Abstract：
:Thioredoxin peroxidase (Tpxs) plays an important role in maintaining redox homeostasis and in protecting organisms from the accumulation of toxic reactive oxygen species (ROS). Here, we isolated a mitochondrial thioredoxin peroxidase gene from Apis cerana cerana, AccTpx-3. The open reading frame (ORF) of AccTpx-3 is 729 bp in length and encodes a predicted protein of 242 amino acids, 27.084 kDa and an isoelectric point of 8.70. Furthermore, the 980 bp 5' flanking region was cloned, and the transcription factor binding sites were predicted. A quantitative RT-PCR (Q-PCR) analysis indicated that AccTpx-3 was expressed higher in muscle than other tissues, with its highest expression occurring on the fourth day of the larval stage, followed by the fifteenth day of the adult stage. Moreover, the expression of the AccTpx-3 transcript was upregulated by such abiotic stresses as 4°C, 42°C, H(2)O(2), cyhalothrin, acaricide and phoxime treatments. In contrast, AccTpx-3 transcription was downregulated by other abiotic stresses, including 16°C, 25°C, ultraviolet light and HgCl(2). Recombinant AccTpx-3 protein acted as a potent antioxidant that resisted paraquat-induced oxidative stress and protected DNA from oxidative damage. Taken together, these results suggest that the AccTpx-3 protein is an antioxidant enzyme that may protect organisms from oxidative stress.
journal_name
J Insect Physioljournal_title
Journal of insect physiologyauthors
Yao P,Lu W,Meng F,Wang X,Xu B,Guo Xdoi
10.1016/j.jinsphys.2012.11.005subject
Has Abstractpub_date
2013-03-01 00:00:00pages
273-82issue
3eissn
0022-1910issn
1879-1611pii
S0022-1910(12)00292-2journal_volume
59pub_type
杂志文章abstract：:Physiological performance and tolerance limits in metazoans have been widely studied and have informed our understanding of processes such as extreme heat and cold tolerance, and resistance to water loss. Because of scaling considerations, very small arthropods with extreme microclimatic niches provide promising extre...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2015.08.002
更新日期：2015-11-01 00:00:00
abstract：:Apolipophorin III (apoLp-III) was isolated from the haemolymph of last instar larvae of Galleria mellonella. The ultraviolet (u.v.) spectrum and the N-terminal amino acid sequence reveal high similarities with the apoLp-III from Manduca sexta. The protein is heat-stable. The molecular mass of apoLp-III was determined ...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(96)00113-8
更新日期：1997-04-01 00:00:00
abstract：:When JH II, III or methoprene was applied in the nymphal stages to two different strains of the brown planthopper which were selected to produce long (macropterous) or short (brachypterous) wing forms, no effect was observed on the molting profile or metamorphosis. Brachypterization of a majority of the presumptive ma...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(98)00103-6
更新日期：1999-01-01 00:00:00
abstract：:Thermal tolerance, supercooling point, water balance and osmoregulatory ability of Pringleophaga marioni Viette (Lepidoptera: Tineidae) are investigated in this study. Field-fresh larvae had a mean CT(Min) (cold stupor) of -0.6 degrees C and a mean CT(Max) (heat coma) of 38.7 degrees C. The mean supercooling point of ...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(97)00001-2
更新日期：1997-07-01 00:00:00
abstract：:The development of genetically modified vectors refractory to parasites is seen as a promising strategy in the future control of endemic diseases such as malaria. Nevertheless, knowledge of mosquito embryogenesis, a pre-requisite to the establishment of transgenic individuals, has been presently neglected. We have her...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(98)00154-1
更新日期：1999-08-01 00:00:00
abstract：:The sand fly Lutzomyia longipalpis is the vector of Leishmania infantum, the etiological agent of American visceral leishmaniasis. Despite its importance, until now the internal anatomy of the immature forms has never been described and little is known about their digestive processes. In nature, sand fly larvae feed o...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2007.06.005
更新日期：2007-11-01 00:00:00
abstract：:In abdominal muscles 202 and 203 of the cricket, Gryllus bimaculatus, large and small excitatory junctional potentials (l- and s-EJPs) with similar durations can be recorded from the same muscle fibers. At the normal extracellular calcium ion concentration ([Ca(2+)](o)) of 5mM, the amplitudes of l-EJPs in both muscles...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(96)00087-x
更新日期：1997-02-21 00:00:00
abstract：:Lepidopteran males produce eupyrene (nucleate) and apyrene (anucleate) spermatozoa, but in the female only eupyrene spermatozoa leave the spermatheca and fertilize the eggs. Both kinds of spermatozoa lack intrinsic motility in the male genital duct. They become motile in the spermatophore, in a process involving prote...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(00)00109-8
