Abstract:
:In this study we probe the molecular events underpinning diapause observed in overwintering females of Culex pipiens. Using suppressive subtractive hybridization (SSH) we have identified 40 genes that are either upregulated or downregulated during this seasonal period of dormancy. Northern blot hybridizations have confirmed the expression of 32 of our SSH clones, including six genes that are upregulated specifically in early diapause, 17 that are upregulated in late diapause, and two upregulated throughout diapause. In addition, two genes are diapause downregulated and five remain unchanged during diapause. These genes can be categorized into eight functional groups: genes with regulatory functions, metabolically-related genes, those involved in food utilization, stress response genes, cytoskeletal genes, ribosomal genes, transposable elements, and genes with unknown functions.
journal_name
J Insect Physioljournal_title
Journal of insect physiologyauthors
Robich RM,Rinehart JP,Kitchen LJ,Denlinger DLdoi
10.1016/j.jinsphys.2006.08.008subject
Has Abstractpub_date
2007-03-01 00:00:00pages
235-45issue
3eissn
0022-1910issn
1879-1611pii
S0022-1910(06)00151-Xjournal_volume
53pub_type
杂志文章abstract::Resting potentials in Apis mellifera and Drosophila melanogaster flight muscles decrease with falling temperatures. When resting potentials fall to between -37 and -45 mV they activate a final burst of spontaneous muscle action potentials (MAPs). This final burst of MAPs marks the beginning of chill-coma for each spec...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(99)00148-1
更新日期:2000-05-01 00:00:00
abstract::Dippu-allatostatins (ASTs) have pleiotropic effects in Locusta migratoria. Dippu-ASTs act as releasing factors for adipokinetic hormone I (AKH I) from the corpus cardiacum (CC) and also alter juvenile hormone (JH) biosynthesis and release from the corpus allatum (CA). Dippu-AST-like immunoreactivity is found within la...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2008.03.007
更新日期:2008-06-01 00:00:00
abstract::We investigated if Brindley's and metasternal glands are involved in the sexual behavior of Triatoma infestans. In laboratory assays, we analyzed the effect of selective occlusion of Brindley's and metasternal glands of the female (separately and together) on the behavior of males. Control assays without occlusion of ...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2007.03.014
更新日期:2007-07-01 00:00:00
abstract::We analyzed brush border membrane vesicle proteins from isolated midguts of the mosquito Aedes aegypti, by two proteomic methods: two-dimensional gel electrophoresis (isoelectric focusing and SDS-PAGE) and a shotgun two-dimensional liquid chromatographic (LS/LS) approach based on multidimensional protein identificatio...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2008.12.008
更新日期:2009-03-01 00:00:00
abstract::Pattern discrimination in the honeybee was studied by training alternately with two different pairs of patterns. Individually marked bees made a forced choice from a fixed distance in a standard Y-choice maze for a reward of sugar solution. Bees were trained, first on one pair of patterns for 10min then on a second pa...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(98)00132-2
更新日期:1999-04-01 00:00:00
abstract::Certain male fruit flies from the genera Bactrocera and Zeugodacus (Diptera: Tephritidae) actively forage for floral semiochemicals produced by some endemic Bulbophyllum orchids found in tropical and subtropical forests. These floral semiochemicals are largely classified as either phenylbutanoids (e.g., raspberry keto...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2019.103949
更新日期:2019-01-01 00:00:00
abstract::Humidity has a large impact on the distribution and abundance of terrestrial invertebrates, but the molecular mechanisms governing drought resistance are not fully understood. Some attention has been given to the role of the heat shock response as a component of desiccation tolerance, but recent focus has been on the ...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2008.11.010
更新日期:2009-03-01 00:00:00
abstract::Facultative diapause, a strategy that allows insects to initiate additional generations when conditions are favorable or to enter diapause when they are not, has a profound effect on the ecology and evolution of species. Most previous studies have concentrated on the role of photoperiod and temperature in inducing fac...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2010.08.007
更新日期:2010-12-01 00:00:00
