当前位置： SCI文献检索 > JOURNAL OF INSECT PHYSIOLOGY期刊下所有文献 > Systemic RNA-interference in the honeybee Apis mellifera: tissue dependent uptake of fluorescent siRNA after intra-abdominal application observed by laser-scanning microscopy.

Systemic RNA-interference in the honeybee Apis mellifera: tissue dependent uptake of fluorescent siRNA after intra-abdominal application observed by laser-scanning microscopy.

Abstract：

:RNA interference has been successfully used in adult honeybees, but there are only few reports about abdominal application of dsRNA/siRNA which have reached more distant tissues than the fat body. We studied systemic RNAi in honeybees by injecting fluorescent siRNA of the ubiquitously expressed honeybee homologue of the Glycerol-3-Phosphate Dehydrogenase (amGpdh) into the abdomens of adult bees and followed them by laser scanning microscopy and qPCR. The fat body was the sole tissue emitting fluorescence and showing a decreased gene expression, whereas the siRNA had apparently not reached the other tissues. Therefore, we conclude that certain genes in other tissues than the fat body cannot be easily reached by injecting siRNA into the body cavity. In particular, the lack of amGpdh knock down in ovaries after amGpdh dsRNA injection, supports that in some cases it may be particularly difficult to interfere with gene expression in ovaries by intra-abdominal injection. In these cases alternative inhibition techniques may be required to achieve an organismic non-lethal disruption of transcription.

journal_name

J Insect Physiol

journal_title

Journal of insect physiology

authors

Jarosch A,Moritz RF

doi

10.1016/j.jinsphys.2011.03.013

subject

Has Abstract

pub_date

2011-07-01 00:00:00

pages

851-7

issue

7

eissn

0022-1910

issn

1879-1611

pii

S0022-1910(11)00081-3

journal_volume

57

pub_type

杂志文章

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