Abstract：
:Apolipophorin III (apoLp-III) was isolated from the haemolymph of last instar larvae of Galleria mellonella. The ultraviolet (u.v.) spectrum and the N-terminal amino acid sequence reveal high similarities with the apoLp-III from Manduca sexta. The protein is heat-stable. The molecular mass of apoLp-III was determined to be 18 077 Da using mass spectrometry. The heat treatment (90 degrees C, 30 min) resulted in a pI shift from 6.6 for the non-heated to 6.1 for the heat-treated apoLp-III without change in the molecular mass, indicating that a conformational change might have been caused by the heat treatment, rather than covalent alterations. Intrahaemocoelic injection of pure apoLp-III into last instar G. mellonella larvae is followed by a dose-dependent increase of antibacterial activity in cell-free haemolymph of treated larvae 24 h after injection. Furthermore, pure apoLp-III enhances the phagocytic activity of isolated haemocytes in vitro. The newly discovered role of apoLp-III in inducing immune-related functions in insects is discussed in regard to the known features of this molecule in lipid metabolism. Arylphorin, another heat-stable protein in G. mellonella haemolymph, was likewise isolated in this study. The protein was identified by N-terminal protein sequencing, the sequence obtained exactly matches the known sequence data for this protein. Copyright 1997 Elsevier Science Ltd. All rights reserved
journal_name
J Insect Physioljournal_title
Journal of insect physiologyauthors
GOTZ P,WEISE C,KOPACEK P,LOSEN S,WIESNER Adoi
10.1016/s0022-1910(96)00113-8keywords:
subject
Has Abstractpub_date
1997-04-01 00:00:00pages
383-391issue
4eissn
0022-1910issn
1879-1611pii
S0022-1910(96)00113-8journal_volume
43pub_type
杂志文章abstract：:The corpora cardiaca (CC) of the Italian race (including also the africanised variety) of the honeybee (Apis mellifera ligustica) contain approximately 3 pmol of a hypertrehalosaemic peptide. This peptide is identical in structure to the adipokinetic hormone (AKH) found in Manduca sexta, Mas-AKH. The CC of the dark Eu...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(98)00158-9
更新日期：1999-07-01 00:00:00
abstract：:Enzyme-induced hemolysis has been shown to occur in the midgut of ticks; however, little is known about the molecular basis for hemolytic activity. We report here the molecular and reverse genetic characterization of a hemolytic midgut serine proteinase, HlSP, recently identified from the ixodid tick Haemaphysalis lon...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2006.12.001
更新日期：2007-02-01 00:00:00
abstract：:The adrenergic agonists octopamine, tyramine and clonidine inhibited the normal pheromonotropic action due to PBAN (pheromone biosynthesis activating neuropeptide) in incubations of intersegmental tissues that are situated between the 8th and 9th abdominal segments of the moth ovipositor tip. This inhibition was rever...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(96)00088-1
更新日期：1997-03-01 00:00:00
abstract：:A population of the fruit fly Drosophila melanogaster was raised in periodic light/dark (LD) cycles of 12:12 h for about 35 generations. Eclosion, locomotor activity, and oviposition were found to be rhythmic in these flies, when assayed in constant laboratory conditions where the light intensity, temperature, humidit...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(01)00107-x
更新日期：2001-09-01 00:00:00
abstract：:Air flow rates may confound the investigation and classification of insect gas exchange patterns. Here we report the effects of flow rates (50, 100, 200, 400 ml min(-1)) on gas exchange patterns in wild-caught Glossina morsitans morsitans from Zambia. At rest, G. m. morsitans generally showed continuous or cyclic gas ...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2009.02.005
更新日期：2010-05-01 00:00:00
abstract：:Plant-derived trypsin inhibitors have been shown to have potent anti-insect effects and are a promising alternative for the biological control of pests. In this work, we tested the anti-insect activity of Adenanthera pavonina trypsin inhibitor (ApTI) against Diatraea saccharalis larvae, a major insect pest in sugarcan...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2013.11.012
更新日期：2014-02-01 00:00:00
abstract：:Aedes aegypti mosquitoes use pharate 1st instar quiescence to cope with fluctuations in water availability hosting a fully developed 1st instar larvae within the chorion. The duration of this quiescence has been shown to affect larval fitness. This study sought to determine if an extended egg quiescence can elicit a p...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2012.12.007
更新日期：2013-03-01 00:00:00
