Abstract：
:The recently available genome of Aedes albopictus - the most worldwide-spread human arbovirus vector - has revealed a large genome repertory and a great plasticity which are believed to have contributed to the species success as an invasive species and opened the way to genomic, transcriptomic and proteomic studies. We carried out the first wide-scale quantitative proteomic analysis of Ae. albopictus female head and thorax by means of a 'shotgun' approach based on nano liquid chromatography-high resolution mass spectrometry associated to protein Label Free Quantification (LFQ) which allows to assess differences in protein expression between tissues and different physiological stages. We identified 886 and 721 proteins in heads and thoraxes respectively, 5 of which were exclusively expressed in thoraxes and 170 in heads, consistently with the more complex head physiology. Head-protein expression was found to be highly divergent between virgin and mated females and limited before and after blood-feeding and oviposition. The large repertoire of proteins identified represents an instrumental source of data for genome annotation and gene-expression studies, and may contribute to studies aimed at investigating the molecular bases of physiological processes of this successful invasive species.
journal_name
J Insect Physioljournal_title
Journal of insect physiologyauthors
Iovinella I,Caputo B,Della Torre A,Dani FRdoi
10.1016/j.jinsphys.2017.03.005subject
Has Abstractpub_date
2017-05-01 00:00:00pages
33-38eissn
0022-1910issn
1879-1611pii
S0022-1910(16)30384-5journal_volume
99pub_type
杂志文章abstract：:The intracellular elemental concentrations of K, Na, Cl, P, Mg and Ca within Type I cells of the Malpighian tubules of Locusta migratoria have been measured using electron probe X-ray microanalysis. The distribution of Na, K and Cl was not homogeneous within the cells and concentration gradients exist from basal to ap...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(00)00131-1
更新日期：2001-04-01 00:00:00
abstract：:Ultrastructural and electrophysiological (single-cell recordings) investigations were carried out on the coeloconic sensilla borne by the apical antenna of the larvae of Libellula depressa (Odonata: Libellulidae). These sensilla appear as pegs located in pits. One of them is a compound sensillum constituted of two fus...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2007.02.006
更新日期：2007-06-01 00:00:00
abstract：:We have investigated the effect of the locust myosuppressin, SchistoFLRFamide, on the activity of amylase and alpha-glucosidase in the midgut of 2-week old male locusts. Total enzyme activity in the lumen contents and tissue extracts of midguts responds to SchistoFLRFamide in a dose-dependent manner that appears to va...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2004.10.003
更新日期：2005-01-01 00:00:00
abstract：:Ultraviolet (UV) radiation produces reactive oxygen species (ROS) in mammals, where melatonin plays the role of a ROS scavenger. The melatonin synthetic enzyme arylalkylamine N-acetyltransferase (NAT) is a significant element in a possible ROS removal system. Changes in NAT activity and melatonin content were determin...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2008.06.005
更新日期：2008-07-01 00:00:00
abstract：:The cabbage beetle, Colaphellus bowringi Baly, is a short-day species that has both a facultative summer and winter adult diapause. In this study, we systematically investigated the effects of parental physiological age, mating pattern (including mating frequency and delayed mating), and diapause duration on the diapa...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2007.02.015
更新日期：2007-09-01 00:00:00
abstract：:Female grasshoppers of acoustically communicating species assume series of reproductive states that are associated with particular behaviours. Studies on laboratory populations of Chorthippus biguttulus (L.) revealed that females of this species lack the period of 'passive copulatory readiness', increase their attract...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2010.01.006
更新日期：2010-07-01 00:00:00
abstract：:We found that in the flesh fly Neobellieria bullata, vitellogenesis can be inhibited in a dose-dependent way by two injections of 60 microg MK-801/g body mass. In the desert locust Schistocerca gregaria, vitellogenesis can also be fully inhibited but only by repeated injections of 200-400 microg/g body mass. In this s...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2004.07.003
更新日期：2004-10-01 00:00:00
