Hemolytic activity is mediated by the endogenous lectin in the mosquito hemolymph serum.

Abstract:

:Although cytolysis of invading organisms is an innate form of immunity used by invertebrates, so far the underlying mechanism remains less explored. The pupal hemolymph of the mosquito Armigeres subalbatus induces an activity that causes hemolysis of human red blood cells (HRBC). This hemolytic activity was inhibited by sialic acid (N-acetylneuraminic acid) and serine protease inhibitors. We purified the sialic acid-specific lectin(s) from the pupal hemolymph using formaldehyde-fixed HRBC and determined the sequence of the amino-terminal 19 amino acid residues. A polyclonal antibody produced against this N-terminal peptide clearly inhibited the hemolytic activity of the hemolymph in vitro, thus suggesting that the hemolysis of HRBC is caused by the lectin present in the mosquito hemolymph. We suggest that mosquitoes possess a cytolysis system.

journal_name

J Insect Physiol

authors

Sasaki T,Hiraoka T,Kobayashi M

doi

10.1016/j.jinsphys.2010.02.007

subject

Has Abstract

pub_date

2010-09-01 00:00:00

pages

1032-9

issue

9

eissn

0022-1910

issn

1879-1611

pii

S0022-1910(10)00052-1

journal_volume

56

pub_type

杂志文章
  • Effect of helicokinins and ACE inhibitors on water balance and development of Heliothis virescens larvae.

    abstract::The diuretic activity of the helicokinins I (YFSPWG-amide), II (VRFSPWG-amide) and III (KVKFSAWG-amide) was tested on Heliothis virescens larvae. All three kinins increased fluid secretion in isolated Malpighian tubules in a dose-dependent manner. Injections into the haemolymph caused a significant reduction in weight...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/s0022-1910(00)00065-2

    authors: Seinsche A,Dyker H,Lösel P,Backhaus D,Scherkenbeck J

    更新日期:2000-11-01 00:00:00

  • JH modulates a cellular immunity of Tribolium castaneum in a Met-independent manner.

    abstract::Juvenile hormone (JH) regulates diverse physiological processes in insects during entire developmental stages. Especially, the identification of Methoprene-tolerant (Met), a JH nuclear receptor, allows us to better understand molecular actions of JH to control gene expressions related with metamorphosis. However, seve...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2014.02.008

    authors: Hepat R,Kim Y

    更新日期:2014-04-01 00:00:00

  • Concentration and distribution of diaphorin, and expression of diaphorin synthesis genes during Asian citrus psyllid development.

    abstract::The Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Sternorrhyncha: Psylloidea: Liviidae) is an important pest of citrus species worldwide because it transmits Candidatus Liberibacter spp. (Alphaproteobacteria); the causative agents of an incurable citrus disease known as huanglongbing or greening disease. ...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2019.103931

    authors: Nakabachi A,Fujikami M

    更新日期:2019-10-01 00:00:00

  • Transcriptional response to dietary restriction in Drosophila melanogaster.

    abstract::Dietary restriction (DR) extends lifespan in a wide variety of organisms. Although several genes and pathways associated with this longevity response have been identified, the specific mechanism through which DR extends lifespan is not fully understood. We have recently developed a novel methodology to screen for tran...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2014.05.002

    authors: Ding F,Gil MP,Franklin M,Ferreira J,Tatar M,Helfand SL,Neretti N

    更新日期:2014-10-01 00:00:00

  • Involvement of 4E-BP phosphorylation in embryonic development of the silkworm, Bombyx mori.

    abstract::Phosphorylation of the translational repressor 4E-binding protein (4E-BP) plays a critical role in regulating the overall translation levels in cells. In the present study, we investigated 4E-BP phosphorylation of Bombyx mori eggs by an immunoblot analysis of a conserved phospho-specific antibody to 4E-BP and demonstr...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2011.04.014

    authors: Gu SH,Young SC,Tsai WH,Lin JL,Lin PL

    更新日期:2011-07-01 00:00:00

  • Glandular sources of pheromones used to control host workers (Apis mellifera scutellata) by socially parasitic workers of Apis mellifera capensis.

    abstract::Pheromonal control by the honey bee queen is achieved through the use of secretions from diverse glandular sources, but the use of pheromones from a variety of glandular sources by reproductively dominant workers, has not previously been explored. Using the social parasite, Apis mellifera capensis clonal worker we stu...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2017.09.001

    authors: Okosun OO,Pirk CWW,Crewe RM,Yusuf AA

    更新日期:2017-10-01 00:00:00

  • Differential expression, phosphorylation of COX subunit 1 and COX activity during diapause phase in the cotton bollworm, Helicoverpa armigera.

