) and PI3Kgamma(-/-...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2008.02908.x
更新日期:2009-03-01 00:00:00
abstract::Crry (CR1-related gene/protein) is a rodent complement regulator that inhibits C3 convertases. CD59 is a conserved protein inhibitor active towards C8 and C9. We have previously produced rat Crry as a recombinant soluble (rs) protein in Pichia pastoris. In this study we produced functionally active rat rsCD59 and a ch...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2000.00945.x
更新日期:2000-01-01 00:00:00
abstract::Two herpes simplex virus type 1 (HSV-1) antigens of apparent molecular weight 123,000 and 63,000 which are associated with the viral glycoprotein complex have been identified and purified using virus-specific monoclonal antibodies. Mice immunized with either glycoprotein showed marked resistance to challenge with viru...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1983-06-01 00:00:00
abstract::We have previously demonstrated that intranasal (i.n.) administration of the major encephalitogenic peptide, Ac1-9 of myelin basic protein (MBP), inhibited T-cell responsiveness in vitro and induced tolerance in the H-2u mouse model of experimental autoimmune encephalomyelitis (EAE). The peptide analogue Ac1-9[4Y] wit...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1999.00795.x
更新日期:1999-06-01 00:00:00
abstract::The pathology of the spleen, lymph nodes and thymus of C57L/J mice, infected intraperitoneally with 20 and 100 cysts of Echinococcus multilocularis is described at 2, 4, 8 and 12 weeks postinfection (p.i.). For the first 8 weeks, growth of the larval cyst mass (LCM) was slow and blastogenesis in T-dependent areas of b...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1978-05-01 00:00:00
abstract::A decrease in the number of dendritic cells (DCs) is a major cause of post-sepsis immunosuppression and opportunistic infection and is closely associated with poor prognosis. Increasing the number of DCs to replenish their numbers post sepsis can improve the condition. This therapeutic approach could improve recovery ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12423
更新日期:2015-05-01 00:00:00
abstract::Testing of extracts of Toxoplasma gondii (RH strain) indicated that delayed hypersensitivity as manifested by delayed skin reactions and inhibition of macrophage migration was induced only by those fractions containing RNA. ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1975-04-01 00:00:00
abstract::The cellular prion protein (PrPc) is a glycosyl-phosphatidylinositol (GPI)-linked cell surface protein, which is expressed at high density on nervous tissues and at lower levels on most other solid-organ tissues. It is also expressed on peripheral blood mononuclear cells (PBMC) of all lineages. In lymphocytes, its lev...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2001.01166.x
更新日期:2001-02-01 00:00:00
abstract::Guinea-pigs immunized with homogenates of unfed larvae and nymphs of the tick Rhipicephalus appendiculatus developed significant levels of protective immunity to infestation with adults of this species. The mean engorged weight of female ticks feeding on immunized animals (181.96 +/- 05.63 mg and 170.11 +/- 11.54 mg) ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1990-09-01 00:00:00
abstract::Synthesis of interferon (IFN)-gamma by natural killer (NK) cells is an important pro-inflammatory event with interleukin (IL)-12 and IL-18 playing major inductive roles. However, other temporal events are likely to regulate such processes and as prostaglandin E2 (PGE2) is ubiquitous during inflammation this study test...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2004.01810.x
更新日期:2004-03-01 00:00:00
abstract::Systemic lupus erythematosus (SLE) is an autoimmune disease that involves dysregulation of B and T cells. A tolerogenic peptide, designated hCDR1, ameliorates disease manifestations in SLE-afflicted mice. In the present study, the effect of treatment with hCDR1 on the CD74/macrophage migration inhibitory factor (MIF) ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2010.03342.x
更新日期:2011-01-01 00:00:00
abstract::The composition of the microbiome in health and disease has only recently become a major research focus. Although it is clear that an imbalance or dysbiosis in the microbiota is associated with disease, its interrelatedness to disease penetrance is largely unknown. Inflammatory bowel disease (IBD) is an excellent dise...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/imm.12511
更新日期:2015-11-01 00:00:00
abstract::Human granulocyte-macrophage colony-stimulating factor (GM-CSF) reporter constructs containing up to 3.3 kb of upstream promoter sequence were transiently transfected into both Jurkat and HUT78 human T-cell lines. In Jurkat cells, stimulation with phorbol 12-myristate 13-acetate (PMA) plus phytohemaglutinin (PHA) prod...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.0019-2805.2004.01833.x
更新日期:2004-04-01 00:00:00
abstract::Initial separation and concentration of Clq from fresh, normal equine serum was accomplished by precipitation in 0.02 M acetate buffer, pH 5.5, at 4 degrees for 24 h. The re-dissolved precipitate was clarified by centrifugation at 80,000 g for 1 h and then dialysed against Tris-HCl buffer (0.05 M, pH 8.0) containing 1...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1979-07-01 00:00:00
abstract::Specific and non-specific IgE antibody responses were studied in SD rats infected with between 5 and 2500 Nippostrongylus brasiliensis (NB) larvae. In rats with 2500 NB larvae, specific IgE antibody, measured by enzyme-linked immunosorbent assay (ELISA) using NB excretory/secretory substance as antigen, reached a peak...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00