abstract：:Diapause is an alternate developmental pathway that is regulated by the neuroendocrine system in insects. To date, much of the information that has been published regarding the possible molecular events associated with diapause have been at the level of transcription. However, since transcription and translation are n...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2011.03.022
更新日期：2011-05-01 00:00:00
abstract：:Antimicrobial peptides (AMPs) are conserved cationic peptides which act both as defense molecules of the host immune system and as regulators of the commensal microbiome. Expression of AMPs is induced in response to infection by the Toll and Imd pathway. Under non-infected conditions, the transcription factor dFOXO di...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2014.04.006
更新日期：2014-10-01 00:00:00
abstract：:Facultative diapause, a strategy that allows insects to initiate additional generations when conditions are favorable or to enter diapause when they are not, has a profound effect on the ecology and evolution of species. Most previous studies have concentrated on the role of photoperiod and temperature in inducing fac...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2010.08.007
更新日期：2010-12-01 00:00:00
abstract：:Insects and vertebrates have multiple major physiological systems, each species having a circulatory system, a metabolic system, and a respiratory system that enable locomotion and survival in stressful environments, among other functions. Broadening our understanding of the physiology of Drosophila melanogaster requi...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2017.03.004
更新日期：2017-05-01 00:00:00
abstract：:The adrenergic agonists octopamine, tyramine and clonidine inhibited the normal pheromonotropic action due to PBAN (pheromone biosynthesis activating neuropeptide) in incubations of intersegmental tissues that are situated between the 8th and 9th abdominal segments of the moth ovipositor tip. This inhibition was rever...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(96)00088-1
更新日期：1997-03-01 00:00:00
abstract：:Nestmate recognition in ants is based on perceived differences in a multi-component blend of hydrocarbons that are present on the insect cuticle. Although supplementation experiments have shown that some classes of hydrocarbons, such as methyl branched alkanes and alkenes, have a salient role in nestmate recognition, ...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2011.10.010
更新日期：2012-01-01 00:00:00
abstract：:Aquatic insects play critical roles in freshwater ecosystems and temperature is a fundamental driver of species performance and distributions. However, the physiological mechanisms that determine the thermal performance of species remain unclear. Here we used a metabolomics approach to gain insights into physiological...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2017.07.008
更新日期：2017-08-01 00:00:00
abstract：:It is well known that viscosity reduces the intake rates in nectar-feeding insects, such as nectivorous ants, though it remains unclear whether viscosity imposes a higher energy investment in these insects, and how this affects their feeding motivation. To address this issue, we studied feeding behavior, metabolism, a...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2020.104164
更新日期：2021-01-01 00:00:00
abstract：:Elucidating genes that affect life span or that can be used as biomarkers for ageing has received attention in diverse studies in recent years. Using model organisms and various approaches several genes have been linked to the longevity phenotype. For Drosophila melanogaster those studies have usually focussed on one ...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2013.01.005
更新日期：2013-03-01 00:00:00
abstract：:A two-step HPLC purification procedure resulted in a factor from the stable fly that depolarizes the lumen-negative transepithelial voltage (V(t)) of the adult stable fly Malpighian tubule. When applied to tubules of the female mosquito, Aedes aegypti, this factor partially mimics the electrophysiological actions of t...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(97)00078-4
更新日期：1997-10-01 00:00:00
abstract：:Dopamine is a potential integrator between the central nervous system and reproductive system in insects. To test for a possible action of dopamine on the male reproductive organ via hemolymph in honey bees, relative expression levels of dopamine receptor genes and second messenger levels responding to dopamine in the...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2018.11.005
更新日期：2019-01-01 00:00:00
abstract：:To develop inside their insect hosts, endoparasitoid wasps must either evade or overcome the host's immune system. Several ichneumonid and braconid wasps inject polydnaviruses that display well-studied immune suppressive effects. However, little is known about the strategies of immunoevasion used by other parasitoid f...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(03)00054-4
