Abstract：
:MicroRNAs (miRNA) participate in regulating diverse biological pathways by translational repression in animals. They have attracted increasing attention recently. However, little work has been done on the miRNA genes in agriculturally important pests. Because the transcripts of most miRNA genes are the products of type-II RNA polymerase, pri-miRNA has a poly(A) tail and appears in expressed sequence tags (EST). We developed a computational pipeline to identify miRNA genes from insect ESTs. First, 980,697 ESTs from 63 insects were collected and used to search the nr database. The ESTs which did not share significant similarities with any known protein-coding genes were treated as non-coding ESTs. Next, known mature miRNAs were used to align with non-coding ESTs. The ESTs which contain the sequence of mature miRNA were treated as candidate ESTs. Finally, putative precursors were extracted flanking the mature miRNA region in candidate ESTs and evaluated by the Triplet-SVM algorithm. As a result, 86 miRNAs from 30 insect species were found based on a strict criterion while 330 miRNAs from 51 species were found based on a loose criterion. Evolution analysis indicated that mir-467, mir-297 and mir-466 were the highest conserved miRNA families in insects. To confirm the reliability of putative insect miRNAs, the expression profile of nine predicted miRNAs in Locusta migratoria was investigated. Eight miRNAs were successfully detected by RT-PCR. Most miRNAs were expressed ubiquitously at all examined tissues and developmental stages whereas Lmi-mir-509 was specifically expressed in the thorax of the 2nd, 4th and 5th instars and adult locust. In all, our work reported an efficient computational strategy for predicting miRNA genes from insect ESTs and presented tens of miRNAs in diverse insect species which are expected to participate in many important physiological processes.
journal_name
J Insect Physioljournal_title
Journal of insect physiologyauthors
Jia Q,Lin K,Liang J,Yu L,Li Fdoi
10.1016/j.jinsphys.2010.07.007subject
Has Abstractpub_date
2010-12-01 00:00:00pages
1763-9issue
12eissn
0022-1910issn
1879-1611pii
S0022-1910(10)00218-0journal_volume
56pub_type
杂志文章abstract：:Qualitative and quantitative changes in haemolymph proteins in Heliothis virescens were observed in larvae injected with either Microplitis croceipes teratocytes or teratocyte secreted proteins (TSP). Haemolymph protein titres in hosts receiving either 0.5 or 1 larval equivalent (LE) of teratocytes were similar to tho...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(96)00118-7
更新日期：1997-06-01 00:00:00
abstract：:Thermal tolerance, supercooling point, water balance and osmoregulatory ability of Pringleophaga marioni Viette (Lepidoptera: Tineidae) are investigated in this study. Field-fresh larvae had a mean CT(Min) (cold stupor) of -0.6 degrees C and a mean CT(Max) (heat coma) of 38.7 degrees C. The mean supercooling point of ...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(97)00001-2
更新日期：1997-07-01 00:00:00
abstract：:Ultraviolet (UV) radiation produces reactive oxygen species (ROS) in mammals, where melatonin plays the role of a ROS scavenger. The melatonin synthetic enzyme arylalkylamine N-acetyltransferase (NAT) is a significant element in a possible ROS removal system. Changes in NAT activity and melatonin content were determin...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2008.06.005
更新日期：2008-07-01 00:00:00
abstract：:The way energy resources are used under variable environmental conditions lies at the heart of our understanding of resource management and opportunism in many organisms. Here we sought to determine how a time-limited, synovigenic and polyphagous insect with a high reproductive-potential (Anastrephaludens), copes beha...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2011.08.001
更新日期：2011-12-01 00:00:00
abstract：:Haemolymph calcium homeostasis in insects is achieved through the regulation of calcium excretion by Malpighian tubules in two ways: (1) sequestration of calcium within biomineralized granules and (2) secretion of calcium in soluble form within the primary urine. Using the scanning ion-selective electrode technique (S...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2016.01.005
更新日期：2016-04-01 00:00:00
abstract：:Haemocytes play crucial roles in immune responses and survival in insects. Specific cell markers have proven effective in clarifying the function and haematopoiesis of haemocytes. The silkworm Bombyx mori is a good model for studying insect haemocytes; however, little is known about haemocyte-specific markers or their...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2014.10.007
更新日期：2014-12-01 00:00:00
abstract：:Juvenile hormone (JH) produced by the corpus allatum (CA) stimulates vitellogenesis and reduces the synthesis of hexamerin proteins in adult females of Pyrrhocoris apterus. At present it is unknown whether the signaling pathway involving the JH receptor gene Methoprene tolerant (Met) and its binding partner Taiman (Ta...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2016.08.009