abstract：:Ultraviolet (UV) radiation produces reactive oxygen species (ROS) in mammals, where melatonin plays the role of a ROS scavenger. The melatonin synthetic enzyme arylalkylamine N-acetyltransferase (NAT) is a significant element in a possible ROS removal system. Changes in NAT activity and melatonin content were determin...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2008.06.005
更新日期：2008-07-01 00:00:00
abstract：:Juvenile hormone content of Sesamia nonagrioides larvae reared under different environmental conditions that induce diapause was studied using a bioassay on newly-emerged Tribolium confusum pupae. Two analytical methods were also used to measure JHIII content. Extracts from larvae that developed under different condit...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(98)00019-5
更新日期：1998-05-01 00:00:00
abstract：:Although scientists know that overheating kills many organisms, they do not agree on the mechanism. According to one theory, referred to as oxygen- and capacity-limitation of thermal tolerance, overheating occurs when a warming organism's demand for oxygen exceeds its supply, reducing the organism's supply of ATP. Thi...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2020.104157
更新日期：2020-01-01 00:00:00
abstract：:The pheromone-sensitive receptor cells of male moth antennae are capable of detecting the rapid changes in stimulus intensity encountered in natural pheromone odour plumes. We investigated temporal response characteristics of the two receptor cell types of the sensillum trichodeum of female Bombyx mori, which are most...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(02)00109-9
更新日期：2002-08-01 00:00:00
abstract：:Certain male fruit flies from the genera Bactrocera and Zeugodacus (Diptera: Tephritidae) actively forage for floral semiochemicals produced by some endemic Bulbophyllum orchids found in tropical and subtropical forests. These floral semiochemicals are largely classified as either phenylbutanoids (e.g., raspberry keto...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2019.103949
更新日期：2019-01-01 00:00:00
abstract：:Brush border membrane vesicles (BBMVs) from Whole larvae of Aedes aegypti (AeBBMVWs) contain an H(+) V-ATPase (V), a Na(+)/H(+) antiporter, NHA1 (A) and a Na(+)-coupled, nutrient amino acid transporter, NAT8 (N), VAN for short. All V-ATPase subunits are present in the Ae. aegypti genome and in the vesicles. AgNAT8 was...
journal_title：Journal of insect physiology
pub_type： 杂志文章,评审
doi：10.1016/j.jinsphys.2010.04.017
更新日期：2010-10-01 00:00:00
abstract：:To develop inside their insect hosts, endoparasitoid wasps must either evade or overcome the host's immune system. Several ichneumonid and braconid wasps inject polydnaviruses that display well-studied immune suppressive effects. However, little is known about the strategies of immunoevasion used by other parasitoid f...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(03)00054-4
更新日期：2003-05-01 00:00:00
abstract：:To understand the inhibitory effects of high temperature on insect growth at the cellular level, we investigated the influence of high temperature on the proliferation and division of larval hemocytes in the silkworm, Bombyx mori. Although the total number of hemocytes in the larval body increased enormously over time...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2007.11.004
更新日期：2008-02-01 00:00:00
abstract：:Predicting how rapid climate change will affect terrestrial biota depends on a thorough understanding of an organism's biology and evolutionary history. Organisms at their range boundaries are particularly sensitive to climate change. As predominantly terrestrial poikilotherms, insects are often geographically limited...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2018.03.005
更新日期：2018-01-01 00:00:00
abstract：:Metabolic respiration by groups of resting Myrmica ruginodis and M. scabrinodis worker ants from five sites representing a range of latitudes, have been compared by measuring rates of CO(2) production-standardised by fat-free weight-at 5 and 25 degrees C. M. ruginodis which lives in cooler habitats than M. scabrinodis...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(98)00162-0
更新日期：1999-06-01 00:00:00
abstract：:To determine whether ecdysteroids are associated with reproductive division of labor in Bombus terrestris, we measured their levels in hemolymph and ovaries of queens and workers. Queens heading colonies had large active ovaries with high ecdysteroid content, whereas virgin gynes and mated queens before and after diap...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2005.03.009
更新日期：2005-07-01 00:00:00
abstract：:Juvenile hormones (JH) I, II, and III were monitored in hemolymph of virgin and mated females of various ages in Heliothis virescens. JH I was the predominant homologue followed by JH II, but JH II was present at a higher level in young virgin females. JH III was detectable only at a low level. In virgin females, hemo...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(97)00117-0
