Abstract：
:Fifth instar larvae and prepupae of Heliothis virescens (tobacco budworm) were injected with live Enterobacter cloacae and bled at different times after vaccination. Immune pupal hemolymph showed a 54 times increase in lysozyme activity when compared with normal larval hemolymph, and an 11 times increase of lysozyme activity when compared with immune larval hemolymph. Lysozyme activity of the normal pupal hemolymph increased as greatly as did lysozyme activity of the immune larval hemolymph after metamorphosis. The pupal immune response with regard to lysozyme was much greater than the larval immune response in H. virescens. Lysozyme was purified by heat treatment at 100 degrees C and a chromatography series that included reverse-phase HPLC. The molecular mass of H. virescens lysozyme was approximately 16 kDa by SDS-PAGE which is greater than other insect lysozymes and chicken lysozyme. Amino acid sequence of the N-terminus showed that H. virescens lysozyme is 82% homologous with lysozyme of Manduca sexta and Galleria mellonella. CNBr cleavage of H. virescens lysozyme produced 11 and 6 kDa peptide fragments indicating that one methionine was present, which was also supported by amino acid analysis. However, methionine was located at the carboxyl terminal side rather than the N-terminal side as judged by the N-terminal sequences of each peptide fragment. The residue 22 in most lepidopteran lysozymes is methionine, whereas H. virescens lysozyme had a leucine at residue 22. There was an amino acid deletion near the carboxyl terminal side of H. virescens lysozyme as also found in Trichoplusia ni.
journal_name
J Insect Physioljournal_title
Journal of insect physiologyauthors
Chung KT,Ourth DDdoi
10.1016/s0022-1910(99)00142-0keywords:
subject
Has Abstractpub_date
2000-04-01 00:00:00pages
563-572issue
4eissn
0022-1910issn
1879-1611pii
S0022-1910(99)00142-0journal_volume
46pub_type
杂志文章abstract：:Although the pupae and larvae of Bombyx mori possess especially large amounts of free d-serine, the physiological role of the amino acid in the silkworm is unknown. We investigated the effect of d-serine on spermatogenesis. A lowered d-serine level throughout larval development caused a delay in spermatogenesis and re...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2014.06.003
更新日期：2014-08-01 00:00:00
abstract：:Sugarcane is an important crop that has recently become subject to attacks from the weevil Sphenophorus levis, which is not efficiently controlled with chemical insecticides. This demands the development of new control devices for which digestive physiology data are needed. In the present study, ion-exchange chromatog...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2011.01.006
更新日期：2011-04-01 00:00:00
abstract：:Insect hemocytes play an important role in insects' defense against environmental stressors as they are entirely dependent on their innate immune system for pathogen defense. In recent years a dramatic decline of pollinators has been reported in many countries. The drivers of this declines appear to be associated with...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2018.05.002
更新日期：2018-07-01 00:00:00
abstract：:Body size and shape affect thermoregulatory properties of organisms, and in turn are believed to have shaped macroevolutionary patterns of morphological diversity across many taxa. However, it is less clear whether thermoregulation plays a role in shaping intraspecific morphological diversity such as sexual dimorphism...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2007.12.007
更新日期：2008-03-01 00:00:00
abstract：:Bruchid beetles infest various seeds. The seed coat is the first protective barrier against bruchid infestation. Although non-host seed coats often impair the oviposition, eclosion and survival of the bruchid Callosobruchus maculatus larvae, morphological and biochemical aspects of this phenomenon remain unclear. Here...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2013.10.004
更新日期：2014-01-01 00:00:00
abstract：:Haemocytes play crucial roles in immune responses and survival in insects. Specific cell markers have proven effective in clarifying the function and haematopoiesis of haemocytes. The silkworm Bombyx mori is a good model for studying insect haemocytes; however, little is known about haemocyte-specific markers or their...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2014.10.007
更新日期：2014-12-01 00:00:00
abstract：:Pheromonal control by the honey bee queen is achieved through the use of secretions from diverse glandular sources, but the use of pheromones from a variety of glandular sources by reproductively dominant workers, has not previously been explored. Using the social parasite, Apis mellifera capensis clonal worker we stu...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2017.09.001