更新日期：2016-01-01 00:00:00
abstract：:Ultrastructural and electrophysiological (single-cell recordings) investigations were carried out on the coeloconic sensilla borne by the apical antenna of the larvae of Libellula depressa (Odonata: Libellulidae). These sensilla appear as pegs located in pits. One of them is a compound sensillum constituted of two fus...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2007.02.006
更新日期：2007-06-01 00:00:00
abstract：:The clock protein PERIOD (PER) displays circadian cycles of accumulation, phosphorylation, nuclear translocation and degradation in Drosophila melanogaster clock cells. One exception to this pattern is in follicular cells enclosing previtellogenic ovarian egg chambers. In these cells, PER remains high and cytoplasmic ...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2009.01.017
更新日期：2009-05-01 00:00:00
abstract：:Helicoverpa armigera (Lepidoptera, Noctuidae) is an important agricultural pest with a pupal diapause. Cytochrome c oxidase (COX) is a key speed-limited enzyme of oxidative phosphorylation in mitochondria for ATP production. A differentially expressed cDNA fragment encoding COX subunit 1 (cox1) was cloned by different...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2010.08.023
更新日期：2010-12-01 00:00:00
abstract：:Males transfer sperm, proteins and other molecules to females during mating. In Drosophila melanogaster, these molecules contribute to the induction of egg maturation, ovulation, oviposition, sperm storage and changes in female receptivity. This suite of physiological and behavioral changes is referred to as the femal...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2017.04.004
更新日期：2017-05-01 00:00:00
abstract：:Nestmate recognition in ants is based on perceived differences in a multi-component blend of hydrocarbons that are present on the insect cuticle. Although supplementation experiments have shown that some classes of hydrocarbons, such as methyl branched alkanes and alkenes, have a salient role in nestmate recognition, ...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2011.10.010
更新日期：2012-01-01 00:00:00
abstract：:Multiple genetic and environmental factors interact to influence starvation resistance, which is an important determinant of fitness in many organisms, including Drosophila melanogaster. Recent studies have revealed that mating can alter starvation resistance in female D. melanogaster, but little is known about the be...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2015.09.002
更新日期：2015-11-01 00:00:00
abstract：:The regulation of caste differentiation is essential to insect eusociality. Termite soldiers are sterile and cannot eat by themselves because they have specialized mouth morphology. Almost all termite species have a soldier caste, and the soldier ratio per colony is maintained at a low level, probably by elaborate reg...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2011.03.005
更新日期：2011-06-01 00:00:00
abstract：:The photonegative response to light stimulation in Rhodnius prolixus (Reduviidae, Triatominae) is modulated by compound eyes and ocelli. Screening pigments in the visual system have been shown to protect the cellular structures from the oxidative stress damage produced by blood ingestion and light stimulation. Red-eye...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2017.06.011
更新日期：2017-08-01 00:00:00
abstract：:To counteract water loss due to excretion, cuticular transpiration and respiration, various groups of arthropods have developed mechanisms for active uptake of water vapor from unsaturated air. In this study, active uptake capabilities and water loss rates were examined in the various developmental stages of the cat f...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(03)00153-7
更新日期：2003-12-01 00:00:00
abstract：:The pierid butterfly Colias croceus (Geoffroy in Fourcroy, 1785), established in the Azores archipelago, is polymorphic with six forms, C. croceus f. croceus ♂ and ♀, C. c. f. cremonae ♂ and ♀, C. c. f. helice ♀, and C. c. f. cremonaehelice ♀. We investigated the optical mechanisms underlying the wing colouration of t...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2020.104114
更新日期：2020-01-01 00:00:00
abstract：:To determine whether ecdysteroids are associated with reproductive division of labor in Bombus terrestris, we measured their levels in hemolymph and ovaries of queens and workers. Queens heading colonies had large active ovaries with high ecdysteroid content, whereas virgin gynes and mated queens before and after diap...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2005.03.009
更新日期：2005-07-01 00:00:00
