Abstract：
:Pepck is a metabolic enzyme that participates in gluconeogenesis through the conversion of oxaloacetate into phosphoenol pyruvate. Numerous transcriptomic studies have identified Pepck as a potential key player during diapause and various stresses responses. Here, we describe expression patterns of both cytosolic and mitochondrial isoforms of Pepck throughout development, during diapause, and in response to starvation and cold shock in the flesh fly, Sarcophaga bullata. We cloned full-length transcripts for both Pepck isoforms and observed that expression of both genes varied throughout development. Diapausing pupae have the highest relative expression of both isoforms, suggesting participation in the anticipatory production of sugars and sugar alcohols that occurs during this overwintering stage. In response to acute stress, the cytosolic isoform was upregulated whereas the mitochondrial variant remained unchanged. Cytosolic Pepck was strongly upregulated after 2 h recovery from cold shock and returned to baseline levels within 8 h. In response to 24 h of starvation, the cytosolic isoform was similarly upregulated and returned to control levels after 24 h of recovery. Acute stress is known to incur a metabolic cost, and Pepck could be a key player in this response. Although it remains unclear why there is such a dramatic divergence in the expression of the two isoforms, the distinction suggests specific roles for the two isoforms that depend on the developmental status of the fly and the stress conditions to which it is exposed.
journal_name
J Insect Physioljournal_title
Journal of insect physiologyauthors
Spacht DE,Teets NM,Denlinger DLdoi
10.1016/j.jinsphys.2018.10.008subject
Has Abstractpub_date
2018-01-01 00:00:00pages
41-46eissn
0022-1910issn
1879-1611pii
S0022-1910(18)30282-8journal_volume
111pub_type
杂志文章abstract：:Experiments were conducted to determine the performance and host preference of Ascia monuste using kale (Brassica oleraceae, var. Acephala) and mustard (B. juncea). These plants differ significantly in water and nitrogen content, with mustard having larger amounts of water and kale larger amounts of nitrogen. The perf...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(98)00094-8
更新日期：1999-01-01 00:00:00
abstract：:Chemosensory proteins (CSPs) are a family of small soluble proteins that, in addition to the odorant-binding proteins (OBPs), are involved in chemical communication. To understand the physiological function of the 16 known CSPs in the silkworm Bombyx mori, we investigated the expression patterns in different tissues a...
journal_title：Journal of insect physiology
pub_type： 杂志文章
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更新日期：2013-07-01 00:00:00
abstract：:Copula duration and sperm storage patterns can directly or indirectly affect fitness of male and female insects. Although both sexes have an interest in the outcome, research has tended to focus on males. To investigate female influences, we compared copula duration and sperm storage of Queensland fruit fly females th...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2010.07.005
更新日期：2010-12-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2006.07.004
更新日期：2006-10-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章,评审
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更新日期：2019-04-01 00:00:00
abstract：:The environmental physiology of three speciesof Collembola: Cryptopygus cisantarcticus, Isotoma klovstadi (Isotomidae) and Friesea grisea (Neanuridae) was investigated from November 2002 to February 2003 at Cape Hallett, North Victoria Land, Antarctica. All three species were freeze avoiding, and while supercooling po...
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pub_type： 杂志文章
doi：10.1016/j.jinsphys.2005.09.002
更新日期：2006-01-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(03)00118-5
更新日期：2003-08-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2013.07.004
更新日期：2013-09-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2019.103948
更新日期：2019-01-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2008.02.003
更新日期：2008-05-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2015.12.002
更新日期：2016-02-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2018.01.006
更新日期：2018-01-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2016.07.004
更新日期：2016-08-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2010.01.006
更新日期：2010-07-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2007.11.011
更新日期：2008-02-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2009.05.020
更新日期：2010-05-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2006.10.009
更新日期：2007-07-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(03)00047-7
更新日期：2003-06-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2005.03.009
更新日期：2005-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.jinsphys.2013.11.010
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2005.01.009
更新日期：2005-06-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2015.03.008
更新日期：2015-05-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
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更新日期：1998-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.jinsphys.2019.103931
更新日期：2019-10-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2018.06.002
更新日期：2018-01-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2014.05.005
更新日期：2014-06-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2014.02.002
更新日期：2014-04-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2020.104100
更新日期：2020-10-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(01)00165-2
更新日期：2002-02-01 00:00:00
abstract：:Nine neuropeptides isolated from Drosophila melanogaster and five neuropeptides, previously isolated from the CNS of Limulus with antisera to FMRFamide-related peptides, were tested for their effects on the myogenic heart of Drosophila melanogaster. Of the native peptides, TDVDHVFLRF-NH(2) (Dromyosuppressin), DPKQDFMR...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(00)00043-3
更新日期：2000-08-01 00:00:00