    abstract::Helicoverpa armigera (Lepidoptera, Noctuidae) is an important agricultural pest with a pupal diapause. Cytochrome c oxidase (COX) is a key speed-limited enzyme of oxidative phosphorylation in mitochondria for ATP production. A differentially expressed cDNA fragment encoding COX subunit 1 (cox1) was cloned by different...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2010.08.023

    authors: Yang J,Zhu J,Xu WH

    更新日期:2010-12-01 00:00:00

  • Laccase-type phenoloxidase in salivary glands and watery saliva of the green rice leafhopper, Nephotettix cincticeps.

    abstract::The activity and composition of leafhopper saliva are important in interactions with the host rice plant, and it may play a physiological role in detoxifying toxic plant substances or ingesting sap. We have characterized diphenoloxidase in the salivary glands of Nephotettix cincticeps, its activity as a laccase, and i...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2005.08.010

    authors: Hattori M,Konishi H,Tamura Y,Konno K,Sogawa K

    更新日期:2005-12-01 00:00:00

  • Overwintering adaptations in the lettuce root aphid Pemphigus bursarius (L.).

    abstract::Pemphigus bursarius (L.) is a host alternating root-feeding aphid with a proportion of the population overwintering as asexual hiemalis in the soil. These hiemalis must be sufficiently cold tolerant to survive at the temperatures they would experience in winter, and also be able to overcome a period of prolonged starv...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/s0022-1910(99)00188-2

    authors: Phillips SW,Bale JS,Tatchell GM

    更新日期:2000-03-01 00:00:00

  • Seminal fluids mediate sexual inhibition and short copula duration in mated female Queensland fruit flies.

    abstract::Molecules in male seminal fluid transferred to female insects during mating can have potent effects on their subsequent sexual and reproductive behaviour. Like many other tephritids, female Queensland fruit flies (Bactrocera tryoni) typically have diminished sexual receptivity after their first mating. Also, copulatio...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2006.10.009

    authors: Radhakrishnan P,Taylor PW

    更新日期:2007-07-01 00:00:00

  • Segment-specific Ca(2+) transport by isolated Malpighian tubules of Drosophila melanogaster: A comparison of larval and adult stages.

    abstract::Haemolymph calcium homeostasis in insects is achieved through the regulation of calcium excretion by Malpighian tubules in two ways: (1) sequestration of calcium within biomineralized granules and (2) secretion of calcium in soluble form within the primary urine. Using the scanning ion-selective electrode technique (S...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2016.01.005

    authors: Browne A,O'Donnell MJ

    更新日期:2016-04-01 00:00:00

  • Evolving ideas about genetics underlying insect virulence to plant resistance in rice-brown planthopper interactions.

    abstract::Many plant-parasite interactions that include major plant resistance genes have subsequently been shown to exhibit features of gene-for-gene interactions between plant Resistance genes and parasite Avirulence genes. The brown planthopper (BPH) Nilaparvata lugens is an important pest of rice (Oryza sativa). Historicall...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章,评审

    doi:10.1016/j.jinsphys.2015.12.001

    authors: Kobayashi T

    更新日期:2016-01-01 00:00:00

  • Ultrastructural Effects of a Non-Steroidal Ecdysone Agonist, RH-5992, on the Sixth Instar Larva of the Spruce Budworm, Choristoneura fumiferana.

    abstract::Force feeding of RH-5992 (Tebufenozide), a non-steroidal ecdysone agonist to newly moulted sixth instar larvae of the spruce budworm, Choristoneura fumiferana, (Lepidoptera: Tortricidae) initiates a precocious, incomplete moult. Within 6h post treatment (pt) the larva stops feeding and remains quiescent. Around 12hpt,...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/s0022-1910(96)00062-5

    authors: Palli SR,Brownwright AJ,Davis CN,Tomkins WL,MacDonald A,Retnakaran A

    更新日期:1997-02-19 00:00:00

  • Environmental causes and transgenerational consequences of ecdysteroid hormone provisioning in Acheta domesticus.

    abstract::An animal's phenotype may be shaped by its genes, but also reflects its own environment and often that of its parents. Nongenetic parental effects are often mediated by steroid hormones, and operate between parents and offspring through mechanisms that are well described in vertebrate and model systems. However, less ...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2018.06.003

    authors: Crocker KC,Hunter MD

    更新日期:2018-01-01 00:00:00

  • Long-term effect of systemic RNA interference on circadian clock genes in hemimetabolous insects.