更新日期：2001-03-01 00:00:00
abstract：:Juvenile hormone (JH) produced by the corpus allatum (CA) stimulates vitellogenesis and reduces the synthesis of hexamerin proteins in adult females of Pyrrhocoris apterus. At present it is unknown whether the signaling pathway involving the JH receptor gene Methoprene tolerant (Met) and its binding partner Taiman (Ta...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2016.08.009
更新日期：2016-01-01 00:00:00
abstract：:Females of Blattella germanica feed compulsively on a nuptial secretion from the male's eighth tergal gland (TG-8) during courtship. Using TG-8 extract and its essential ingredients, maltotriose (MT) and 1,2-dioleoylphosphatidylcholine (PC), we investigated the perception of the secretion by gustatory sensilla in both...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2009.04.014
更新日期：2009-08-01 00:00:00
abstract：:By single sensillum tip recording technique, in addition to the salt and pH cells found in antennal taste sensilla of some ground beetles earlier, the third chemosensory cell of four innervating these large sensilla was electrophysiologically identified as a sugar cell in the ground beetle Pterostichus aethiops. This ...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2006.12.012
更新日期：2007-04-01 00:00:00
abstract：:Wings and legs of the gregarious desert locust, Schistocerca gregaria have been shown to be release sites of phenylacetonitrile (PAN), the major adult male-produced pheromone. However, there is limited information on the distribution of PAN within the locust. Here we show, using gas chromatography-mass spectrometry (G...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2012.03.012
更新日期：2012-08-01 00:00:00
abstract：:The two-spotted spider mite, Tetranychus urticae was exposed to UV-C (250 nm), UV-B (300 nm), and UV-A (350 nm). In non-diapausing females, the median effective doses for 50% mortality plus escape incidence (ED(50)) were 21 (UV-C) and 104 kJm(-2) (UV-B); those for 50% oviposition rate in continuous darkness-treated mi...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2009.04.005
更新日期：2009-07-01 00:00:00
abstract：:Pheromonal control by the honey bee queen is achieved through the use of secretions from diverse glandular sources, but the use of pheromones from a variety of glandular sources by reproductively dominant workers, has not previously been explored. Using the social parasite, Apis mellifera capensis clonal worker we stu...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2017.09.001
更新日期：2017-10-01 00:00:00
abstract：:Males transfer sperm, proteins and other molecules to females during mating. In Drosophila melanogaster, these molecules contribute to the induction of egg maturation, ovulation, oviposition, sperm storage and changes in female receptivity. This suite of physiological and behavioral changes is referred to as the femal...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2017.04.004
更新日期：2017-05-01 00:00:00
abstract：:Endoparasitoid insects have evolved mechanisms to counteract host immune defences. At oviposition, the endoparasitoid Venturia canescens injects virus-like particles (VLPs) together with the egg that interfere with the immune system of the host. These prevent encapsulation of the parasitoid egg. It has been shown that...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2003.09.001
更新日期：2003-12-01 00:00:00
abstract：:Insect guts harbor diverse microbial assemblages that can be influenced by multiple factors, including gut physiology and interactions by the host with its environment. The Asian longhorned beetle (A. glabripennis; Cerambycidae: Lamiinae) is an invasive tree-killing insect that harbors a diverse consortium of fungal a...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2017.08.003
更新日期：2017-10-01 00:00:00
abstract：:Octopamine plays a major role in insect motor control and is released from dorsal unpaired median (DUM) neurones, a group of cells located on the dorsal midline of each ganglion. We were interested whether and how these neurones are activated during walking and chose the semi-intact walking preparation of stick insect...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2007.08.010
更新日期：2008-01-01 00:00:00
abstract：:Mating and immunity are two major components of fitness and links between them have been demonstrated in a number of recent investigations. In Drosophila melanogaster, a seminal fluid protein, sex-peptide (SP), up-regulates a number of antimicrobial peptide (AMP) genes in females after mating but the resulting effect ...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2007.10.010
更新日期：2008-02-01 00:00:00
abstract：:The ability of organisms to adapt to fluctuating food conditions is essential for their survival and reproduction. Accumulating energy reserves, such as lipids, in anticipation of harsh conditions, will reduce negative effects of a low food supply. For Hymenoptera and Diptera, several parasitoid species lack adult lip...
