Abstract：
:The insect peritrophic membrane (PM) is a non-cellular structure composed of secreted proteins imbedded in a proteoglycan matrix together with chitin. It separates the midgut epithelium from the intestinal contents, and functions in the digestion of food. Furthermore it acts as a protective barrier against abrasive particles and microbial infections. Here we studied for the first time the N-glycome of the PM. We identified the N-glycan structures present in the PM of the Colorado potato beetle (CPB) at the fourth larval stage using MALDI-TOF mass spectrometry. In parallel, we correlated the N-glycan data to the presence of the N-glycosylation related genes (NGRGs) in the transcriptome of epithelial midgut cells. The presumed activities of the identified genes support the N-glycan profile resolved for the proteins in the PM. To our knowledge these data are the first report on the N-glycome of the PM of a pest insect. These results will contribute to the study of the importance of N-glycosylation in the function and structure of the PM. In addition, the data can help to find novel targets and design better biopesticides for pest control.
journal_name
J Insect Physioljournal_title
Journal of insect physiologyauthors
Liu D,De Schutter K,Smargiasso N,De Pauw E,Van Damme EJM,Smagghe Gdoi
10.1016/j.jinsphys.2019.03.009subject
Has Abstractpub_date
2019-01-01 00:00:00pages
27-32eissn
0022-1910issn
1879-1611pii
S0022-1910(18)30455-4journal_volume
115pub_type
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