更新日期：2017-10-01 00:00:00
abstract：:In Drosophila multiple circadian oscillators and behavioral rhythms are known to exist, yet most previous studies that attempted to understand circadian entrainment have focused on the activity/rest rhythm and to some extent the adult emergence rhythm. Egg laying behavior of Drosophila females also follows circadian r...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2011.11.012
更新日期：2012-02-01 00:00:00
abstract：:The immune system of Rhodnius prolixus comprehends the synthesis of different effectors that modulate the intestinal microbiota population and the life cycle of the parasite Trypanosoma cruzi inside the vector midgut. One of these immune responses is the production of reactive nitrogen species (RNS) derived by the act...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2020.104100
更新日期：2020-10-01 00:00:00
abstract：:To examine the function of silkworm Bombyx mori L. athrocytes (nephrocytes), we constructed cDNAs of larval peritracheal athrocytes that were anatomically isolated from surrounding tissues. Larval expression levels of genes encoding hemolymph proteins, such as arylphorin, the 30K proteins, and lysozyme, were lower in ...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2008.06.011
更新日期：2008-08-01 00:00:00
abstract：:Apis mellifera treated during different developmental phases with pyriproxyfen, a juvenile hormone analogue, show profound alterations in cuticular pigmentation and sclerotization. When the treatment is effected during the feeding phase of the fifth larval instar (LF5), the pupal development is blocked and pigmentatio...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(97)00113-3
更新日期：1998-05-01 00:00:00
abstract：:Amblyseius californicus was introduced into the UK in the early 1990s as a biocontrol agent against glasshouse red spider mite Tetranychus urticae. This study investigated the effects of temperature on the establishment potential of A. californicus in the UK in the light of recent reports of their successful overwinte...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(02)00087-2
更新日期：2002-06-01 00:00:00
abstract：:Nitric oxide (NO) is known as a gaseous messenger in the nervous system. It plays a role in synaptic plasticity, but also in development and regeneration of nervous systems. We have studied the function of NO and its signaling cascade via cyclic GMP in the locust embryo. Its developing nervous system is well suited fo...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2010.03.031
更新日期：2010-08-01 00:00:00
abstract：:A two-step HPLC purification procedure resulted in a factor from the stable fly that depolarizes the lumen-negative transepithelial voltage (V(t)) of the adult stable fly Malpighian tubule. When applied to tubules of the female mosquito, Aedes aegypti, this factor partially mimics the electrophysiological actions of t...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(97)00078-4
更新日期：1997-10-01 00:00:00
abstract：:In this study, we investigated activation of the extracellular signal-regulated kinase (ERK) by the prothoracicotropic hormone (PTTH) in prothoracic gland cells of the silkworm, Bombyx mori. The results showed that the PTTH stimulated ERK phosphorylation as this depends on time and dose and ecdysteroidogenic activity....
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2007.03.004
更新日期：2007-06-01 00:00:00
abstract：:Sperm cells are costly to produce; diet should therefore affect sperm number and/or viability. In non-social insects and vertebrates, there is compelling evidence that diet influences sperm production. Less is known about this relationship in eusocial hymenopterans (all ants and some bees and wasps), whose mating syst...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2017.05.012
更新日期：2017-07-01 00:00:00
abstract：:Animals often alter their food choices following a pathogen infection in order to increase immune function and combat the infection. Whether social animals that collect food for their brood or nestmates adjust their nutrient intake to the infection states of their social partners is virtually unexplored. Here we devel...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2017.08.005
更新日期：2018-04-01 00:00:00
abstract：:The potato leafhopper, Empoasca fabae, is a pest of economic crops in the United States and Canada, where it causes damage known as hopperburn. Saliva, along with mechanical injury, leads to decreases in gas exchange rates, stunting and chlorosis. Although E. fabae saliva is known to induce plant responses, little kno...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2012.10.002
更新日期：2012-12-01 00:00:00
abstract：:Electroantennogram (EAG) responses to the aphid sex pheromone components, (1R,4aS,7S,7aR)-nepetalactol and (4aS,7S,7aR)-nepetalactone, and a plant volatile, (E)-2-hexenal, were investigated at three different positions (5/6th, 4/5th and 3/4th inter-segmental regions) along the antennae of four different morphs in two ...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(02)00082-3
