Abstract:
:Insect molting and metamorphosis are controlled by the molt stimulating hormone ecdysone. A recent study suggests that reduced tissue oxygenation correlates with the size-sensing mechanism responsible for triggering molting. When reared in hypoxia, larvae of Manduca sexta and Drosophila melanogaster initiate molting at lower weights than do larvae reared in normoxia. Furthermore, in Drosophila, the signaling gas nitric oxide (NO) appears to be required for normal developmental timing. As observed in Drosophila, NO signaling targets the nuclear hormone receptor beta fushi tarazu transcription factor 1 (βFTZ-F1) through activation of Drosophila hormone receptor 3 (DHR3), two key regulators of ecdysone production and metamorphic tissue progression. We set out to directly examine the effects of hypoxia and NO on ecdysone secretion using prothoracic glands from feeding fifth (last) larval stage M. sexta. Our results indicate that in vitro treatment of prothoracic glands with hypoxia (2% oxygen) or the NO donor DETA-NONOate significantly inhibit ecdysone secretion. Protein markers of glandular activity were also in keeping with an initial inhibition, measured a decrease in phosphorylated ERK (extracellular signal regulated kinase) and an increase in non-phosphorylated 4EBP (eukaryotic initiation factor 4E binding protein). Additionally, gene expression levels of Manduca hormone receptor 3 (mhr3), βftz-f1, nitric oxide synthase (nos), and the PTTH receptor torso, were quantified using real-time PCR. NO treatment increased mhr3 expression and decreased nos expression. Hypoxia increased mhr3 transcription after 2 hr, but decreased transcription after 12 hr, with no effect on nos expression. Both NO and hypoxia had small effects on βftz-f1 expression, yet strongly increased torso transcription. Our results demonstrate that, in isolated prothoracic glands, hypoxia and NO signaling directly inhibit ecdysteroid secretion, but at the same time alter aspects of prothoracic gland function that may enhance secretory response.
journal_name
J Insect Physioljournal_title
Journal of insect physiologyauthors
DeLalio LJ,Dion SM,Bootes AM,Smith WAdoi
10.1016/j.jinsphys.2015.02.009subject
Has Abstractpub_date
2015-05-01 00:00:00pages
56-66eissn
0022-1910issn
1879-1611pii
S0022-1910(15)00044-Xjournal_volume
76pub_type
杂志文章abstract::Elucidating the mechanisms by which honey bees process pollen vs. protein supplements are important in the generation of artificial diets needed to sustain managed honeybees. We measured the effects of diet on protein concentration, hypopharyngeal gland development and virus titers in worker honey bees fed either poll...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2010.03.017
更新日期:2010-09-01 00:00:00
abstract::Nestmate recognition in ants is based on perceived differences in a multi-component blend of hydrocarbons that are present on the insect cuticle. Although supplementation experiments have shown that some classes of hydrocarbons, such as methyl branched alkanes and alkenes, have a salient role in nestmate recognition, ...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2011.10.010
更新日期:2012-01-01 00:00:00
abstract::By single sensillum tip recording technique, in addition to the salt and pH cells found in antennal taste sensilla of some ground beetles earlier, the third chemosensory cell of four innervating these large sensilla was electrophysiologically identified as a sugar cell in the ground beetle Pterostichus aethiops. This ...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2006.12.012
更新日期:2007-04-01 00:00:00
abstract::Flight behaviors in various insect species are closely correlated with their mechanical and neuronal properties. Compared to locusts and flies which have been intensively studied, moths have "intermediate" properties in terms of the neurogenic muscle activations, power generation by indirect muscles, and two-winged-in...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2011.08.002
更新日期:2011-11-01 00:00:00
abstract::An animal's phenotype may be shaped by its genes, but also reflects its own environment and often that of its parents. Nongenetic parental effects are often mediated by steroid hormones, and operate between parents and offspring through mechanisms that are well described in vertebrate and model systems. However, less ...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2018.06.003
更新日期:2018-01-01 00:00:00
abstract::Atmospheric oxygen is one of the most important atmospheric component for all terrestrial organisms. Variation in atmospheric oxygen has wide ranging effects on animal physiology, development, and evolution. This variation in oxygen has the potential to affect both respiratory systems (the supply side) and mitochondri...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2017.11.001
更新日期:2018-04-01 00:00:00
