Abstract:
:Bactrocera dorsalis is a destructive fruit-eating pest that causes severe economic damage to the fruit and vegetable industry. Methyl eugenol (ME) has been widely used as an effective sexual attractant for male fruit flies through olfactory perception. However, the molecular mechanism underlying the olfactory perception of ME remains unknown. Here, we report the characterization and functional analysis of a newly discovered cDNA that encodes a Drosophila melanogaster odorant receptor co-receptor Orco ortholog in B. dorsalis. qRT-PCR analysis revealed that it was abundantly expressed in the antenna of adult B. dorsalis. Notably, Orco was upregulated by ME in the antenna of male flies. Mature males of B. dorsalis showed significant taxis toward ME within 0.5h, and Orco was significantly upregulated in the attracted adults within the same period. Silencing Orco through the ingestion of dsRNA reduced the attractive effects of ME. These data suggest that Orco may play an essential role in ME attraction in the olfactory signal transduction pathway.
journal_name
J Insect Physioljournal_title
Journal of insect physiologyauthors
Zheng W,Zhu C,Peng T,Zhang Hdoi
10.1016/j.jinsphys.2012.05.011subject
Has Abstractpub_date
2012-08-01 00:00:00pages
1122-7issue
8eissn
0022-1910issn
1879-1611pii
S0022-1910(12)00126-6journal_volume
58pub_type
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