更新日期：2003-05-01 00:00:00
abstract：:In Oncopeltus fasciatus, evidence shown here indicates it is calmodulin (CaM) that activates phospholipase-C (PLC), beginning a signalling pathway necessary for endocytic uptake of yolk precursor molecules. Epithelial cell-produced CaM, transported to oocytes via gap junctions, has been shown to be required for recept...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2010.04.006
更新日期：2010-09-01 00:00:00
abstract：:We studied male locomotory response to trails and patches of sex pheromone (left respectively by free-ranging females and females constrained to stay on a small area) in the two parasitoids Aphelinus asychis (Hymenoptera: Aphelinidae) and Trichogramma brassicae (Hymenoptera: Trichogrammatidae). Under the hypothesis th...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(97)00172-8
更新日期：1998-07-01 00:00:00
abstract：:In a Japanese population of Locusta migratoria, adult females become reproductively inactive under crowding and long days (LD) and reproductively active under crowding and short days (SD). The identity and titre of ecdysteroids in the haemolymph and ovaries from adult females reared under SD and LD were investigated b...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(02)00058-6
更新日期：2002-04-01 00:00:00
abstract：:Next generation sequencing has revealed the widespread occurrence of persistent virus infections in insects but little is known regarding to what extent persistent infections can affect cellular physiology and how they might contribute to the development of disease. In contrast to the pathogenic infections occurring i...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2019.03.004
更新日期：2019-01-01 00:00:00
abstract：:Heat is the principal host-associated cue for the blood-sucking bug Rhodnius prolixus. It is both necessary and sufficient to trigger the "proboscis extension response" (PER), an essential element of the feeding behaviour of this insect. The aim of this study was to determine whether the temperature of an object itsel...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2011.07.012
更新日期：2011-10-01 00:00:00
abstract：:In nature adult insects, such as parasitic wasps or 'parasitoids' often depend on supplemental nutritional sources, such as sugars and other carbohydrates, to maximize their life-expectancy and reproductive potential. These food resources are commonly obtained from animal secretions or plant exudates, including honeyd...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2012.03.002
更新日期：2012-06-01 00:00:00
abstract：:The presence of two spectral mechanisms, near-ultraviolet and green (lambda(max)=545nm), is strongly suggested by electroretinographic visual spectral sensitivity curves obtained under dark and red chromatic adaptation conditions in the compound eyes of the click beetle Pyrophorus punctatissimus. The bioluminescence e...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(99)00224-3
更新日期：2000-07-01 00:00:00
abstract：:Octopamine plays a major role in insect motor control and is released from dorsal unpaired median (DUM) neurones, a group of cells located on the dorsal midline of each ganglion. We were interested whether and how these neurones are activated during walking and chose the semi-intact walking preparation of stick insect...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2007.08.010
更新日期：2008-01-01 00:00:00
abstract：:Juvenile hormone (JH) produced by the corpus allatum (CA) stimulates vitellogenesis and reduces the synthesis of hexamerin proteins in adult females of Pyrrhocoris apterus. At present it is unknown whether the signaling pathway involving the JH receptor gene Methoprene tolerant (Met) and its binding partner Taiman (Ta...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2016.08.009
更新日期：2016-01-01 00:00:00
abstract：:Apis mellifera treated during different developmental phases with pyriproxyfen, a juvenile hormone analogue, show profound alterations in cuticular pigmentation and sclerotization. When the treatment is effected during the feeding phase of the fifth larval instar (LF5), the pupal development is blocked and pigmentatio...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(97)00113-3
更新日期：1998-05-01 00:00:00
abstract：:RNA interference has been successfully used in adult honeybees, but there are only few reports about abdominal application of dsRNA/siRNA which have reached more distant tissues than the fat body. We studied systemic RNAi in honeybees by injecting fluorescent siRNA of the ubiquitously expressed honeybee homologue of t...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2011.03.013
更新日期：2011-07-01 00:00:00