更新日期：2002-05-01 00:00:00
abstract：:Butterfly wing color-patterns are a phenotypically coordinated array of scales whose color is determined as cellular interpretation outputs for morphogenic signals. Here we investigated distribution patterns of scale shape and size in relation to position and coloration on the hindwings of a nymphalid butterfly Junoni...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2008.11.006
更新日期：2009-02-01 00:00:00
abstract：:The hemocytes phagocytosis in response to microorganisms may play an important role in the cellular immune responses of insects. Here, we have evaluated the effects of the platelet-activating factor (PAF) and eicosanoids in the phagocytosis of hemocyte monolayers of Rhodnius prolixus to the yeast Saccharomyces cerevis...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2007.10.004
更新日期：2008-02-01 00:00:00
abstract：:Orcokinins (OKs) are neuropeptides that were first identified in crustacean through their myotropic activity. In insects, the OK gene gives rise to two mRNAs coding for two different families of conserved mature neuropeptides: OKA and OKB. Although OKs are conserved in many insect species, its physiological role in th...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2015.10.002
更新日期：2015-11-01 00:00:00
abstract：:We examined the behavioural and electrophysiological responses of taiga ticks (Ixodes persulcatus) to several olfactory stimuli: Osmopherone® (5-a-androst-16-en-3-ol), Osmopherine® (butanoic and 3-methylbutanoic acids), DEET® (N,N-diethyl-meta-toluamide), ethanol (96%), and water (control stimulus). To study individua...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2012.04.004
更新日期：2012-07-01 00:00:00
abstract：:The corpora cardiaca (CC) of the Italian race (including also the africanised variety) of the honeybee (Apis mellifera ligustica) contain approximately 3 pmol of a hypertrehalosaemic peptide. This peptide is identical in structure to the adipokinetic hormone (AKH) found in Manduca sexta, Mas-AKH. The CC of the dark Eu...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(98)00158-9
更新日期：1999-07-01 00:00:00
abstract：:The rice brown planthopper, Nilaparvata lugens, is an important migratory pest in many rice planting areas of Asia. The typical wing dimorphism of N. lugens gives them flexibility to adapt to different environmental cues. As an important hormone in the insect's endocrine regulation, juvenile hormone (JH) has previousl...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2017.08.012
更新日期：2017-10-01 00:00:00
abstract：:Next generation sequencing has revealed the widespread occurrence of persistent virus infections in insects but little is known regarding to what extent persistent infections can affect cellular physiology and how they might contribute to the development of disease. In contrast to the pathogenic infections occurring i...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2019.03.004
更新日期：2019-01-01 00:00:00
abstract：:Facultative diapause, a strategy that allows insects to initiate additional generations when conditions are favorable or to enter diapause when they are not, has a profound effect on the ecology and evolution of species. Most previous studies have concentrated on the role of photoperiod and temperature in inducing fac...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2010.08.007
更新日期：2010-12-01 00:00:00
abstract：:Dippu-allatostatins (ASTs) have pleiotropic effects in Locusta migratoria. Dippu-ASTs act as releasing factors for adipokinetic hormone I (AKH I) from the corpus cardiacum (CC) and also alter juvenile hormone (JH) biosynthesis and release from the corpus allatum (CA). Dippu-AST-like immunoreactivity is found within la...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2008.03.007
更新日期：2008-06-01 00:00:00
abstract：:Temperature can modulate the responses of ectotherms to environmental stressors, such as food shortage. Temperature-mediated plasticity in starvation resistance can arise by changes in the amount of energy stored, the speed of energy expenditure, or the threshold energy reserves required for survival. However, few stu...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2018.08.004
更新日期：2018-10-01 00:00:00
abstract：:In the honeybee Apis mellifera, a sting pheromone produced by sting glands plays an important role in coordinating defensive behavior. This pheromone is a blend constituted by several components. Little is known about the neural substrates underlying sting pheromone processing in the bee brain. Here, we investigated t...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2008.03.004
更新日期：2008-05-01 00:00:00
