Diapause-specific gene expression in the northern house mosquito, Culex pipiens L., identified by suppressive subtractive hybridization.

Abstract:

:In this study we probe the molecular events underpinning diapause observed in overwintering females of Culex pipiens. Using suppressive subtractive hybridization (SSH) we have identified 40 genes that are either upregulated or downregulated during this seasonal period of dormancy. Northern blot hybridizations have confirmed the expression of 32 of our SSH clones, including six genes that are upregulated specifically in early diapause, 17 that are upregulated in late diapause, and two upregulated throughout diapause. In addition, two genes are diapause downregulated and five remain unchanged during diapause. These genes can be categorized into eight functional groups: genes with regulatory functions, metabolically-related genes, those involved in food utilization, stress response genes, cytoskeletal genes, ribosomal genes, transposable elements, and genes with unknown functions.

journal_name

J Insect Physiol

authors

Robich RM,Rinehart JP,Kitchen LJ,Denlinger DL

doi

10.1016/j.jinsphys.2006.08.008

subject

Has Abstract

pub_date

2007-03-01 00:00:00

pages

235-45

issue

3

eissn

0022-1910

issn

1879-1611

pii

S0022-1910(06)00151-X

journal_volume

53

pub_type

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