Abstract：
:In Oncopeltus fasciatus, evidence shown here indicates it is calmodulin (CaM) that activates phospholipase-C (PLC), beginning a signalling pathway necessary for endocytic uptake of yolk precursor molecules. Epithelial cell-produced CaM, transported to oocytes via gap junctions, has been shown to be required for receptor-mediated endocytic uptake of vitellogenins (Vgs, the protein precursors of yolk). To determine if CaM was directly or indirectly stimulating the phospholipase-C (PLC) signalling cascade and thus controlling Vg endocytosis we used a series of molecules known to inactivate various elements of the pathway. W-7 prevents CaM from interacting with other molecules. Neomycin isolates PIP(2) from PLC. U-73122 directly inactivates PLC. 2-APB blocks IP(3) receptors which would otherwise cause release of Ca(2+). Verapamil and CdCl(2) block Ca(2+) release channels. Staurosporin and calphostin are inhibitors of PK-C. 1-Hexadecyl-2-acetyl glycerol (HAG) binds to diacylglycerol (DAG). Through the use of these antagonists we show here that: (1) the activation of phospholipase-C in this system requires CaM. (2) Stimulated phospholipase-C converts PIP(2) into IP(3) and DAG. (3) IP(3) causes increase in cytosolic Ca(2+). (4) DAG and Ca(2+) each stimulate phosphokinase-C, resulting in endocytosis of Vgs.
journal_name
J Insect Physioljournal_title
Journal of insect physiologyauthors
Brown PT,Herbert P,Woodruff RIdoi
10.1016/j.jinsphys.2010.04.006subject
Has Abstractpub_date
2010-09-01 00:00:00pages
1300-5issue
9eissn
0022-1910issn
1879-1611pii
S0022-1910(10)00122-8journal_volume
56pub_type
杂志文章abstract：:Egg-laying in Locusta migratoria involves the control of a variety of complex behavioural patterns including those that regulate digging of the oviposition hole and retention of eggs during digging. These two behavioural patterns are under the control of central pattern generators (CPGs). The digging and egg-retention...
journal_title：Journal of insect physiology
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doi：10.1016/j.jinsphys.2014.02.002
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pub_type： 杂志文章
doi：10.1016/s0022-1910(02)00252-4
更新日期：2003-01-01 00:00:00
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journal_title：Journal of insect physiology
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更新日期：2007-03-01 00:00:00
abstract：:The major juvenile hormone (JH) homolog synthesized in vitro by the adult female Medfly (Ceratitis capitata) corpus allatum (CA) is JHB(3), with JH-III the minor homolog. Methyl-incorporation in vitro in post-eclosion virgin females is age-dependent. Basal activity occurs during the first four days post-eclosion and i...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(03)00047-7
更新日期：2003-06-01 00:00:00
abstract：:The quantity and composition of the six major mandibular gland components of young queenless workers of the Cape honeybee (Apis mellifera capensis) were determined. The total amount of the six components increased with age. The relative quantities in the mandibular gland secretion of queenless caged workers were found...
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pub_type： 杂志文章
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更新日期：2001-07-01 00:00:00
abstract：:Odorant binding proteins (OBPs) contribute to the remarkable sensitivity of the insect's olfactory system and play an important role in insect chemical communication. In this study, we identified 11 putative cDNAs encoding OBPs (namely SexiOBP1-11) from the antennal full length cDNA library of the beet armyworm Spodop...
journal_title：Journal of insect physiology
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更新日期：2013-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.jinsphys.2009.05.010
更新日期：2009-09-01 00:00:00
abstract：:MicroRNAs (miRNA) participate in regulating diverse biological pathways by translational repression in animals. They have attracted increasing attention recently. However, little work has been done on the miRNA genes in agriculturally important pests. Because the transcripts of most miRNA genes are the products of typ...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2010.07.007
更新日期：2010-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2018-01-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2004.03.007
更新日期：2004-06-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2005.04.009
更新日期：2005-08-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(97)00117-0
更新日期：1998-11-01 00:00:00
abstract：:Qualitative and quantitative changes in haemolymph proteins in Heliothis virescens were observed in larvae injected with either Microplitis croceipes teratocytes or teratocyte secreted proteins (TSP). Haemolymph protein titres in hosts receiving either 0.5 or 1 larval equivalent (LE) of teratocytes were similar to tho...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(96)00118-7
更新日期：1997-06-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2016.08.009
更新日期：2016-01-01 00:00:00
abstract：:Dippu-allatostatins (ASTs) have pleiotropic effects in Locusta migratoria. Dippu-ASTs act as releasing factors for adipokinetic hormone I (AKH I) from the corpus cardiacum (CC) and also alter juvenile hormone (JH) biosynthesis and release from the corpus allatum (CA). Dippu-AST-like immunoreactivity is found within la...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2008.03.007
更新日期：2008-06-01 00:00:00
abstract：:Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) is a worldwide emerging pest of soft fruits, but its cold tolerance has not been thoroughly explored. We determined the cold tolerance strategy, low temperature thermal limits, and plasticity of cold tolerance in both male and female adult D. suzukii. We reared f...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2015.05.003
更新日期：2015-08-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
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更新日期：2012-08-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2014.08.006
更新日期：2014-11-01 00:00:00
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更新日期：2002-09-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
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更新日期：1999-05-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2013.07.006
更新日期：2013-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.jinsphys.2009.05.002
更新日期：2009-08-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2014.03.003
更新日期：2014-05-01 00:00:00
abstract：:Earlier experiments demonstrated a strong up-regulation of per mRNA in wild-type (Wt) females of Pyrrhocoris apterus reared under diapause-inducing short days, while per mRNA levels were low in females of two non-diapause mutant strains (Nd), irrespective of photoperiod. In the present study, different sequences of pe...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2005.01.009
更新日期：2005-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.jinsphys.2007.03.004
更新日期：2007-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：2002-02-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2018.04.010
更新日期：2018-01-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2017.09.001
更新日期：2017-10-01 00:00:00
abstract：:The in vitro secretion of ecdysteroids from the prothoracic glands of larvae of Gryllus bimaculatus was analysed by HPLC-RIA. The primary product was identified as 3-dehydroecdysone (65-93%), with lesser amounts of ecdysone (7-35%). Production and release of ecdysteroids from the prothoracic glands are calcium-depende...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(98)00057-2
更新日期：1998-10-01 00:00:00