abstract::Temperature can modulate the responses of ectotherms to environmental stressors, such as food shortage. Temperature-mediated plasticity in starvation resistance can arise by changes in the amount of energy stored, the speed of energy expenditure, or the threshold energy reserves required for survival. However, few stu...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2018.08.004
更新日期:2018-10-01 00:00:00
abstract::Insect molting and metamorphosis are controlled by the molt stimulating hormone ecdysone. A recent study suggests that reduced tissue oxygenation correlates with the size-sensing mechanism responsible for triggering molting. When reared in hypoxia, larvae of Manduca sexta and Drosophila melanogaster initiate molting a...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2015.02.009
更新日期:2015-05-01 00:00:00
abstract::Disruption of the appropriate balance between juvenile hormone (JH) and ecdysteroids causes abnormal insect development. The application of a JH analog (JHA) during the early days of the final (fifth) instar induces dauer larvae with low ecdysteroid titers in insects, but the mechanism that underlies the action of JHA...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2015.03.012
更新日期:2015-09-01 00:00:00
abstract::Blood-sucking insects strongly rely on olfactory cues to find their vertebrate hosts. As in other insects with different lifestyles, it has been shown that endogenous and exogenous factors modulate olfactory responses. The triatomine bug Rhodnius prolixus is an important vector of Chagas disease and a classical model ...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2014.09.009
更新日期:2014-12-01 00:00:00
abstract::The synergistic effect between the entomopathogenic fungus Metarhizium robertsii and a sublethal dose of the bacterium Bacillus thuringiensis ssp. morrisoni var. tenebrionis was studied in terms of immune defense reactions and detoxification system activity of the Colorado potato beetle, Leptinotarsa decemlineata, fou...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2016.10.004
更新日期:2017-01-01 00:00:00
abstract::First generation hybrid males from crosses between the Asian corn borer (ACB), Ostrinia furnacalis, and the "univoltine Z-strain" European corn borer (ECB), Ostrinia nubilalis, were examined with respect to behavioral and physiological responses to ACB and ECB pheromones. The hybrid males often flew to the pheromone o...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2009.09.005
更新日期:2010-01-01 00:00:00
abstract::The hymenopteran Macrocentrus cingulum usually deposits one egg into the larval body cavity of lepidopteran Ostrinia furnacalis, and the egg subsequently splits into several dozens of embryos during its development. How the parasitoid eggs and embryos avoid encapsulation by the host's immune response remains unknown. ...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(03)00021-0
更新日期:2003-04-01 00:00:00
abstract::Mating in moths is generally mediated by female-produced sex pheromones. Mating activity, female pheromone production/release and male pheromone responsiveness all show diurnal variations in many species. We found that the response of the male Egyptian cotton leafworm, Spodoptera littoralis, to sex pheromone gland ext...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2004.11.013
更新日期:2005-03-01 00:00:00
abstract::The flightless bug Pyrrhocoris apterus (L.) is polymorphic for both wing length and flight muscle development. The developed flight muscles of macropterous adults of both sexes first enlarge their volume during the first 5 days after adult emergence, but are then histolyzed in all males and females older than 10 and 1...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2005.10.009
更新日期:2006-03-01 00:00:00
abstract::Uvarov's theory of locust phase polymorphism implies a differential phase-specific gene expression. It was the goal of the present investigation to provide data on the molecular level for this concept. We used the technique of 2D gel electrophoresis to generate haemolymph polypeptide maps from mature locust males. Und...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(99)00095-5
更新日期:1999-12-01 00:00:00
abstract::Studies were undertaken to investigate vitellogenesis and its regulation in female adults of the fall armyworm, Spodoptera frugiperda. A single female-specific protein, likely to be the S. frugiperda vitellogenin (Vg), appeared approximately 5 h after adult eclosion in the hemolymph of virgin females. The concentratio...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(99)00207-3
更新日期:2000-06-01 00:00:00
abstract::The study of copulation and the control of egg production in the blood-feeding insect, Rhodnius prolixus, continues to offer a fertile ground for the cultivation of new insights into the physiological processes associated with reproduction. We begin this paper by describing the male organs of copulation showing that t...