abstract：:Blood-sucking insects strongly rely on olfactory cues to find their vertebrate hosts. As in other insects with different lifestyles, it has been shown that endogenous and exogenous factors modulate olfactory responses. The triatomine bug Rhodnius prolixus is an important vector of Chagas disease and a classical model ...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2014.09.009
更新日期：2014-12-01 00:00:00
abstract：:Disruption of the appropriate balance between juvenile hormone (JH) and ecdysteroids causes abnormal insect development. The application of a JH analog (JHA) during the early days of the final (fifth) instar induces dauer larvae with low ecdysteroid titers in insects, but the mechanism that underlies the action of JHA...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2015.03.012
更新日期：2015-09-01 00:00:00
abstract：:We have examined the relative sensitivity of Aedes albopictus C7-10 mosquito cells to irradiation with ultraviolet light from a germicidal lamp. On the basis of plating efficiency, C7-10 cells were approximately two times more resistant to UV light than human 293 leukemia cells. Recovery after UV irradiation was accom...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(01)00156-1
更新日期：2002-01-01 00:00:00
abstract：:Apis mellifera treated during different developmental phases with pyriproxyfen, a juvenile hormone analogue, show profound alterations in cuticular pigmentation and sclerotization. When the treatment is effected during the feeding phase of the fifth larval instar (LF5), the pupal development is blocked and pigmentatio...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(97)00113-3
更新日期：1998-05-01 00:00:00
abstract：:In this study we probe the molecular events underpinning diapause observed in overwintering females of Culex pipiens. Using suppressive subtractive hybridization (SSH) we have identified 40 genes that are either upregulated or downregulated during this seasonal period of dormancy. Northern blot hybridizations have con...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2006.08.008
更新日期：2007-03-01 00:00:00
abstract：:Drosophila suzukii is an invasive pest that prefers to lay eggs in ripening fruits, whereas most closely related Drosophila species exclusively use rotten fruit as oviposition site. This behaviour is allowed by an enlarged and serrated ovipositor that can pierce intact fruit skin, and by multiple contact sensory syste...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2020.104088
更新日期：2020-01-01 00:00:00
abstract：:When non-diapause and diapause pupae of Delia antiqua were exposed to various thermoperiods where thermophase (T) was 25 degrees C and the cryophase (C) was 15 or 20 degrees C (TC(15) or TC(20)) in constant darkness (DD), the majority of both types of flies emerged before the rise in temperature. Eclosion time was del...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(02)00157-9
更新日期：2002-09-01 00:00:00
abstract：:In independent assays, workers of the ant Camponotus mus were conditioned to visit an arena where they found a large drop of sucrose solution of different concentrations, from 5 to 70% weight on weight (w/w). Single ants were allowed to collect the sucrose solution ad libitum, and feeding time, feeding interruptions, ...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(98)00053-5
更新日期：1998-07-01 00:00:00
abstract：:Force feeding of RH-5992 (Tebufenozide), a non-steroidal ecdysone agonist to newly moulted sixth instar larvae of the spruce budworm, Choristoneura fumiferana, (Lepidoptera: Tortricidae) initiates a precocious, incomplete moult. Within 6h post treatment (pt) the larva stops feeding and remains quiescent. Around 12hpt,...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(96)00062-5
更新日期：1997-02-19 00:00:00
abstract：:The flightless bug Pyrrhocoris apterus (L.) is polymorphic for both wing length and flight muscle development. The developed flight muscles of macropterous adults of both sexes first enlarge their volume during the first 5 days after adult emergence, but are then histolyzed in all males and females older than 10 and 1...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2005.10.009
更新日期：2006-03-01 00:00:00
abstract：:Honeybee drinking is facilitated by a "mop-like" tongue, which helps honeybees suck in the sucrose solution from the environment. However, the liquid-transport mechanism from the pharynx to the crop, especially the natural link between abdominal pumping and dipping behavior on the sucrose solution intake, remains obsc...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2018.11.002
更新日期：2019-01-01 00:00:00
abstract：:Following a short swarming flight, winged adults of the Formosan subterranean termite, Coptotermes formosanus lose their wings and form tandem pairs. These dealates or primary reproductives then form incipient colonies. Topical application of 5 microg of the non-steroidal ecdysone agonist RH-0345 (halofenozide) in 0.1...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(03)00069-6
更新日期：2003-07-01 00:00:00