journal_title：Journal of insect physiology
pub_type： 杂志文章,评审
doi：10.1016/j.jinsphys.2008.07.014
更新日期：2008-09-01 00:00:00
abstract：:Susceptibility to the fungus Metarhizium robertsii and changes in host defences were evaluated in different stages of the intermoult period (4-6 h, 34-36 h and 84-86 h post moult in IV larval instars) of the Colorado potato beetle. A significant thickening of the cuticle during larval growth was accompanied by decreas...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2019.05.003
更新日期：2019-07-01 00:00:00
abstract：:To gain a better understanding of the molecular mechanisms regulating pupal diapause of the onion maggot Delia antiqua, PCR-based suppressive subtractive hybridization was performed to identify genes involved in summer and/or winter diapause. A total of 209 unique sequences were obtained including 89 in forward librar...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2012.08.010
更新日期：2012-11-01 00:00:00
abstract：:Electroantennogram (EAG) responses to the aphid sex pheromone components, (1R,4aS,7S,7aR)-nepetalactol and (4aS,7S,7aR)-nepetalactone, and a plant volatile, (E)-2-hexenal, were investigated at three different positions (5/6th, 4/5th and 3/4th inter-segmental regions) along the antennae of four different morphs in two ...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(02)00082-3
更新日期：2002-05-01 00:00:00
abstract：:Bactrocera dorsalis is a destructive fruit-eating pest that causes severe economic damage to the fruit and vegetable industry. Methyl eugenol (ME) has been widely used as an effective sexual attractant for male fruit flies through olfactory perception. However, the molecular mechanism underlying the olfactory percepti...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2012.05.011
更新日期：2012-08-01 00:00:00
abstract：:In some insect species, certain substances in the seminal fluid of males induce egg production and laying in females. We determined the effects of male-derived substances on female oviposition behaviour in two Callosobruchus species, C. chinensis and C. maculatus. Aqueous extracts of the accessory gland; testis; and s...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2010.07.008
更新日期：2010-12-01 00:00:00
abstract：:We studied behavioral responses of the cricket, Gryllus bimaculatus, to mechanical stimulation of the hindwing tip using three different kinds of stimuli: touching, bending and pinching. The most characteristic was a sequence of initial jump-like movements and subsequent running steps, that is referred to as escape be...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(00)00058-5
更新日期：2000-10-01 00:00:00
abstract：:Female grasshoppers of acoustically communicating species assume series of reproductive states that are associated with particular behaviours. Studies on laboratory populations of Chorthippus biguttulus (L.) revealed that females of this species lack the period of 'passive copulatory readiness', increase their attract...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2010.01.006
更新日期：2010-07-01 00:00:00
abstract：:The tropical fig borer, Batocera rufomaculata De Geer, is a large beetle that is a pest on a number of fruit trees, including fig and mango. Adults feed on the leaves and twigs and females lay their eggs under the bark of the tree. The larvae bore into the tree trunk, causing substantial damage that may lead to the co...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2017.02.008
更新日期：2017-04-01 00:00:00
abstract：:Queens of the primitively eusocial wasp Ropalidia marginata appear to maintain reproductive monopoly through pheromone rather than through physical aggression. Upon queen removal, one of the workers (potential queen, PQ) becomes extremely aggressive but drops her aggression immediately upon returning the queen. If the...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2010.11.014
更新日期：2011-02-01 00:00:00
abstract：:Insects lack the ability to synthesize sterols de novo so they acquire this essential nutrient from their food. Cholesterol is the dominant sterol found in most insects, but in plant vegetative tissue it makes up only a small fraction of the total sterol profile. Instead, plants mostly contain phytosterols; plant-feed...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2014.06.004
更新日期：2014-08-01 00:00:00