journal_title:Journal of insect physiology
pub_type: 杂志文章,评审
doi:10.1016/j.jinsphys.2016.06.001
更新日期:2017-01-01 00:00:00
abstract::Wings and legs of the gregarious desert locust, Schistocerca gregaria have been shown to be release sites of phenylacetonitrile (PAN), the major adult male-produced pheromone. However, there is limited information on the distribution of PAN within the locust. Here we show, using gas chromatography-mass spectrometry (G...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2012.03.012
更新日期:2012-08-01 00:00:00
abstract::Force feeding of RH-5992 (Tebufenozide), a non-steroidal ecdysone agonist to newly moulted sixth instar larvae of the spruce budworm, Choristoneura fumiferana, (Lepidoptera: Tortricidae) initiates a precocious, incomplete moult. Within 6h post treatment (pt) the larva stops feeding and remains quiescent. Around 12hpt,...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(96)00062-5
更新日期:1997-02-19 00:00:00
abstract::The two-spotted spider mite, Tetranychus urticae was exposed to UV-C (250 nm), UV-B (300 nm), and UV-A (350 nm). In non-diapausing females, the median effective doses for 50% mortality plus escape incidence (ED(50)) were 21 (UV-C) and 104 kJm(-2) (UV-B); those for 50% oviposition rate in continuous darkness-treated mi...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2009.04.005
更新日期:2009-07-01 00:00:00
abstract::Electroantennogram (EAG) responses to the aphid sex pheromone components, (1R,4aS,7S,7aR)-nepetalactol and (4aS,7S,7aR)-nepetalactone, and a plant volatile, (E)-2-hexenal, were investigated at three different positions (5/6th, 4/5th and 3/4th inter-segmental regions) along the antennae of four different morphs in two ...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(02)00082-3
更新日期:2002-05-01 00:00:00
abstract::The clock protein PERIOD (PER) displays circadian cycles of accumulation, phosphorylation, nuclear translocation and degradation in Drosophila melanogaster clock cells. One exception to this pattern is in follicular cells enclosing previtellogenic ovarian egg chambers. In these cells, PER remains high and cytoplasmic ...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2009.01.017
更新日期:2009-05-01 00:00:00
abstract::We have investigated the effect of the locust myosuppressin, SchistoFLRFamide, on the activity of amylase and alpha-glucosidase in the midgut of 2-week old male locusts. Total enzyme activity in the lumen contents and tissue extracts of midguts responds to SchistoFLRFamide in a dose-dependent manner that appears to va...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2004.10.003
更新日期:2005-01-01 00:00:00
abstract::Sublethal concentrations of the bisacylhydrazine moulting hormone agonists, RH-5849, and tebufenozide (RH-5992) were fed to sixth (final) instar larvae of Spodoptera litura. Both RH-5849 and tebufenozide adversely affected the mating success of S. litura when the surviving treated males were crossed with normal female...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2004.03.007
更新日期:2004-06-01 00:00:00
abstract::In this study, we investigated activation of the extracellular signal-regulated kinase (ERK) by the prothoracicotropic hormone (PTTH) in prothoracic gland cells of the silkworm, Bombyx mori. The results showed that the PTTH stimulated ERK phosphorylation as this depends on time and dose and ecdysteroidogenic activity....
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2007.03.004
更新日期:2007-06-01 00:00:00
abstract::Oocyte counts, used as a measure of egg load, were compared among three different age groups (15, 30 and 45 days) of two polyphagous species of tephritid fruit flies, Anastrepha ludens and Anastrepha obliqua, which were exposed to varying conditions of diet (sucrose vs sucrose and protein), availability of oviposition...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(01)00072-5
更新日期:2001-09-01 00:00:00
abstract::The corpora cardiaca (CC) of the Italian race (including also the africanised variety) of the honeybee (Apis mellifera ligustica) contain approximately 3 pmol of a hypertrehalosaemic peptide. This peptide is identical in structure to the adipokinetic hormone (AKH) found in Manduca sexta, Mas-AKH. The CC of the dark Eu...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(98)00158-9
更新日期:1999-07-01 00:00:00