    abstract::RNA interference (RNAi) strategy, which enables gene-specific knock-down of transcripts, has been spread across a wide area of insect studies for investigating gene function without regard to model and non-model insects. This technique is of particular benefit to promote molecular studies on non-model insects. However...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2013.02.009

    authors: Uryu O,Kamae Y,Tomioka K,Yoshii T

    更新日期:2013-04-01 00:00:00

  • Brain tyramine and reproductive states of workers in honeybees.

    abstract::To explore the role of tyramine in the transformation of reproductive states of honeybee workers, brain levels of tyramine and N-acetyltyramine were measured in both normal and queenless workers. Queenless workers had higher tyramine levels and lower N-acetyltyramine levels than normal workers did. Intermediate reprod...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/s0022-1910(02)00200-7

    authors: Sasaki K,Nagao T

    更新日期:2002-12-01 00:00:00

  • Nutritional value and taste play different roles in learning and memory in the honey bee (Apis mellifera).

    abstract::Honey bees will learn to respond to an odor when their antennae are stimulated with sucrose, even if they are not fed during the conditioning phase. However, if they are not fed, the memory of this association is significantly reduced 24 h after conditioning. These results suggest that stimulation of proboscis with su...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2018.04.014

    authors: Mustard JA,Alvarez V,Barocio S,Mathews J,Stoker A,Malik K

    更新日期:2018-01-01 00:00:00

  • Short-term persistence precedes pathogenic infection: Infection kinetics of cricket paralysis virus in silkworm-derived Bm5 cells.

    abstract::Next generation sequencing has revealed the widespread occurrence of persistent virus infections in insects but little is known regarding to what extent persistent infections can affect cellular physiology and how they might contribute to the development of disease. In contrast to the pathogenic infections occurring i...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2019.03.004

    authors: Wang L,Cappelle K,Santos D,Vanden Broeck J,Smagghe G,Swevers L

    更新日期:2019-01-01 00:00:00

  • Dietary self-selection and discrimination threshold in wild Anastrepha obliqua females (Diptera, Tephritidae).

    abstract::Experiments were performed on adult wild Anastrepha obliqua females to (I) determine whether there is a critical period during the reproductive phase when the absence of protein in the diet impairs egg production; (II) determine whether the females are able to self-select an optimal diet; and (III) determine the discr...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/s0022-1910(01)00093-2

    authors: Cresoni-Pereira C,Zucoloto FS

    更新日期:2001-09-01 00:00:00

  • Identification of odorant binding proteins in Carpomya vesuviana and their binding affinity to the male-borne semiochemicals and host plant volatiles.

    abstract::Carpomya vesuviana (Costa; Diptera: Tephritidae) is an agricultural pest that causes serious damage to jujube fruits. However, the mechanism of olfaction, which is critical for host identification, is not well understood in this pest. In this study, we have identified for the first time five protein types involved in ...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2017.05.013

    authors: Li Y,Zhou P,Zhang J,Yang D,Li Z,Zhang X,Zhu S,Yu Y,Chen N

    更新日期:2017-07-01 00:00:00

  • Corazonin- and PDF-immunoreactivities in the cephalic ganglia of termites.

    abstract::Antisera against the pigment-dispersing factor (PDF) and corazonin (Crz) reacted with distinct sets of neurons in the cephalic ganglia of termites. The locations of immunoreactive cells were similar but their numbers differed among the eight species examined: PDF-ir occurred in 0-6 cells in each optic lobe and 1-2 pai...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2008.11.009

    authors: Závodská R,Wen CJ,Sehnal F,Hrdý I,Lee HJ,Sauman I

    更新日期:2009-05-01 00:00:00

  • Re-examination of the roles of environmental factors in the control of body-color polyphenism in solitarious nymphs of the desert locust Schistocerca gregaria with special reference to substrate color and humidity.

    abstract::This study re-examines the effects of environmental factors including substrate color, humidity, food quality, light intensity and temperature on the green-brown polyphenism, black patterning and background body color of solitarious (isolated-reared) nymphs of Schistocerca gregaria. All individuals reared in yellow-gr...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2011.10.002

    authors: Tanaka S,Harano K,Nishide Y

    更新日期:2012-01-01 00:00:00

  • Expression profiling and comparative analyses of seven midgut serine proteases from the yellow fever mosquito, Aedes aegypti.

    abstract::Aedes aegypti utilizes blood for energy production, egg maturation and replenishment of maternal reserves. The principle midgut enzymes responsible for bloodmeal digestion are endoproteolytic serine-type proteases within the S1.A subfamily. While there are hundreds of serine protease-like genes in the A. aegypti genom...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2010.01.003

    authors: Brackney DE,Isoe J,W C B 4th,Zamora J,Foy BD,Miesfeld RL,Olson KE

    更新日期:2010-07-01 00:00:00

  • Polymorphism of Colias croceus from the Azores caused by differential pterin expression in the wing scales.