abstract::In comparison to other similarly sized mantis species examined in previous studies, Euchomenella macrops has a significantly smaller head, shorter foreleg tibia, but longer prothorax which have been interpreted as specializations for the capture of smaller, slower prey. We tested this conjecture by assessing the rates...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2012.01.018
更新日期:2012-05-01 00:00:00
abstract::The behaviour of those polistine wasps which found their nests by swarming, suggests that these species use trail pheromones for leading the swarm to the location chosen for the new nest. Apart from a recent report regarding the ropalidine Polybioides tabidus, where the pheromone is thought to originate from the Dufou...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(99)00059-1
更新日期:1999-10-01 00:00:00
abstract::In Drosophila multiple circadian oscillators and behavioral rhythms are known to exist, yet most previous studies that attempted to understand circadian entrainment have focused on the activity/rest rhythm and to some extent the adult emergence rhythm. Egg laying behavior of Drosophila females also follows circadian r...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2011.11.012
更新日期:2012-02-01 00:00:00
abstract::When non-diapause and diapause pupae of Delia antiqua were exposed to various thermoperiods where thermophase (T) was 25 degrees C and the cryophase (C) was 15 or 20 degrees C (TC(15) or TC(20)) in constant darkness (DD), the majority of both types of flies emerged before the rise in temperature. Eclosion time was del...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(02)00157-9
更新日期:2002-09-01 00:00:00
abstract::Asobara japonica (Braconidae) is an endophagous parasitoid developing in Drosophila larvae. The present study shows that A. japonica was never encapsulated in Drosophila melanogaster, and that it caused an overall inhibition of the host encapsulation reaction since injected foreign bodies were never encapsulated in pa...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2009.09.001
更新日期:2010-01-01 00:00:00
abstract::RNA interference (RNAi) strategy, which enables gene-specific knock-down of transcripts, has been spread across a wide area of insect studies for investigating gene function without regard to model and non-model insects. This technique is of particular benefit to promote molecular studies on non-model insects. However...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2013.02.009
更新日期:2013-04-01 00:00:00
abstract::The two-spotted spider mite, Tetranychus urticae was exposed to UV-C (250 nm), UV-B (300 nm), and UV-A (350 nm). In non-diapausing females, the median effective doses for 50% mortality plus escape incidence (ED(50)) were 21 (UV-C) and 104 kJm(-2) (UV-B); those for 50% oviposition rate in continuous darkness-treated mi...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2009.04.005
更新日期:2009-07-01 00:00:00
abstract::Phosphorylation of the translational repressor 4E-binding protein (4E-BP) plays a critical role in regulating the overall translation levels in cells. In the present study, we investigated 4E-BP phosphorylation of Bombyx mori eggs by an immunoblot analysis of a conserved phospho-specific antibody to 4E-BP and demonstr...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2011.04.014
更新日期:2011-07-01 00:00:00
abstract::Copula duration and sperm storage patterns can directly or indirectly affect fitness of male and female insects. Although both sexes have an interest in the outcome, research has tended to focus on males. To investigate female influences, we compared copula duration and sperm storage of Queensland fruit fly females th...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2010.07.005
更新日期:2010-12-01 00:00:00
abstract::Plant-derived trypsin inhibitors have been shown to have potent anti-insect effects and are a promising alternative for the biological control of pests. In this work, we tested the anti-insect activity of Adenanthera pavonina trypsin inhibitor (ApTI) against Diatraea saccharalis larvae, a major insect pest in sugarcan...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2013.11.012
更新日期:2014-02-01 00:00:00
abstract::The intracellular elemental concentrations of K, Na, Cl, P, Mg and Ca within Type I cells of the Malpighian tubules of Locusta migratoria have been measured using electron probe X-ray microanalysis. The distribution of Na, K and Cl was not homogeneous within the cells and concentration gradients exist from basal to ap...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(00)00131-1
更新日期:2001-04-01 00:00:00
abstract::Midgut extracts from Aedes aegypti females exhibited hydrolytic activities against synthetic substrates for carboxypeptidase A, carboxyopeptidase B and leucine-aminopeptidase. The three activities showed a broad pH optimum, with maximum activities at pH between 6.5 and 8.5. Enzymatic activities were further characteri...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(01)00165-2
更新日期:2002-02-01 00:00:00
