Molecular composition of the wall of insect olfactory sensilla-the chitin question.

Abstract:

:Identification of chitin in sensory hairs of olfactory sensilla of silkmoths was performed using two independent methods. Firstly, ultrathin sections were labelled with gold-conjugated wheat germ agglutinin and showed positive labelling in the cuticule of sensilla as well as in the antennal cuticle. Secondly, isolated sensory hairs and body scales were subjected to analytical pyrolysis in combination with gas chromatography and mass spectrometry. Chromatograms of both sensory hairs and scales, included several pyrolysis products, which unequivocally demonstrate the contribution of chitinous moieties to the chemical composition of both types of cuticle. This study supports the notion that even the very thin cuticle of olfactory sensilla is composed of both an epi- and a true exocuticle. The carbohydrate components of the latter cuticle most probably are responsible for the extremely high resilience and breaking limit of these delicate structures.

journal_name

J Insect Physiol

authors

Steinbrecht RA,Stankiewicz BA

doi

10.1016/s0022-1910(99)00066-9

keywords:

subject

Has Abstract

pub_date

1999-08-01 00:00:00

pages

785-790

issue

8

eissn

0022-1910

issn

1879-1611

pii

S0022191099000669

journal_volume

45

pub_type

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