更新日期：1998-11-01 00:00:00
abstract：:Susceptibility to the fungus Metarhizium robertsii and changes in host defences were evaluated in different stages of the intermoult period (4-6 h, 34-36 h and 84-86 h post moult in IV larval instars) of the Colorado potato beetle. A significant thickening of the cuticle during larval growth was accompanied by decreas...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2019.05.003
更新日期：2019-07-01 00:00:00
abstract：:Bruchid beetles infest various seeds. The seed coat is the first protective barrier against bruchid infestation. Although non-host seed coats often impair the oviposition, eclosion and survival of the bruchid Callosobruchus maculatus larvae, morphological and biochemical aspects of this phenomenon remain unclear. Here...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2013.10.004
更新日期：2014-01-01 00:00:00
abstract：:Pepck is a metabolic enzyme that participates in gluconeogenesis through the conversion of oxaloacetate into phosphoenol pyruvate. Numerous transcriptomic studies have identified Pepck as a potential key player during diapause and various stresses responses. Here, we describe expression patterns of both cytosolic and ...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2018.10.008
更新日期：2018-01-01 00:00:00
abstract：:The quantity and composition of the six major mandibular gland components of young queenless workers of the Cape honeybee (Apis mellifera capensis) were determined. The total amount of the six components increased with age. The relative quantities in the mandibular gland secretion of queenless caged workers were found...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(00)00167-0
更新日期：2001-07-01 00:00:00
abstract：:Many animals have been shown to select among nutritionally complementary foods to reach a specific balance of nutrients that optimizes key life history traits. Nutritional ecology theory, however, predicts that an animal with a diet that is very stable in its composition, and with nutritional requirements that do not ...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2019.103983
更新日期：2020-01-01 00:00:00
abstract：:Mating and immunity are two major components of fitness and links between them have been demonstrated in a number of recent investigations. In Drosophila melanogaster, a seminal fluid protein, sex-peptide (SP), up-regulates a number of antimicrobial peptide (AMP) genes in females after mating but the resulting effect ...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2007.10.010
更新日期：2008-02-01 00:00:00
abstract：:We investigated if Brindley's and metasternal glands are involved in the sexual behavior of Triatoma infestans. In laboratory assays, we analyzed the effect of selective occlusion of Brindley's and metasternal glands of the female (separately and together) on the behavior of males. Control assays without occlusion of ...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2007.03.014
更新日期：2007-07-01 00:00:00
abstract：:This study re-examines the effects of environmental factors including substrate color, humidity, food quality, light intensity and temperature on the green-brown polyphenism, black patterning and background body color of solitarious (isolated-reared) nymphs of Schistocerca gregaria. All individuals reared in yellow-gr...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2011.10.002
更新日期：2012-01-01 00:00:00
abstract：:The last-instar larvae of a drosophilid fly, Chymomyza costata enter diapause in response to the dark-phases longer than 9 h (Yoshida, T., Kimura, M.T., 1995. The photoperiodic clock in Chymomyza costata. Journal of Insect Physiology 41, 217-222). In order to switch the developmental programming of the sensitive larva...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(99)00195-x
更新日期：2000-06-01 00:00:00
abstract：:Sublethal concentrations of the bisacylhydrazine moulting hormone agonists, RH-5849, and tebufenozide (RH-5992) were fed to sixth (final) instar larvae of Spodoptera litura. Both RH-5849 and tebufenozide adversely affected the mating success of S. litura when the surviving treated males were crossed with normal female...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2004.03.007
更新日期：2004-06-01 00:00:00
abstract：:Tetranychus urticae (Acari: Tetranychidae) possesses a sac-like ovary with characteristic oocytes that protrude from the ovarian surface. In nondiapause females, transparent oocytes became opaque with yolk deposition between days 0 and 1 in the adult stage at 20 degrees C. In diapause females, however, ovarian develop...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2008.10.007
更新日期：2009-01-01 00:00:00