abstract::The regulation of caste differentiation is essential to insect eusociality. Termite soldiers are sterile and cannot eat by themselves because they have specialized mouth morphology. Almost all termite species have a soldier caste, and the soldier ratio per colony is maintained at a low level, probably by elaborate reg...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2011.03.005
更新日期:2011-06-01 00:00:00
abstract::In insects, especially in mosquitoes that are adult blood feeders, midgut remodeling is an important event during metamorphosis. It involves two processes viz., programmed cell death (PCD) of larval cells, and proliferation and differentiation of imaginal cells to form pupal/adult midgut. These processes are regulated...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2006.09.009
更新日期:2007-03-01 00:00:00
abstract::Metabolic respiration by groups of resting Myrmica ruginodis and M. scabrinodis worker ants from five sites representing a range of latitudes, have been compared by measuring rates of CO(2) production-standardised by fat-free weight-at 5 and 25 degrees C. M. ruginodis which lives in cooler habitats than M. scabrinodis...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(98)00162-0
更新日期:1999-06-01 00:00:00
abstract::Pemphigus bursarius (L.) is a host alternating root-feeding aphid with a proportion of the population overwintering as asexual hiemalis in the soil. These hiemalis must be sufficiently cold tolerant to survive at the temperatures they would experience in winter, and also be able to overcome a period of prolonged starv...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(99)00188-2
更新日期:2000-03-01 00:00:00
abstract::The pierid butterfly Colias croceus (Geoffroy in Fourcroy, 1785), established in the Azores archipelago, is polymorphic with six forms, C. croceus f. croceus ♂ and ♀, C. c. f. cremonae ♂ and ♀, C. c. f. helice ♀, and C. c. f. cremonaehelice ♀. We investigated the optical mechanisms underlying the wing colouration of t...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2020.104114
更新日期:2020-01-01 00:00:00
abstract::Tri-trophic impacts on adult predatory carabid beetles, Ctenognathus novaezelandiae, of insect-resistant transgenic tobacco plants expressing a serine protease inhibitor, bovine spleen trypsin inhibitor (BSTI), or a biotin-binding protein, avidin, were investigated. Both proteins could potentially affect this beetle, ...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2007.12.002
更新日期:2008-02-01 00:00:00
abstract::Social environments can have a major impact on ageing profiles in many animals. However, such patterns in variation in ageing and their underlying mechanisms are not well understood, particularly because both social contact and isolation can be stressful. Here, we use Drosophila melanogaster fruitflies to examine sex-...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2017.08.008
更新日期:2017-10-01 00:00:00
abstract::Globally increasing temperatures may strongly affect insect herbivore performance. In contrast to direct effects of temperature on herbivores, indirect effects mediated via thermal effects on host-plant quality are only poorly understood, despite having the potential to substantially impact the herbivores' performance...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2014.03.003
更新日期:2014-05-01 00:00:00
abstract::The ability of organisms to adapt to fluctuating food conditions is essential for their survival and reproduction. Accumulating energy reserves, such as lipids, in anticipation of harsh conditions, will reduce negative effects of a low food supply. For Hymenoptera and Diptera, several parasitoid species lack adult lip...
journal_title:Journal of insect physiology
pub_type: 杂志文章,评审
doi:10.1016/j.jinsphys.2008.07.014
更新日期:2008-09-01 00:00:00
abstract::Larvae of the west-Japan type yellow-spotted longicorn beetle, Psacothea hilaris (Coleoptera: Cerambycidae), show a long-day type photoperiodic response at 25 degrees C; under long-day conditions, larvae pupate after the fourth or fifth instar, while under short-day conditions, they undergo a few nonstationary supernu...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(03)00167-7
更新日期:2003-10-01 00:00:00
abstract::Elucidating genes that affect life span or that can be used as biomarkers for ageing has received attention in diverse studies in recent years. Using model organisms and various approaches several genes have been linked to the longevity phenotype. For Drosophila melanogaster those studies have usually focussed on one ...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2013.01.005
更新日期:2013-03-01 00:00:00
abstract::Chemosensory proteins (CSPs) are a family of small soluble proteins that, in addition to the odorant-binding proteins (OBPs), are involved in chemical communication. To understand the physiological function of the 16 known CSPs in the silkworm Bombyx mori, we investigated the expression patterns in different tissues a...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2013.04.004
更新日期:2013-07-01 00:00:00