    abstract::The pierid butterfly Colias croceus (Geoffroy in Fourcroy, 1785), established in the Azores archipelago, is polymorphic with six forms, C. croceus f. croceus ♂ and ♀, C. c. f. cremonae ♂ and ♀, C. c. f. helice ♀, and C. c. f. cremonaehelice ♀. We investigated the optical mechanisms underlying the wing colouration of t...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2020.104114

    authors: Hutsebaut J,Leertouwer HL,Stavenga DG

    更新日期:2020-01-01 00:00:00

  • Tri-trophic effects of transgenic insect-resistant tobacco expressing a protease inhibitor or a biotin-binding protein on adults of the predatory carabid beetle Ctenognathus novaezelandiae.

    abstract::Tri-trophic impacts on adult predatory carabid beetles, Ctenognathus novaezelandiae, of insect-resistant transgenic tobacco plants expressing a serine protease inhibitor, bovine spleen trypsin inhibitor (BSTI), or a biotin-binding protein, avidin, were investigated. Both proteins could potentially affect this beetle, ...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2007.12.002

    authors: Burgess EP,Philip BA,Christeller JT,Page NE,Marshall RK,Wohlers MW

    更新日期:2008-02-01 00:00:00

  • Population-associated heterogeneity of the digestive Cys protease complement in Colorado potato beetle, Leptinotarsa decemlineata.

    abstract::Herbivorous insects use complex protease complements to process plant proteins, useful to adjust their digestive functions to the plant diet and to elude the antidigestive effects of dietary protease inhibitors. We here assessed whether basic profiles and diet-related adjustments of the midgut protease complement may ...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2017.03.001

    authors: Rasoolizadeh A,Goulet MC,Guay JF,Cloutier C,Michaud D

    更新日期:2018-04-01 00:00:00

  • Endocrine changes associated with metamorphosis and diapause induction in the yellow-spotted longicorn beetle, Psacothea hilaris.

    abstract::At 25 degrees C and under a long-day photoperiod, all 5th instar Psacothea hilaris larvae pupate at the next molt. Under a short-day photoperiod, in contrast, they undergo one or two additional larval molts and enter diapause; the 7th instar larvae enter diapause without further molt. The changes in hemolymph juvenile...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2004.09.009

    authors: Munyiri FN,Ishikawa Y

    更新日期:2004-11-01 00:00:00

  • Juvenile hormone titers, ovarian status and epicuticular hydrocarbons in gynes and workers of the paper wasp Belonogaster longitarsus.

    abstract::The prevailing paradigm for social wasp endocrinology is that of juvenile hormone (JH) functioning pleiotropically in potential and actual queens, where it fuels dominance behaviors, stimulates ovarian growth and/or affects the production of status-linked cuticular compounds. In colonies with annual cycles (e.g., temp...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2016.11.014

    authors: Kelstrup HC,Hartfelder K,Esterhuizen N,Wossler TC

    更新日期:2017-04-01 00:00:00

  • Critical thermal limits, temperature tolerance and water balance of a sub-Antarctic caterpillar, Pringleophaga marioni (Lepidoptera: Tineidae).

    abstract::Thermal tolerance, supercooling point, water balance and osmoregulatory ability of Pringleophaga marioni Viette (Lepidoptera: Tineidae) are investigated in this study. Field-fresh larvae had a mean CT(Min) (cold stupor) of -0.6 degrees C and a mean CT(Max) (heat coma) of 38.7 degrees C. The mean supercooling point of ...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/s0022-1910(97)00001-2

    authors: Chown SL,Jaco Klok C

    更新日期:1997-07-01 00:00:00

  • Immunological mechanisms of synergy between fungus Metarhizium robertsii and bacteria Bacillus thuringiensis ssp. morrisoni on Colorado potato beetle larvae.

    abstract::The synergistic effect between the entomopathogenic fungus Metarhizium robertsii and a sublethal dose of the bacterium Bacillus thuringiensis ssp. morrisoni var. tenebrionis was studied in terms of immune defense reactions and detoxification system activity of the Colorado potato beetle, Leptinotarsa decemlineata, fou...

    journal_title:Journal of insect physiology

    pub_type: 杂志文章

    doi:10.1016/j.jinsphys.2016.10.004

    authors: Yaroslavtseva ON,Dubovskiy IM,Khodyrev VP,Duisembekov BA,Kryukov VY,Glupov VV

    更新日期:2017-01-01 00:00:00