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Expression profiling of a diapause-specific peptide (DSP) of the leaf beetle Gastrophysa atrocyanea and silencing of DSP by double-strand RNA.

Abstract：

:A diapause-specific peptide (DSP) composed of 41 amino acid residues including 6 cysteines, has been isolated from diapausing adults of the leaf beetle Gastrophysa atrocyanea. In this study, DSP was found to be expressed primarily in diapausing adults and to a minor extent in pupae, but not in eggs, larvae, or post-diapausing adults. DSP was not induced by bacterial or fungal challenge. DSP-less adults were generated by the injection of double-stranded RNA (dsRNA) corresponding to the dsp gene into pre-diapausing adults. Gene silencing induced by dsRNA was found to be a useful tool for the analysis of DSP in diapausing adults. DSP-less adults showed similar burrowing behavior and oxygen consumption as control insects suggesting that DSP is not essential for the normal onset and maintenance of diapause.

journal_name

J Insect Physiol

journal_title

Journal of insect physiology

authors

Tanaka H,Suzuki K

doi

10.1016/j.jinsphys.2005.03.018

keywords:

subject

Has Abstract

pub_date

2005-06-01 00:00:00

pages

701-7

issue

6

eissn

0022-1910

issn

1879-1611

pii

S0022-1910(05)00081-8

journal_volume

51

pub_type

杂志文章

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